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Fibronectin Human

目录号 : GP23499

纤连蛋白(Fibronectin, FN),也称为纤维连接蛋白,是一种广谱的、天然的细胞粘附因子。

Fibronectin Human Chemical Structure

规格 价格 库存 购买数量
200μg
¥1,050.00
5-10工作日
1mg
¥3,360.00
5-10工作日
10mg
¥25,550.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

产品文档

Quality Control & SDS

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实验参考方法

一、产品溶解和使用

将产品从冰箱取出置于室温平衡至少20min。按照1mg/ml浓度加入适量无菌蒸馏水重悬粉末,静置30min让粉末充分进入溶液。不可振动或涡旋。如果所有溶液不能立即用完,请按单次用量分装并置于-20°C冻存。建议2周内用完溶解产品。不要保存在无霜冰箱,避免多次反复冻融。

二、产品应用

用于细胞培养包被基质的建议浓度为1-5µg/cm2,或用作培养基添加剂的建议浓度为5µg/ml.

三、包被步骤

以下步骤作为参考,依据具体培养体系来确定最佳的包被浓度。

1)用无血清,不含Ca2+,Mg2+的培养基或者缓冲液稀释纤连蛋白储存液到所需的工作浓度,以最小体积完全覆盖培养表面,推荐包被浓度为1-5µg/cm2。比如,若最终包被浓度为5µg/cm2,稀释储存液到50pg/ml,按照1ml/35mm培养皿,3ml/60mm培养皿等加量。

【注意】:由于纤连蛋白溶液中含CAPS,含有Ca2+Mg2+的培养基添加到纤连蛋白中可能会引起不溶的金属氢氧化物产生。如果缓冲体系能将稀释液的pH调至8.0或更低,不会发生此情况。

2)加适量稀释好的纤连蛋白到培养器皿表面。

3)室温孵育1h.

4)吸掉多余纤连蛋白溶液。

5)用去离子水清洗,注意操作过程中不要刮破底层。

6)此时的培养容器即可使用。或许以潮湿状态保存在2-8°C或无菌条件下风干。

注意事项

1)如果操作不当,本品中的某些成分可能会导致健康风险。因此,操作本品时注意做好防护,包括穿好衣服和带上眼罩。

2)为了您的安全和健康,请穿实验服并戴一次性手套操作。

产品描述

纤连蛋白(Fibronectin, FN),也称为纤维连接蛋白,是一种广谱的、天然的细胞粘附因子。纤连蛋白是一种440-500kDa的二聚体糖蛋自,由两个相似的220-250kDa的亚基通过二硫键连接而成。主要以两种形式存在,可溶形式和不可溶形式,前者以二聚体形式存在血浆中,后者以多聚体形式存在于细胞外基质(ECM)和细胞表面。纤连蛋白首要功能与细胞粘附到细胞外基质有关,通过纤连蛋白上的序列Arg-Gly-Asp-Ser(RGDS)来识别细胞相关的跨膜整合素受体从而调控粘附。纤连蛋白的其他结构域也参与这一粘附过程,可能涉及与胶原蛋自、肝素和其他细胞表面粘多糖的相互作用。粘附后纤连蛋白的构象和方向也很重要,对细胞分布和内皮细胞粘附强度上发挥作用。经纤连蛋白包被的培养器皿用来培养BHK、CHO和其他细胞系呈现出更明显的粘附效应。可以说,纤连蛋白参与体内众多生理过程,比如细胞间的粘附和迁移、细胞骨架组装、酪氨酸磷酸化以及肿瘤转移。

本品是经亲和色谱纯化所得的人血浆来源纤连蛋白,以冻干粉形式提供,适用于细胞粘附和细胞铺展。

Product Data

Purity Greater than 95.0% as determined by SDS-PAGE. Source Human Plasma.
Phycical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Shipping Condition Shipped at Room temp.
Solubility Purified fibronectin has a tendency to form insoluble aggregates upon reconstitution. We suggest reconstituting the 1mg Fibronectin with a chaotropic agent such as urea at room temperature at a concentration of 0.2mg/ml using sterile water. Let stand 1-2 hours. The recommended concentration is 4M-5M urea. When using the protein as an attachment factor, wash the urea off after attaching the fibronectin to the growth surface (plate or dish).
Stability Store the lyophilized fibronectin at 4°C . Upon reconstitution fibronectin should be stored at 4°C for 2 weeks and for future use below -18°C .Please prevent freeze-thaw cycles.
Formulation The Fibronectin was lyophilized from a concentrated 1mg/ml solution containing 0.05M Tris Cl pH-7.5 and 0.1M NaCl.

Introduction

Plasma fibronectin level is elevated in severe coronary artery disease. Increased plasma fibronectin levels are related with venous thromboembolism (VTE) particularly in males, and extend the probable association between biomarkers and risk factors for arterial atherothrombosis and VTE. Fibronectin plays a role in several cellular processes, including tissue repair, embryogenesis, blood clotting, and cell migration/adhesion. Fibronectin consists in two main forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the extracellular matrix and 2) as a soluble disulphide linked dimer found in the plasma. The plasma form is produced by hepatocytes, and the ECM form is synthesized by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells. Fibronectin also takes part as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. Fibronectin organizes cellular interaction with the ECM by binding to different components of the extracellular matrix and to membrane-bound Fibronectin receptors on cell surfaces.

Stability

Store the lyophilized fibronectin at 4°C . Upon reconstitution fibronectin should be stored at 4°C for 2 weeks and for future use below -18°C .Please prevent freeze-thaw cycles.