DU717
目录号 : GC32647
DU-717是一种抗高血压剂。
Cas No.:59943-31-6
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Animal experiment: | Rats[2] DU-717 (100mg/kg per day, p.o.)-treated SHRSP, SHRSP fed on a HFC diet (including 20% suet, 5% cholesterol and 2% cholic acid) with 1% NaCl in the drinking water, ALR and NAR, 15 rats in each group, are used for EEG recording. Rats, anesthetized with intraperitoneal injection of pentobarbital (30mg/kg), are placed in a stereotaxic frame for exposing the skull, and 6 small pores of 1mm in diameter are made by a dental drill at frontal, parietal, and occipital areas on both sides. Then, stainless steel electrodes (220μ in diameter), insulated except a small loop which is formed at the tip in order to avoid any injury to the dura and the cortex, are inserted through the pores of the skull and settled gently on the dura. Electrodes on both sides are located 2mm apart from mid-sagittal line, and frontal (F), parietal (P), occipital (O) electrodes are located 5mm, 2.5 mm, and 0mm anterior to the interauricular line, respectively. The indifferent electrode (I) is placed on the mid-sagittal line, 4mm anterior to the bregma. All electrodes are connected with insulated copper wires to a miniature receptacle, and the whole assembly is embedded in dental cement. Two weeks after the operation, EEG is repeatedly recorded for several months. When EEG is recorded, rats are in a clear plastic cage (30×40×40cm) and could move freely. EEG is recorded by a ME-920, connected to an autoanalyzer MAF-5, and a photic stimulator is MS-2PS. Monopolar (I-F, I-P, I-O), and bipolar (F-P, P-O, O-F) leading are recorded. EEG is recorded during wakefulness and drowsiness. The recording calibration is 3cm/sec and 100μV/4mm. |
References: [1]. Yamaguchi T, et al. Determination of 7-chloro-3-(4-methyl-1-piperazinyl)-4h-1,2,4-benzothiadiazine-1,1-dioxide (DU-717) in plasma using electron-capture gas chromatography. J Chromatogr. 1978 Oct 11;160(1):181-90. | |
DU-717 is an antihypertensive agent.
A gas chromatographic method has been developed which enables accurate determination of a new antihypertensive agent, DU-717, in plasma. Accurate determinations are possible over a concentration range from 10 to 150 ng/mL of DU-717 in plasma at a relative standard deviation of 6.2%. The minimum detectable concentration is 1 ng/mL. Plasma levels of DU-717 in spontaneously hypertensive and normotensive rats following single oral administrations (10 mg/kg) have also been determined[1].
[1]. Yamaguchi T, et al. Determination of 7-chloro-3-(4-methyl-1-piperazinyl)-4h-1,2,4-benzothiadiazine-1,1-dioxide (DU-717) in plasma using electron-capture gas chromatography. J Chromatogr. 1978 Oct 11;160(1):181-90. [2]. R Horie. rats (ALR) and normotensive atherogenic rats (NAR).
| Cas No. | 59943-31-6 | SDF | |
| Canonical SMILES | CN1CCN(C2=NC3=CC=C(Cl)C=C3S(N2)(=O)=O)CC1 | ||
| 分子式 | C12H15ClN4O2S | 分子量 | 314.79 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.1767 mL | 15.8836 mL | 31.7672 mL |
| 5 mM | 0.6353 mL | 3.1767 mL | 6.3534 mL |
| 10 mM | 0.3177 mL | 1.5884 mL | 3.1767 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Determination of 7-chloro-3-(4-methyl-1-piperazinyl)-4h-1,2,4-benzothiadiazine-1,1-dioxide (DU-717) in plasma using electron-capture gas chromatography
J Chromatogr 1978 Oct 11;160(1):181-90.PMID:730789DOI:10.1016/s0021-9673(00)91794-1.
A gas chromatographic method has been developed which enables accurate determination of a new antihypertensive agent, DU-717, in plasma. DU-717 is first extracted with ethyl acetate and, after a clean-up procedure, derivatized with peracetic acid followed by diazomethane to form 2-methyl DU-717 N-oxide (direct methylation leads to mixtures). The N-oxide is then pyrolyzed to 2-methyl DU-717 on a gas chromatograph equipped with electron-capture detection. Accurate determinations are possible over a concentration range from 10 to 150 ng/ml of DU-717 in plasma at a relative standard deviation of 6.2%. The minimum detectable concentration is 1 ng/ml. Plasma levels of DU-717 in spontaneously hypertensive and normotensive rats following single oral administrations (10 mg/kg) have also been determined.