Chondroitin sulfate (from bovine)
(Synonyms: 硫酸软骨素; Chondroitin polysulfate sulfate (from bovine)) 目录号 : GN10342
Chondroitin sulfate (from bovine)是从牛软骨中提取的天然多糖。
Cas No.:9007-28-7
Sample solution is provided at 25 µL, 10mM.
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Chondroitin sulfate (from bovine) is a natural polysaccharide extracted from bovine cartilage [1]. Chondroitin sulfate promotes the repair and regeneration of the cartilage matrix, inhibits the activity of cartilage-degrading enzymes, reduces inflammation, and enhances the lubrication of synovial fluid, thereby slowing the degeneration of articular cartilage [2-3]. Chondroitin sulfate is primarily used to treat osteoarthritis [4].
In human peripheral blood mononuclear cells (PBMCs), Chondroitin sulfate (1μg/mL-1mg/mL; 10d) continuously inhibits osteoclast activity at concentrations as low as 1μg/mL [5]. In rabbit palatal fibroblasts, Treatment with Chondroitin sulfate (0-1000ng/mL; 8h) resulted in a dose-dependent increase in cell adhesion and cell proliferation [6].
In chlorhexidine gluconate (CG)-induced peritoneal fibrosis mice model, Chondroitin sulfate (500mg/kg; po; 21d) prevents peritoneal fibrosis in mice by suppressing NF-κB activation [7]. In BALB/c mice, Chondroitin sulfate (400mg/kg; po; 4 weeks) upregulates the differentiation of regulatory T cells, thereby downregulating Th2 responses, inhibiting the production of specific IgE and antigen-induced allergic reactions [8].
References:
[1]. Lamari F N, Karamanos N K. Structure of chondroitin sulfate[J]. Advances in pharmacology, 2006, 53: 33-48.
[2]. Ronca F, Palmieri L, Panicucci P, et al. Anti-inflammatory activity of chondroitin sulfate[J]. Osteoarthritis and Cartilage, 1998, 6: 14-21.
[3]. Iovu M, Dumais G, Du Souich P. Anti-inflammatory activity of chondroitin sulfate[J]. Osteoarthritis and Cartilage, 2008, 16: S14-S18.
[4]. Henrotin Y, Mathy M, Sanchez C, et al. Chondroitin sulfate in the treatment of osteoarthritis: from in vitro studies to clinical recommendations[J]. Therapeutic advances in musculoskeletal disease, 2010, 2(6): 335-348.
[5]. Cantley M D, Rainsford K D, Haynes D R. Comparison of the ability of chondroitin sulfate derived from bovine, fish and pigs to suppress human osteoclast activity in vitro[J]. Inflammopharmacology, 2013, 21(6): 407-412.
[6]. Zou X H, Foong W C, Cao T, et al. Chondroitin sulfate in palatal wound healing[J]. Journal of dental research, 2004, 83(11): 880-885.
[7]. Abe S, Obata Y, Oka S, et al. Chondroitin sulfate prevents peritoneal fibrosis in mice by suppressing NF-κB activation[J]. Medical Molecular Morphology, 2016, 49(3): 144-153.
[8]. Campo G M, Avenoso A, Campo S, et al. Chondroitin-4-sulphate inhibits NF-kB translocation and caspase activation in collagen-induced arthritis in mice[J]. Osteoarthritis and cartilage, 2008, 16(12): 1474-1483.
Chondroitin sulfate (from bovine)是从牛软骨中提取的天然多糖 [1]。Chondroitin sulfate能够促进软骨基质的修复和再生，抑制软骨降解酶的活性，减轻炎症，增强滑液的润滑作用，从而减缓关节软骨的退化 [2-3]。Chondroitin sulfate主要用于治疗骨关节炎 [4]。
在人外周血单核细胞（PBMC）中，Chondroitin sulfate（1μg/mL-1mg/mL；10d）在低至1μg/mL的浓度下即可持续抑制破骨细胞活性 [5]。在兔腭成纤维细胞中，Chondroitin sulfate（0-1000ng/mL；8h）治疗可导致细胞粘附和细胞增殖呈剂量依赖性增加 [6]。
在葡萄糖酸氯己定（CG）诱导的腹膜纤维化小鼠模型中，Chondroitin sulfate（500mg/kg；po；21d）通过抑制NF-κB活化来预防小鼠腹膜纤维化 [7]。在BALB/c小鼠中，Chondroitin sulfate（400mg/kg；po；4 周）上调调节性T细胞的分化，从而下调Th2反应，抑制特异性IgE的产生和抗原诱导的过敏反应 [8]。
| Cell experiment [1]: | |
Cell lines | Human peripheral blood mononuclear cells (PBMCs) |
Preparation Method | Human peripheral blood mononuclear cells (PBMCs) were seeded at 2 × 106 cells per mL on day 0 in a-minimal essential medium supplemented with 10 % fetal calf serum, 1 % penicillin–streptomycin, and 1%L-glutamine with 100nM vitamin D3, 100nM dexamethasone, and 25ng/mL of recombinant human macrophage colony stimulating factor (M-CSF). Cells were maintained for a period of 17 days at 37℃ with 5 % CO2. From day 7 onwards, human recombinant RANKL (50ng/mL) was added to the culture medium. The Chondroitin sulfate compounds derived from different sources were added to the medium from day 7 onwards with concentrations ranging from 1μg/mL to 1mg/mL. Control wells were treated with all the same culture supplements without Chondroitin sulfate compounds. Cells were either grown directly on the cell culture chamber slides to assess osteoclast formation or onto 5mm whale dentine slices for pit resorption analysis. |
Reaction Conditions | 1μg/mL-1mg/mL; 10d |
Applications | Chondroitin sulfate continuously inhibits osteoclast activity at concentrations as low as 1μg/mL. |
| Animal experiment [2]: | |
Animal models | Chlorhexidine gluconate (CG)-induced peritoneal fibrosis mice model |
Preparation Method | In this study, peritoneal fibrosis was induced in mice by intraperitoneal injection of 0.1% chlorhexidine gluconate (CG) every two days for three weeks. Mice were randomly divided into four groups: the control and Chondroitin sulfate groups received intraperitoneal injection of 15% ethanol saline alone and oral administration of water or Chondroitin sulfate; the CG and CG + Chondroitin sulfate groups received intraperitoneal injection of 0.1% CG and oral administration of water or Chondroitin sulfate. Chondroitin sulfate was administered daily via an orogastric tube. After 21 days, the mice were sacrificed, and peritoneal tissue was obtained for morphological analysis using Masson's trichrome staining to assess the extent of peritoneal fibrosis. |
Dosage form | 500mg/kg; po; 21d |
Applications | Chondroitin sulfate prevents peritoneal fibrosis in mice by suppressing NF-κB activation. |
References: | |
| Cas No. | 9007-28-7 | SDF | |
| 别名 | 硫酸软骨素; Chondroitin polysulfate sulfate (from bovine) | ||
| 分子式 | (C14H21NO14S)n | 分子量 | N/A |
| 溶解度 | ≥ 154.8mg/mL in Water | 储存条件 | Store at 2-8°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >95.00%
- COA (Certificate Of Analysis)
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