Aloin(mixture of A&B)
(Synonyms: 芦荟苷) 目录号 : GC35300
An anthroquinone with diverse biological activities
Cas No.:8015-61-0
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Aloin is a mixture of aloin A and aloin B , anthraquinones found in Aloe vera, and has diverse biological activities.1,2,3 It reduces IL-6 and TNF-α expression as well as nitric oxide (NO) production and COX-2 and inducible NO synthase (iNOS) expression in LPS-stimulated RAW 264.7 cells when used at concentrations ranging from 100 to 400 mM.1 Aloin inhibits LPS-induced p65 acetylation, nuclear translocation of NF-kB, and apoptosis in RAW 264.7 cells. It reduces motility of T. congolense in vitro and reduces the number of parasites in T. congolense-infected mice when administered at a dose of 400 mg/kg.2 Aloin (50 and 100 mg/kg) also reduces activity of glutathione peroxidase (GPX), glutathione-S-transferase (GST), and glutathione reductase (GR), the level of colonic malondialdehyde (MDA), and the number of aberrant crypt foci and mucin-depleted foci in a rat model of 1,2-dimethylhydrazine-induced colon cancer.3
1.Luo, X., Zhang, H., Wei, X., et al.Aloin suppresses lipopolysaccharide-induced inflammatory response and apoptosis by inhibiting the activation of NF-κBMolecules23(3)E517(2018) 2.Tewabe, Y., Bisrat, D., Terefe, G., et al.Antitrypanosomal activity of aloin and its derivatives against Trypanosoma congolense field isolateBMC Vet. Res.1061(2014) 3.Hamiza, O.O., Rehman, M.U., Khan, R., et al.Chemopreventive effects of aloin against 1,2-dimethylhydrazine-induced preneoplastic lesions in the colon of Wistar ratsHum. Exp. Toxicol.33(2)148-163(2014)
| Cas No. | 8015-61-0 | SDF | |
| 别名 | 芦荟苷 | ||
| Canonical SMILES | O=C1C2=C(C=CC=C2O)C([C@H]3[C@@H]([C@H]([C@@H]([C@@H](CO)O3)O)O)O)C4=CC(CO)=CC(O)=C14 | ||
| 分子式 | C21H22O9 | 分子量 | 418.39 |
| 溶解度 | Soluble in DMSO | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.3901 mL | 11.9506 mL | 23.9011 mL |
| 5 mM | 0.478 mL | 2.3901 mL | 4.7802 mL |
| 10 mM | 0.239 mL | 1.1951 mL | 2.3901 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
The absence of genotoxicity of a mixture of Aloin A and B and a commercial aloe gel beverage
Toxicol Mech Methods 2022 Jun;32(5):385-394.PMID:34979868DOI:10.1080/15376516.2021.2023828.
Aloe products are increasingly valued as ingredients in food supplements and as flavoring agents. The global Aloe vera market is varied, large, growing, and increasingly important in food, cosmetics, and medicines. Aloin, an anthraquinone glycoside, is one of the major components by weight of the anthraquinone derivatives of Aloe vera gel. Principal metabolites, aloe emodin and emodin, are a source of debate concerning toxic vs salutary effects, hence the accurate toxicological characterization of these compounds has become increasingly important. The purpose of this study was to determine the genotoxic profile of a stabilized Aloe vera juice product derived from the inner filet and marketed as a beverage currently sold in the European Union containing 8 to 10 ppm Aloin and a mixture of purified Aloin A and B. The present data confirm that a commercial stabilized Aloe vera gel intended for consumption as a juice beverage is not genotoxic. Furthermore, both Aloin A and B were negative in the same assays and therefore are also not genotoxic. These results are consistent with the work of other groups and contrast with data obtained using products containing the Aloe vera latex hydroxyanthracene derivatives (HADs).
Evaluation of anti-infective potencies of formulated Aloin A ointment and Aloin A isolated from Aloe barbadensis Miller
BMC Chem 2020 Feb 7;14(1):8.PMID:32047877DOI:10.1186/s13065-020-0659-7.
Introduction: Isolated bioactive components of plants or their raw extract are utilized as complementary or alternate remedy in copious illnesses. The current research was aimed at assessing the activity of Aloin A isolated from Aloe barbadensis Miller and its formulated ointment against six (6) selected clinical isolates. Methods: The column chromatography was utilized in isolating Aloin A from chloroform/methanol solvent polarity. The characterization of the isolated compound was performed by spectroscopy techniques corresponding to UV, IR, 1H- and 13C-NMR spectroscopy. It was formulated as ointment using polyethylene glycol (PEG) and both the ointment and the isolated compound were probed for in vitro antimicrobial activity. Results: Aloin A has been isolated from chloroform/methanol solvent mixture. The structure has been explicated as (10S)-10-β-d-glucopyranosyl-1,8-dihydroxy-3-(hydroxymethyl)-9(10H)-anthracenone(1S)-1,5-anhydro-1-[(9S)-4,5-dihydroxy-2-(hydroxymethyl)-10-oxo-9,10-dihydro-9-anthracenyl]-d-glucitol. The minimum inhibitory concentration (MIC) of the isolated Aloin A on the pathogens ranged from 2.5 to 5.0 mg/ml and 0.32 to 5.0 mg/ml for both Aloin A and the formulated ointment respectively. It was further revealed that the activity of Aloin A showed dose dependence against all the test microorganisms. There was no significant difference in the activity of the drug against K. pneumoniae, S. aureus, E. coli, C. albicans and T. flavus (P > 0.05) when the concentration was raised from 2.5 to 5 mg/ml, however, there was significant difference (P ˂ 0.05) in activity against P. aeruginosa. The formulated ointment exhibited dose dependent activity against all test microorganisms. At low concentrations, the ointment showed no significant difference in diameter zone of inhibition against all test microorganisms (P > 0.05) except P. aeruginosa which exhibited a highly significant difference (P < 0.05). Conclusion: Both the isolated Aloin A and its formulated ointment demonstrated substantial inhibition of growth of the pathogenic strains. These findings sturdily suggest that Aloin A is a nascent drug that could be explored as skin and wound transmittable agent.
Plasma, tissue and urinary levels of Aloin in rats after the administration of pure Aloin
Nutr Res Pract 2008 Spring;2(1):17-21.PMID:20126360DOI:10.4162/nrp.2008.2.1.17.
Aloin is a physiologically active anthraquinone present in aloe. There are two isomers of Aloin, Aloin A and Aloin B, occurring as a mixture of diastereomers. The objective of this study was to determine the bioavailability and tissue distribution of Aloin. Rats were gavaged with 11.8g/kg Aloin, and the levels of Aloin and its conjugates were measured in plasma, tissues, and urine. Plasma Aloin level showed a peak at 1hr after the administration and the concentration was 59.07+/-10.5 ng/ml. The 24 h cumulated urinary Aloin was 0.03% of the initial dose. These results suggest that Aloin is absorbed and reaches a peak plasma level within 1-1.5 h after the administration and a significant portion is possibly metabolized or is excreted in feces. These results can apply to the determination of the adequate intake level of aloe and aloe products to achieve the desired biological effect, and to interprete in vitro study results.
Synergic study of α-glucosidase inhibitory action of Aloin and its antioxidant activity with and without camel β-casein and its peptides
Protein Pept Lett 2013 May;20(5):607-12.PMID:23116059DOI:10.2174/0929866511320050015.
Regular consumption of natural antioxidants reduces the risk of developing diseases. Aloin is one of the main active phenolic components of Aloe vera. The main disadvantage of Aloin is its concentration limit of use that causes cell damage. One of the aims of this study was to investigate the antioxidant activity of Aloin in the presence and absence of camel β-casein ( β-CN) and its peptide fractions. The mixture of Aloin, β-CN and peptides showed a very high antioxidant activity in a synergistic manner as compared to each component alone. The alpha ( α)-glucosidase inhibitory activity of Aloin was also investigated in the presence and absence of β-CN and its peptides. Aloin alone is a potent inhibitor of α-glucosidase. The α-glucosidase inhibitory activity of Aloin is reduced in the presence of β-CN or its peptides. The combination of Aloin and β-CN or its peptides makes a high antioxidant functional ingredient.
Microdontin A and B: Two New Aloin Derivatives from Aloe microdonta
Planta Med 1992 Feb;58(1):88-93.PMID:17226442DOI:10.1055/s-2006-961397.
The leaf juice of ALOE MICRODONTA Chiov. is used in Somali traditional medicine as a remedy for jaundice and for topical treatment of skin diseases. Mucilage was precipitated from the fresh juice by addition of ethanol and the supernatant chromatographed on Amberlite XAD-2 to yield a fraction containing anthraquinone derivatives. Flash chromatography on silica gel followed by preparative TLC yielded Aloin A and a new compound. Spectral data (mass, (1)H- and (13)C-NMR) identified this compound as a mixture of Aloin A and B where the glucose of both compounds is esterified with 4-hydroxycinnamic acid at the 6'-position. The two compounds were denoted as microdontin A and B, respectively.