Calcitonin salmon (Salmon calcitonin)
(Synonyms: 鲑降钙素,Salmon calcitonin) 目录号 : GC32851
Calcitonin salmon (Salmon calcitonin)是一种从鲑鱼中提取的降钙素类药物。
Cas No.:47931-85-1
Sample solution is provided at 25 µL, 10mM.
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Calcitonin salmon (Salmon calcitonin) is a calcitonin drug derived from salmon [1]. Calcitonin salmon is used to treat bone metabolism disorders and has the effects of inhibiting bone resorption and lowering blood calcium [2-3].
In Articular cartilage explants, Calcitonin salmon (0.1-100nM; 16d) stimulates proteoglycan synthesis in human articular cartilage explants [4]. In C6 cells, all concentrations of Calcitonin salmon (3µg/mL, 6µg/mL, 12µg/mL, 25µg/mL, 50µg/mL; 1h) significantly enhanced cell viability after glutamate-induced cytotoxicity [5]. In HBV-163 cells, Calcitonin salmon (0.03-3mU/mL; 48h) increases the concentration of insulin-like growth factors in serum-free cultures of human osteoblast-line cells [6]. In SH-SY5Y cells, treatment with Calcitonin salmon (50nM, 100nM; 24h) inhibit cell viability [7].
In male mice, acute Calcitonin salmon (5μg/kg; ip; single injection) administration attenuates nicotine-induced locomotor stimulation and dopamine release in the NAc shell [8]. In animal model of type 2 diabetes, oral administration of Calcitonin salmon (0.5mg/kg, 1mg/kg, 2mg/kg; po; 10 weeks) attenuated the development of hyperglycemia in severely diabetic zucker diabetic fatty rats in a dose-dependent manner [1]. In CD1 male mice, Calcitonin salmon (2.5IU/kg; ip; single injection) can enhance the analgesic effect of antidepressants that reduce 5-HT uptake [9]. In male albino mice CD1, injection of Calcitonin salmon (40IU/kg; ip; single injection) induces analgesia in mice [10].
References:
[1]. Feigh M, Andreassen KV, Neutzsky‐Wulff AV, et al. Oral salmon calcitonin attenuates hyperglycaemia and preserves pancreatic beta‐cell area and function in Zucker diabetic fatty rats. British journal of pharmacology. 2012 Sep; 167(1): 151-163.
[2]. Chesnut C3, Azria M, Silverman S, et al. Salmon calcitonin: a review of current and future therapeutic indications. Osteoporosis international. 2008 Apr; 19: 479-491.
[3]. Wisneski LA. Salmon calcitonin in the acute management of hypercalcemia. Calcified tissue international. 1990 Jan; 46: S26-30.
[4]. Sondergaard BC, Madsen SH, Segovia-Silvestre T, et al. Investigation of the direct effects of salmon calcitonin on human osteoarthritic chondrocytes. BMC musculoskeletal disorders. 2010 Dec; 11: 1-10.
[5]. Taskiran AS, Ergul M. The effect of salmon calcitonin against glutamate-induced cytotoxicity in the C6 cell line and the roles the inflammatory and nitric oxide pathways play. Metabolic Brain Disease. 2021 Oct; 36(7): 1985-1993.
[6]. Farley J, Dimai HP, Stilt-Coffing B, et al. Calcitonin increases the concentration of insulin-like growth factors in serum-free cultures of human osteoblast-line cells. Calcified tissue international. 2000 Sep; 67: 247-254.
[7]. Varol I, Karabork S, Cetinkaya A. Anti-proliferative effects of salmon calcitonin on SH-SY5Y neuroblastoma in vitro. Experimental Biomedical Research. 2023; 6(1): 6.
[8]. Aranäs C, Vestlund J, Witley S, et al. Salmon calcitonin attenuates some behavioural responses to nicotine in male mice. Frontiers in Pharmacology. 2021 Jun 21; 12: 685631.
[9]. Ormazábal MJ, Goicoechea C, Sánchez E, et al. Salmon calcitonin potentiates the analgesia induced by antidepressants. Pharmacology Biochemistry and Behavior. 2001 Jan 1; 68(1): 125-133.
