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Cy7.5 NHS ester (non-sulfonated)

目录号 : GC14305

Labeling of amino-groups in biomolecules.

Cy7.5 NHS ester (non-sulfonated) Chemical Structure

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5mg
¥5,999.00
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Sample solution is provided at 25 µL, 10mM.

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产品描述

Cy7.5 NHS ester is a NIR dye with low aqueous solubility and long-wave emission. This labeling reagent is amine-reactive derivative of this fluorophore. In vivo, near infrared radiation can be used for imaging applications for it readily penetrates tissues. This product can be used for the labeling of various biomolecules such as proteins and peptides which contain amine groups, to track their distribution in organism in vivo. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.

In mice, Cy7.5 NHS ester labeled insulin (Cy7.5-INS) and were injected via subcutaneous injection at insulin dose of 1 IU/kg. The biodistribution of Cy7.5-Labeled insulin, INS/HTCC, and INS/HTCC-CA11 Nanoparticles was easily analyzed [1].

Reference:
[1] Zhang, Z. ; Cai, H.; Liu, Z.; Yao, P. Effective Enhancement of Hypoglycemic Effect of Insulin by Liver-targeted Nanoparticles Containing Cholic Acid-modified Chitosan Derivative. Molecular Pharmaceutics, 2016, 13(7), 2433-2442.

Chemical Properties

Cas No. SDF
化学名 3-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-1,1-dimethyl-2-((E)-2-((E)-3-((E)-2-(1,1,3-trimethyl-1H-benzo[e]indol-2(3H)-ylidene)ethylidene)cyclohex-1-en-1-yl)vinyl)-1H-benzo[e]indol-3-ium
Canonical SMILES CC1(C)C2=C3C(C=CC=C3)=CC=C2[N+](CCCCCC(ON4C(CCC4=O)=O)=O)=C1/C=C/C5=C/C(CCC5)=C/C=C6C(C)(C)C7=C(C=CC=C8)C8=CC=C7N\6C
分子式 C49H52ClN3O4 分子量 782.41
溶解度 soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water 储存条件 Please store the product under the recommended conditions in the Certificate of Analysis.
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

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1 mg 5 mg 10 mg
1 mM 1.2781 mL 6.3905 mL 12.781 mL
5 mM 0.2556 mL 1.2781 mL 2.5562 mL
10 mM 0.1278 mL 0.6391 mL 1.2781 mL
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第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
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