EF24
目录号 : GC64496
EF24是一种新型合成姜黄素类似物。
Cas No.:342808-40-6
Sample solution is provided at 25 µL, 10mM.
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EF24 is a novel synthetic curcumin analog [1]. EF24 induces apoptosis of cancer cells through a redox-dependent mechanism [2]. EF24 has anti-tumor and anti-aging effects [3-4].
In A549 cells, EF24 (0μM, 0.5μM, 1μM, 2μM, 4μM, 8μM, 16μM; 24h, 48h) inhibits the growth of NSCLC cells in vitro [5]. In K562 cells, EF24 (2μM; 24h) exhibits potent cell killing activity in leukemia cell lines [6]. In SKOV-3, EF24 (3μM; 48h) treatment attenuates ovarian cancer cell proliferation and metastasis [7].
In subcutaneous HCC tumor mice model, EF24 (20mg/kg; ip; 21d) inhibits tumor growth and induces apoptosis [8]. In C57/BL6 mice, EF24 (200μg/kg; ip; 14d) significantly inhibited the significant increase in JunB, JunC, and JunD levels in distant lung tissues after infrared irradiation [9]. In DU145 subcutaneous tumor mice model, EF24 (200μg/kg; ip; 4 weeks) treatment significantly slowed tumor growth [10].
References:
[1]. He Y, Li W, Hu G, et al. Bioactivities of EF24, a novel curcumin analog: a review. Frontiers in Oncology. 2018 Dec 11; 8: 614.
[2]. Adams BK, Cai J, Armstrong J, et al. EF24, a novel synthetic curcumin analog, induces apoptosis in cancer cells via a redox-dependent mechanism. Anti-cancer drugs. 2005 Mar 1;16(3):263-275.
[3]. Sazdova I, Keremidarska-Markova M, Dimitrova D, et al. Anticarcinogenic potency of EF24: An overview of its pharmacokinetics, efficacy, mechanism of action, and nanoformulation for drug delivery. Cancers. 2023 Nov 20; 15(22): 5478.
[4]. Li W, He Y, Zhang R, et al. The curcumin analog EF24 is a novel senolytic agent. Aging (Albany NY). 2019 Jan 28;11(2):771.
[5]. Chang M, Shang M, Yuan F, et al. EF24 exerts cytotoxicity against NSCLC via inducing ROS accumulation. Cancer Cell International. 2021 Dec; 21: 1-3.
[6]. Singh AP, Wax N, Duncan J, et al. Genomic Discovery of EF-24 Targets Unveils Antitumorigenic Mechanisms in Leukemia Cells. bioRxiv. 2024 Oct 16: 2024-2010.
[7]. Zhang D, Wang Y, Dong L, et al. Therapeutic role of EF 24 targeting glucose transporter 1‐mediated metabolism and metastasis in ovarian cancer cells. Cancer science. 2013 Dec; 104(12): 1690-1696.
[8]. Liu H, Liang Y, Wang L, et al. In vivo and in vitro suppression of hepatocellular carcinoma by EF24, a curcumin analog. PloS one. 2012 Oct 31; 7(10): e48075.
[9]. Veeraraghavan J, Natarajan M, Awasthi V, et al. EF24, a novel curcumin analogue inhibits oncogenic activation induced by radiation abscopal effect in distant mice lungs. Cancer Research. 2011 Apr 15;71(8_Supplement):4203.
[10]. Yang CH, Yue J, Sims M, et al. The curcumin analog EF24 targets NF-κB and miRNA-21, and has potent anticancer activity in vitro and in vivo. PloS one. 2013 Aug 7; 8(8): e71130.
EF24是一种新型合成姜黄素类似物 [1]。EF24通过氧化还原依赖机制诱导癌细胞凋亡 [2]。EF24具有抗肿瘤和抗衰老作用 [3-4]。
在A549细胞中，EF24（0μM、0.5μM、1μM、2μM、4μM、8μM、16μM；24h、48h）可抑制体外非小细胞肺癌（NSCLC）细胞的生长 [5]。在K562细胞中，EF24（2μM；24h）对白血病细胞系表现出强大的细胞杀伤活性 [6]。在SKOV-3细胞中，EF24（3μM；48h）治疗可减弱卵巢癌细胞的增殖和转移 [7]。
在皮下HCC肿瘤小鼠模型中，EF24（20mg/kg；ip；21d）可抑制肿瘤生长并诱导细胞凋亡 [8]。在C57/BL6小鼠中，EF24（200μg/kg；ip；14d）显著抑制了红外照射后远处肺组织中JunB、JunC和JunD水平的显著升高 [9]。在DU145皮下肿瘤小鼠模型中，EF24（200μg/kg；ip；4周）治疗显著减缓了肿瘤生长 [10]。
| Cell experiment [1]: | |
Cell lines | A549 cells |
Preparation Method | For cell growth/viability (MTT) assays, A549 cells were seeded in standard 96-well plates with 4000 cells and allowed to grow for 24h to ensure attachment. The growth medium was then replaced with a medium that contained EF24 at the final concentration of 0μM, 0.5μM, 1μM, 2μM, 4μM, 8μM and 16μM. |
Reaction Conditions | 0μM, 0.5μM, 1μM, 2μM, 4μM, 8μM, 16μM; 24h, 48h |
Applications | EF24 inhibits the growth of NSCLC cells in vitro. |
| Animal experiment [2]: | |
Animal models | Subcutaneous HCC tumor mice model |
Preparation Method | Approximately 2×106 Hepa1-6 cells in 200µL PBS were injected subcutaneously into each animal’s back (n = 20). EF24 was dissolved in dimethyl sulfoxide and diluted in sodium chloride. Ten days post-injection, EF24-treated mice (n = 10) were injected intraperitoneally (i.p.) with 200µL EF24 at a dose of 20mg/kg/d for 21d. Control mice (n = 10) were injected i.p. with 200µL PBS daily. On day 22, tumors were removed and weighed. |
Dosage form | 20mg/kg; ip; 21d |
Applications | EF24 inhibits tumor growth and induces apoptosis in a subcutaneous HCC tumor model. |
References: | |
| Cas No. | 342808-40-6 | SDF | Download SDF |
| 分子式 | C19H15F2NO | 分子量 | 311.33 |
| 溶解度 | 储存条件 | Store at -20°C | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.212 mL | 16.0601 mL | 32.1203 mL |
| 5 mM | 0.6424 mL | 3.212 mL | 6.4241 mL |
| 10 mM | 0.3212 mL | 1.606 mL | 3.212 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet