D-α-Tocopherol Succinate
(Synonyms: Vitamin E succinate) 目录号 : GC66824
D-α-Tocopherol Succinate (Vitamin E succinate) 是一种抗氧化生育酚，是维生素 E 的一种盐形式。D-α-Tocopherol Succinat 抑制 Cisplatin 引起的毒性。D-α-Tocopherol Succinate 可用于癌症的研究。
Cas No.:4345-03-3
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
D-α-Tocopherol Succinate (Vitamin E succinate) is an antioxidant tocopherol and a salt form of vitamin E. D-α-Tocopherol Succinate inhibits Cisplatin -induced cytotoxicity. D-α-Tocopherol Succinate can be used for the research of cancer[1][2].
D-α-Tocopherol Succinate (1-20 μM; 24 hours) shows cytotoxicity to HEI-OC1 cells[1].
D-α-Tocopherol Succinate (10 μM; 48 hours) protects HEI-OC1 cells against cisplatin-induced ototoxicity and inhibits caspase-3 activity[1].
D-α-Tocopherol Succinate (0-50 μM; 18 hours) shows cytotoxicity to TC-1 tumor cells[2].
Cell Cytotoxicity Assay[1]
| Cell Line: | HEI-OC1 cell line |
| Concentration: | 1-20 μM |
| Incubation Time: | 24 hours |
| Result: | Significantly induced cytotoxicity at a concentration of 20 μM and showed a higher cytotoxicity potency compared with 10 μM. |
Cell Viability Assay[1]
| Cell Line: | HEI-OC1 cell line |
| Concentration: | 10 μM |
| Incubation Time: | 48 hours |
| Result: | Increased cisplatin-induced cell population. Inhibited cisplatin-induced necrotic, ROS production and late apoptosis. Decreased cleaved PARP and inhibited the expression of caspase-3 which related to cisplatin-induced apoptosis. |
Cell Cytotoxicity Assay[2]
| Cell Line: | TC-1 tumor cells |
| Concentration: | 0, 25 and 50 μM |
| Incubation Time: | 18 hours |
| Result: | Dose-dependently showed cytotoxic and induced a higher percentage of necrotic TC-1 cells as opposed to apoptotic cells. |
D-α-Tocopherol Succinate (1-2 mg/kg; i.p. three times at 2 day intervals from TC-1 tumor cells injection for 10 days to 14 days) shows antitumor effects to mice with TC-1 tumor[2].
| Animal Model: | Six- to eight-week-old female C57BL/6 mice with TC-1 tumor cells[2] |
| Dosage: | 1 and 2 mg/kg |
| Administration: | Intraperitoneal injection; 1 and 2 mg/kg three times at 2 day intervals; from TC-1 tumor cells injection for 10 days to 14 days |
| Result: | Dreased the tumor volume, especially with a dose of 2 mg/kg. |
[1]. Kim SK, et al. The effects of the antioxidant α-tocopherol succinate on cisplatin-induced ototoxicity in HEI-OC1 auditory cells. Int J Pediatr Otorhinolaryngol. 2016 Jul;86:9-14.
[2]. Kang TH, et al. Treatment of tumors with vitamin E suppresses myeloid derived suppressor cells and enhances CD8+ T cell-mediated antitumor effects. PLoS One. 2014 Jul 29;9(7):e103562.
| Cas No. | 4345-03-3 | SDF | Download SDF |
| 别名 | Vitamin E succinate | ||
| 分子式 | C33H54O5 | 分子量 | 530.78 |
| 溶解度 | 储存条件 | Store at -20°C | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.884 mL | 9.4201 mL | 18.8402 mL |
| 5 mM | 0.3768 mL | 1.884 mL | 3.768 mL |
| 10 mM | 0.1884 mL | 0.942 mL | 1.884 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。