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Cy5 carboxylic acid (non-sulfonated) Sale

目录号 : GC12471

Non-reactive fluorophore, for control experiments, and for calibration

Cy5 carboxylic acid (non-sulfonated) Chemical Structure

规格 价格 库存 购买数量
5mg
¥1,554.00
现货
25mg
¥2,961.00
现货

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Sample solution is provided at 25 µL, 10mM.

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产品描述

Cy5 carboxylic acid is a non-activated carboxylic acid with low aqueous solubility. For coupling with amines, or protein labeling, Cy5 NHS ester and water-soluble sulfo-Cy5 NHS ester is preferred. This labeling dye has limited water solubility. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.

In the cells, The Cy5 carboxylic acid label on the dye can track the efficiency of the protein expression [1].

Reference:
[1] Deyle, K. M.; Farrow, B.; Hee, Y.Q.; Work, J.; Wong, M.; Lai, B.; Umeda, A.; Millward, S.W.; Nag, A.; Das, S.; Heath, J.R. A protein-targeting strategy used to develop a selective inhibitor of the E17K point mutation in the PH domain of Akt1. Nature Chemistry,2015, 7, 455–462.

Chemical Properties

Cas No. SDF
化学名 6-[(2E)-3,3-dimethyl-2-[(2E,4E)-5-(1,3,3-trimethylindol-1-ium-2-yl)penta-2,4-dienylidene]indol-1-yl]hexanoic acid
Canonical SMILES CC1(C2=CC=CC=C2[N+](=C1C=CC=CC=C3C(C4=CC=CC=C4N3CCCCCC(=O)O)(C)C)C)C
分子式 C32H39ClN2O2 分子量 519.12
溶解度 ≥ 51.9mg/mL in DMSO, ≥ 89 mg/mL in EtOH 储存条件 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

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1 mg 5 mg 10 mg
1 mM 1.9263 mL 9.6317 mL 19.2634 mL
5 mM 0.3853 mL 1.9263 mL 3.8527 mL
10 mM 0.1926 mL 0.9632 mL 1.9263 mL
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