Cy5.5 azide (non-sulfonated)
目录号 : GC14684
used for Click chemistry to label alkyne-modified oligonucleotides
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
This Cy5.5 is a labeling reagent that ready for the use in Click Chemistry reaction which available as 10 mM solution in DMSO. As Cy5.5 azide is a far-red emitting dye, it can replace Alexa Fluor 680, and DyLight 680. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.
In human glioblastoma U87MG cells, to assess the capability of the Cu(I)/M-dots for cell labeling Cy5.5-azide was in combination with Cu(I)/M-dots for imaging of the integrin αⅤβ3 receptor [1].
Reference:
[1] Sun, Y. ; Hong, S.; Ma, X.; Cheng, K.; Wang, J.; Zhang, Z.; Yang, M.; Jiang, Y.; Hong, X.; Cheng, Z. Recyclable Cu(I)/Melanin Dots for Cycloadditions, Bioconjugation and Cell Labeling. Chemical Science.
| Cas No. | SDF | ||
| 化学名 | 3-(6-((3-azidopropyl)amino)-6-oxohexyl)-1,1-dimethyl-2-((1E,3E,5E)-5-(1,1,3-trimethyl-1H-benzo[e]indol-2(3H)-ylidene)penta-1,3-dien-1-yl)-1H-benzo[e]indol-3-ium chloride | ||
| Canonical SMILES | CC1(C)C2=C3C(C=CC=C3)=CC=C2[N+](CCCCCC(NCCCN=[N+]=[N-])=O)=C1/C=C/C=C/C=C4C(C)(C)C5=C(C=CC=C6)C6=CC=C5N\4C.[Cl-] | ||
| 分子式 | C43H49ClN6O | 分子量 | 701.34 |
| 溶解度 | ≥ 70.1mg/mL in DMSO, ≥ 46.2 mg/mL in EtOH with ultrasonic | 储存条件 | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.4258 mL | 7.1292 mL | 14.2584 mL |
| 5 mM | 0.2852 mL | 1.4258 mL | 2.8517 mL |
| 10 mM | 0.1426 mL | 0.7129 mL | 1.4258 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。