Cyanine Dyes(花青染料)
Cyanine dyes are molecules containing polymethine bridge between two nitrogen atoms with a delocalized charge.
Due to their structure, cyanines have outstandingly high extinction coefficients often exceeding 100,000 Lmol-1cm-1. Different substituents allow to control properties of the chromophore, such as absorbance wavelength, photostability, and fluorescence. For example, absorbance and fluorescence wavelength can be controlled by a choice of polymethine bridge length: longer cyanines possess higher absorbance and emission wavelengths up to near infrared region.
Cyanine dyes are molecules containing polymethine bridges between two nitrogen atoms with a delocalized charge which have been used for many years as dyes in life science. Since the Cy3, Cy5 and Cy7 were made commercially available as succinimidyl esters in the early 1990s, cyanine dyes began to be used widely as labels for nucleic acids. Because of the good properties of low non-specific binding to biomolecules and bright fluorescence owing to their huge extinction coefficients and good quantum yields, the Cy dyes have been widespread used in DNA and RNA labelling. Cyanine dyes can be divided into two groups based on water solubility: non-sulfonated cyanines and sulfonated cyanines.
Non-sulfonated cyanines
Available non-sulfonated dyes include Cy3, Cy3.5, Cy5, Cy5.5, Cy7, and Cy7.5. Cy stands for 'cyanine', and the first digit identifies the number of carbon atoms between the indolenine groups. The suffix .5 is added for benzo-fused cyanines. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. They are organic co-solvent soluble (5-20% of DMF or DMSO). When these molecules are used for biomolecule labeling, they should be dissolved in organic solvent first, and added to a solution of biomolecule (protein, peptide, amino-labeled DNA) in appropriate aqueous buffer. Fluorescent properties of non-sulfonated cyanines have little dependence on solvent and surrounding.
Sulfonated cyanines
Available sulfonated cyanines include sulfo-Cy3, sulfo-Cy5, and sulfo-Cy7, which have additional sulfo-groups that facilitate dissolution of dye molecules in aqueous phase. Charged sulfonate groups decrease aggregation of dye molecules and heavily labeled conjugates. Sulfonated cyanines are highly water soluble, and they do not use organic co-solvent for the labeling in aqueous environment.
- Cat.No. 产品名称 Information
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GC15113
Cy3 alkyne (non-sulfonated)
used for Click chemistry to label any molecule bearing azide group.
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GC14171
Cy3 azide
used for Click chemistry to label alkyne-modified oligonucleotides
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GC17605
Cy3 azide (non-sulfonated)
used for Click chemistry to label alkyne-modified oligonucleotides
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GC19616
Cy3 Bis carboxylic acid
非反应性荧光团,用于对照实验和校准,溶于水
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GC19614
Cy3 Bis NHS ester
荧光染料,含有两个氨基反应基团,溶于水
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GC17551
Cy3 carboxylic acid (non-sulfonated)
Non-reactive fluorophore, for control experiments, and for calibration
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GC19618
Cy3 carboxylic acid(Et)
非反应性荧光团,用于对照实验和校准 ,溶于水
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GC11356
Cy3 hydrazide (non-sulfonated)
carbonyl-reactive dye
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GC18064
Cy3 maleimide (non-sulfonated)
Labeling of cysteine residues, as well as other thiolated molecules
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GC17345
Cy3 NHS ester
Cy3 NHS 酯 (Cyanine3 NHS 酯) 是一种黄色荧光染料,用于标记生物分子中的氨基。
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GC12618
Cy3 NHS ester (non-sulfonated)
标记生物分子中的氨基。
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GC19612
Cy3 NHS ester(Et)
氨基反应性荧光染料,溶于水
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GC35767
Cy3-N3
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GC35768
CY3-SE
An amine-reactive fluorescent probe
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GC30161
CY3-YNE
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GC19610
Cy3.5 alkyne (non-sulfonated)
用于点击化学反应的染料炔
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GC12923
Cy3.5 azide (non-sulfonated)
used for Click chemistry to label alkyne-modified oligonucleotides
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GC13938
Cy3.5 hydrazide (non-sulfonated)
carbonyl-reactive dye
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GC12024
Cy3.5 maleimide (non-sulfonated)
Labeling of cysteine residues, as well as other thiolated molecules
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GC17971
Cy3.5 NHS ester (non-sulfonated)
标记生物分子中的氨基。
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GC35769
CY5
A cyanine-containing fluorochrome
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GC47136
Cy5 (triethylamine salt)
A cyanine-containing fluorochrome
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GC15007
Cy5 alkyne (non-sulfonated)
used for Click chemistry to label any molecule bearing azide group.
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GC14394
Cy5 amine (non-sulfonated)
can be conjugated with reactive groups such as NHS esters, carboxy groups (after carbodiimide activation), and epoxides.
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GC12452
Cy5 azide
用于点击化学以标记炔烃修饰的寡核苷酸
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GC12480
Cy5 azide (non-sulfonated)
used for Click chemistry to label alkyne-modified oligonucleotides
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GC19617
Cy5 Bis carboxylic acid
非反应性荧光团,用于对照实验和校准,溶于水
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GC19615
Cy5 Bis NHS ester
荧光染料,含有两个氨基反应基团,溶于水
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GC10748
Cy5 Boc-hydrazide (non-sulfonated)
carbonyl-reactive dye
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GC15339
Cy5 carboxylic acid
Non-reactive fluorophore, for control experiments, and for calibration
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GC12471
Cy5 carboxylic acid (non-sulfonated)
Non-reactive fluorophore, for control experiments, and for calibration
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GC19619
Cy5 carboxylic acid(Et)
非反应性荧光团,用于对照实验和校准 ,溶于水
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GC15809
Cy5 hydrazide (non-sulfonated)
carbonyl-reactive dye
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GC18124
Cy5 maleimide (non-sulfonated)
Labeling of cysteine residues, as well as other thiolated molecules
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GC12907
Cy5 NHS ester
Cy5 NHS ester(Cyine5-NHS)是一种红色荧光染料,用于标记肽、蛋白质和寡核苷酸中的氨基。
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GC10047
Cy5 NHS ester (non-sulfonated)
标记生物分子中的氨基。
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GC19613
Cy5 NHS ester(Et)
氨基反应性荧光染料,溶于水
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GC30247
CY5-N3
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GC61630
Cy5-PEG3-azide
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GC35771
CY5-SE
An amine-reactive fluorescent probe
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GC47137
Cy5-SE (triethylamine salt)
An amine-reactive fluorescent probe
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GC30117
CY5-YNE
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GC39818
Cy5.5
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GC19611
Cy5.5 alkyne (non-sulfonated)
远红外/近红外染料,用于点击化学标记
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GC14684
Cy5.5 azide (non-sulfonated)
used for Click chemistry to label alkyne-modified oligonucleotides
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GC16132
Cy5.5 carboxylic acid (non-sulfonated)
Non-reactive fluorophore, for control experiments, and for calibration
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GC12343
Cy5.5 hydrazide (non-sulfonated)
carbonyl-reactive dye
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GC11653
Cy5.5 NHS ester (non-sulfonated)
Cy5.5-NHS酯是一种近红外胺活性染料,用于标记肽、蛋白质和寡核苷酸的氨基。
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GC35770
Cy5.5-SE
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GC35772
CY7
A cyanine-containing fluorochrome