p-Nitrobenzyl mesylate
(Synonyms: 4-Nitrobenzyl mesylate) 目录号 : GC44663
A reagent that is used to alkylate thiophosphates
Cas No.:39628-94-9
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
p-Nitrobenzyl mesylate (PNBM) is a reagent that is used to alkylate thiophosphates, forming thiophosphate ester epitopes that can be recognized by specific antibodies.[1] Substrates phosphorylated with adenosine 5'-(γ-thio)-triphosphate or N6-benzyl-ATPγS can be alkylated by PNBM.[2],[3] The tagged substrates can be isolated by immunoprecipitation or immunoaffinity purification, or they can be detected by immunoblotting.
p-Nitrobenzyl mesylate (PNBM) 是一种试剂，用于烷基化硫代磷酸盐，形成可被特异性抗体识别的硫代磷酸酯表位。 [1]用腺苷 5'-(γ-硫代)-三磷酸或 N6-苄基-ATPγS 磷酸化的底物可以被 PNBM 烷基化。[2],[3] 标记的底物可以通过免疫沉淀或免疫亲和纯化分离，或者可以检测它们通过免疫印迹。
Reference:
[1]. Allen, J.J., Li, M., Brinkworth, C.S., et al. A semisynthetic epitope for kinase substrates. Nat. Methods 4(6), 511-516 (2007).
[2]. Kumar, V., Weng, Y.-C., Geldenhuys, W.J., et al. Generation and characterization of ATP analog-specific protein kinase Cδ. J. Biol. Chem. 290(4), 1936-1951 (2015).
[3]. Leissing, F., Nomoto, M., Bocola, M., et al. Substrate thiophosphorylation by Arabidopsis mitogen-activated protein kinases. BMC Plant Biol. 16(48), (2016).
| Cas No. | 39628-94-9 | SDF | |
| 别名 | 4-Nitrobenzyl mesylate | ||
| 化学名 | 4-nitro-benzenemethanol, 1-methanesulfonate | ||
| Canonical SMILES | CS(OCC1=CC=C([N+]([O-])=O)C=C1)(=O)=O | ||
| 分子式 | C8H9NO5S | 分子量 | 231.2 |
| 溶解度 | 100mM in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 4.3253 mL | 21.6263 mL | 43.2526 mL |
| 5 mM | 0.8651 mL | 4.3253 mL | 8.6505 mL |
| 10 mM | 0.4325 mL | 2.1626 mL | 4.3253 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Atg11 is required for initiation of glucose starvation-induced autophagy
Autophagy 2020 Dec;16(12):2206-2218.PMID:31971848DOI:10.1080/15548627.2020.1719724.
How energy deprivation induces macroautophagy/autophagy is not fully understood. Here, we show that Atg11, a receptor protein for cargo recognition in selective autophagy, is required for the initiation of glucose starvation-induced autophagy. Upon glucose starvation, Atg11 facilitates the interaction between Snf1 and Atg1, thus is required for Snf1-dependent Atg1 activation. Phagophore assembly site (PAS) formation requires Atg11 via its control of the association of Atg17 with Atg29-Atg31. The binding of Atg11 with Atg9 is crucial for recruiting Atg9 vesicles to the PAS and, thus, glucose starvation-induced autophagy. We propose Atg11 as a key initiation factor controlling multiple key steps in energy deprivation-induced autophagy. Abbreviations: AMPK: AMP-activated protein kinase; Ams1: α-mannosidase; Ape1: aminopeptidase I; Cvt: cytoplasm-to-vacuole targeting; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; GFP: green fluorescent protein; MBP: myelin basic protein; MMS: methanesulfonate; PAS: phagophore assembly site; PNBM: p-Nitrobenzyl mesylate; SD-G: glucose starvation medium; SD-N: nitrogen starvation medium; ULK1, unc-51 like autophagy activating kinase 1; WT: wild type.