Cy2
(Synonyms: Cyanine2) 目录号 : GC35765
Cy2 （Cyanine2）是一种花青染料，可标记多肽、蛋白和寡核苷酸中的氨基，激发光和发射光波长分别为 488 nm 和 520 nm。
Cas No.:260430-02-2
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cy2 (Cyanine2) is a cyanine dye used for labeling amino-groups in peptides, proteins, and oligonucleotides, with Λ excitation of 488 nm and Λ emission of 520 nm[1].
[1]. Sandberg A, et al. Use of Time-Resolved Fluorescence To Improve Sensitivity and Dynamic Range of Gel-Based Proteomics. Anal Chem. 2016 Mar 15;88(6):3067-74.
| Cas No. | 260430-02-2 | SDF | |
| 别名 | Cyanine2 | ||
| Canonical SMILES | CC[N+]1=C(/C=C/C=C(N2CCCCCC(O)=O)/OC3=C2C=CC=C3)OC4=CC=CC=C41.[I-] | ||
| 分子式 | C25H27IN2O4 | 分子量 | 546.4 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8302 mL | 9.1508 mL | 18.3016 mL |
| 5 mM | 0.366 mL | 1.8302 mL | 3.6603 mL |
| 10 mM | 0.183 mL | 0.9151 mL | 1.8302 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Eicosapentaenoic acid production from Nannochloropsis oceanica Cy2 using deep sea water in outdoor plastic-bag type photobioreactors
Bioresour Technol 2018 Apr;253:1-7.PMID:29328929DOI:10.1016/j.biortech.2017.12.102.
In this study, Nannochloropsis oceanica Cy2 was grown in deep-sea water (DSW)-based medium in 5-L plastic bag-type photobioreactors (PBRs) for the autotrophic production of Eicosapentaenoic acid (EPA, 20:5n-3). EPA production of N. oceanica Cy2 was stimulated when it was grown in 100% DSW amended with 1.5 g L-1 NaNO3, achieving a EPA content of 3.1% and a biomass concentration of 3.3 g L-1. An outdoor-simulated microalgae cultivation system was also conducted to validate the feasibility of outdoor cultivation of the Cy2 strain in plastic bag-type PBRs. Using an inoculum size of 0.6 g/L, the biomass concentration in the PBR culture was 3.5 g L-1, while the EPA content and productivity reached a maximal level of 4.12% and 7.49 mg L-1 d-1, respectively. When the PBRs were operated on semi-batch mode, the EPA productivity could further increase to 9.9 mg L-1 d-1 with a stable EPA content of 4.1%.
Toxicity of busulfan and cyclophosphamide (BU/Cy2) in patients with hematologic malignancies
Bone Marrow Transplant 1996 Mar;17(3):329-33.PMID:8704682doi
Between July 1991 and January 1994, 52 patients with hematologic malignancies underwent BMT using BU/Cy2 as conditioning regimen. Median patient age was 38 years. Eleven patients underwent autologous BMT, 22 HLA-identical allogeneic BMT, and 19 patients underwent a MUD or an allogeneic mismatched BMT. GVHD prophylaxis was with cyclosporine/methylprednisone in 26 patients; T cell depletion was used in 15 patients. VOD was observed in 7.5% of patients, IP in 12%, seizures in 4%. The overall incidence of grade II-IV acute GVHD was 35%. Delayed platelet engraftment was observed in seven of 11 patients who underwent autologous BMT. Graft failure was seen in seven of 19 (37%) patients who underwent MUD or allogeneic mismatched BMT. Six of the seven patients received T cell depletion as GVHD prophylaxis. BU/Cy2 transplantation from an unrelated or family-mismatched donor with T cell depletion is associated with a high incidence of graft failure.
Busulfan and cyclophosphamide (BU/Cy2) as preparative regimen for patients with lymphoma
Bone Marrow Transplant 1997 Apr;19(8):777-81.PMID:9134168DOI:10.1038/sj.bmt.1700733.
The combination of busulfan and cyclophosphamide has seldom been employed as a conditioning regimen for patients with lymphoma. Twenty patients with relapsed or refractory lymphoma were treated with busulfan (16 mg/kg) and cyclophosphamide (120 mg/kg) (BU/CY) followed by peripheral blood stem cell rescue in 19 patients or autologous bone marrow in one patient. There were 12 females and eight males, with a median age of 48 years (range 30-65). Four patients had Hodgkin's disease, and 16 patients had non-Hodgkin's lymphoma. Disease status at the time of BU/CY was: first relapse in 10 patients (four patients with chemosensitive disease and six patients with chemoresistant disease), primary refractory disease in six patients, and more advanced disease in four patients. Excessive treatment-related toxicity was not noted. There were no cases of interstitial pneumonitis, but three cases of veno-occlusive disease occurred. At 2 years, the estimated overall survival and event-free survival are 50% and 33%. We concluded that BU/CY seems to have sufficient antilymphoma activity, is devoid of excessive toxicity and warrants further investigation in this patient population.
Engineering strategies for enhancing the production of eicosapentaenoic acid (EPA) from an isolated microalga Nannochloropsis oceanica Cy2
Bioresour Technol 2013 Nov;147:160-167.PMID:23994697DOI:10.1016/j.biortech.2013.08.051.
Microalgae have emerged as promising resources for highly unsaturated fatty acids. In this study, an indigenous microalga identified as Nannochloropsis oceanica Cy2 was grown photoautotrophically to produce eicosapentaenoic acid (EPA; 20:5, n-3). Specific engineering strategies were employed to stimulate EPA accumulation in the microalgal cells. The results show that BG-11 was the most effective medium to grow N. oceanica Cy2, giving an EPA content and biomass concentration of 2.38% (per dry cell weight) and 1.53 g/l. The EPA content nearly doubled when using the optimal nitrogen source (NaNO3) at a concentration of 1.50 g/l. The illumination system also markedly affected the EPA content for the photoautotrophic microalga. When the microalgal culture was illuminated with a red LED, an impressively high EPA content of 5.5% was obtained. Finally, using semi-batch cultures operations with LED-blue illumination, the EPA content of N. oceanica Cy2 was stably maintained at 5.0%.
Enhancing the production of eicosapentaenoic acid (EPA) from Nannochloropsis oceanica Cy2 using innovative photobioreactors with optimal light source arrangements
Bioresour Technol 2015 Sep;191:407-13.PMID:25777066DOI:10.1016/j.biortech.2015.03.001.
Binary combinations of LEDs with four different colors were used as light sources to identify the effects of multiple wavelengths on the production of eicosapentaenoic acid (EPA) by an isolated microalga Nannochloropsis oceanica Cy2. Combining LED-Blue and LED-Red could give the highest EPA productivity of 13.24 mg L(-1) d(-1), which was further enhanced to 14.4 mg L(-1) d(-1) when using semi-batch operations at a 40% medium replacement ratio. A novel photobioreactor with additional immersed light sources improved light penetration efficiency and led to an 38% (0.170-0.235 g L(-1) d(-1)) increase in the microalgae biomass productivity and a 9% decrease in electricity consumption yield of EPA (10.15-9.33 kW-h (g EPA)(-1)) when compared with the control (i.e., without immersed light sources). Operating the immersed LEDs at a flashing-frequency of 9 Hz further lowered the energy consumption yield to 8.87 kW-h (g EPA)(-1).