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EIF3F Human

目录号 : GP23381

Eukaryotic Translation Initiation Factor 3F Human Recombinant

EIF3F Human Chemical Structure

规格 价格 库存 购买数量
5μg
¥840.00
5-10工作日
20μg
¥2,030.00
5-10工作日
1mg
¥37,800.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

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产品描述

EIF3F Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 380 amino acids (1-357 a.a) and having a molecular mass of 40kDa. EIF3F is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Data

Purity Greater than 90.0% as determined by SDS-PAGE. Source Escherichia Coli.
Phycical Appearance Sterile Filtered colorless solution. Shipping Condition Shipped with Ice Packs.
Synonyms Eukaryotic Translation Initiation Factor 3; Subunit F; EIF3S5 ; Eukaryotic Translation Initiation Factor 3; Subunit 5 (Epsilon; 47kD); EC 3.4.19.12; Deubiquitinating Enzyme EIF3f; eIF3f; eIF-3-epsilon; EIF3 P47; Eukaryotic Translation Initiation Factor 3 Subunit 5; eIF3-p47; Eukaryotic Translation Initiation Factor 3; Subunit 5 Epsilon; 47kDa.
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MGSMATPAVP VSAPPATPTP VPAAAPASVP APTPAPAAAP VPAAAPASSS DPAAAAAATA APGQTPASAQ APAQTPAPAL PGPALPGPFP GGRVVRLHPV ILASIVDSYE RRNEGAARVI GTLLGTVDKH SVEVTNCFSV PHNESEDEVA VDMEFAKNMY ELHKKVSPNE LILGWYATGH DITEHSVLIH EYYSREAPNP IHLTVDTSLQ NGRMSIKAYV STLMGVPGRT MGVMFTPLTV KYAYYDTERI GVDLIMKTCF SPNRVIGLSS DLQQVGGASA RIQDALSTVL QYAEDVLSGK VSADNTVGRF LMSLVNQVPK IVPDDFETML NSNINDLLMV TYLANLTQSQ IALNEKLVNL.
Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Formulation EIF3F protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol, and 0.4M urea.

Introduction

Eukaryotic Translation Initiation Factor 3F, alsoknown as EIF3F belongs to the eIF-3 subunit F family. EIF3F is a component ofthe eukaryotic translation initiation factor 3 (eIF-3) complex, which is essential for several steps in the initiation of proteinsynthesis. The eIF-3 complex associates with the 40S ribosome andfacilitates the recruitment of eIF-1, eIF-1A, eIF 2: GTP: methionyl-tRNAi andeIF-5 to form the 43S preinitiation complex (43S PIC). Inaddition, the eIF-3 complex stimulates mRNA recruitment to the 43S PIC andscanning of the mRNA for AUG recognition. TheeIF-3 complex is also necessary for disassembly and recycling of posttermination ribosomal complexes and subsequently preventspremature union of the 40S and 60S ribosomal subunits prior to initiation. Among the diseases associated with EIF3F are intrahepaticcholangiocarcinoma, and cholangiocarcinoma.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.