[10]. Colado MI, Ormazabal MJ, Goicoechea C, et al. Involvement of central serotonergic pathways in analgesia elicited by salmon calcitonin in the mouse. European journal of pharmacology. 1994 Feb 11;252(3): 291-297.
Calcitonin salmon (Salmon calcitonin)是一种从鲑鱼中提取的降钙素类药物 [1]。Calcitonin salmon用于治疗骨代谢紊乱，具有抑制骨吸收、降低血钙的作用 [2-3]。
在关节软骨外植体中，Calcitonin salmon（0.1-100nM；16d）可刺激人关节软骨外植体中的蛋白多糖合成 [4]。在C6细胞中，所有浓度的鲑鱼降钙素（3µg/mL、6µg/mL、12µg/mL、25µg/mL、50µg/mL；1h）均显著增强谷氨酸诱导的细胞毒性后的细胞活力 [5]。在HBV-163细胞中，Calcitonin salmon（0.03-3mU/mL；48h）可增加人成骨细胞无血清培养物中胰岛素样生长因子的浓度 [6]。在SH-SY5Y细胞中，Calcitonin salmon（50nM、100nM；24h）处理可抑制细胞活力 [7]。
在雄性小鼠中，急性给予Calcitonin salmon（5μg/kg；ip；单次注射）可减弱尼古丁诱导的运动刺激和NAc壳中的多巴胺释放 [8]。在2型糖尿病动物模型中，口服Calcitonin salmon（0.5mg/kg、1mg/kg、2mg/kg；po；10周）可剂量依赖性地减缓重度糖尿病Zucker型糖尿病肥胖大鼠的高血糖发展 [1]。在CD1雄性小鼠中，Calcitonin salmon（2.5IU/kg；ip；单次注射）可增强降低5-HT摄取的抗抑郁药的镇痛作用 [9]。在CD1雄性白化小鼠中，注射鲑鱼降钙素（40IU/kg；ip；单次注射）可诱导小鼠镇痛 [10]。
| Cell experiment [1]: | |
Cell lines | SH-SY5Y cells |
Preparation Method | After reaching the appropriate confluence, the cells were passaged and 100μL equal volumes of Calcitonin salmon (Salmon calcitonin) concentrations prepared to be 50 and 100nM) were given to the cells and used defined times incubated. Cells were incubated in a humid environment at 37℃ in a 5% CO2 air mixture. |
Reaction Conditions | 50nM, 100nM; 24h |
Applications | Treatment with Calcitonin salmon (Salmon calcitonin) inhibit cell viability. |
| Animal experiment [2]: | |
Animal models | Subcutaneous HCC tumor model |
Preparation Method | Nicotine ditartrate was diluted in vehicle solution (sodium hydroxide was added until pH = 7). The dose of nicotine (0.5mg/kg, IP) was calculated from the salt form (i.e., 1.081μmol/kg of nicotine ditartrate). The dose was selected as it activates the mesolimbic dopamine system, as seen by enhanced locomotion, accumbal dopamine release and CPP in male mice. A low dose of nicotine (0.125mg/kg, IP) was used on Day 8 of the locomotor sensitisation experiment. This was done to establish that mice previously treated (Day 1–5) with nicotine show locomotor response to a low dose of nicotine, whereas vehicle-treated mice do not respond similarly. Nicotine was injected 15min prior to behavioural testing. Calcitonin salmon (Salmon calcitonin) was diluted in vehicle (0.9%, NaCl solution) and was administered intraperitoneally (IP) at the dose of 5μg/kg, 30min prior to nicotine administration. |
Dosage form | 5μg/kg; ip; single injection |
Applications | Acute Calcitonin salmon (Salmon calcitonin) administration attenuates nicotine-induced locomotor stimulation and dopamine release in the NAc shell in male mice. |
References: | |
| Cas No. | 47931-85-1 | SDF | |
| 别名 | 鲑降钙素,Salmon calcitonin | ||
| Canonical SMILES | Cys-Ser-Asn-Leu-Ser-Thr-Cys-Val-Leu-Gly-Lys-Leu-Ser-Gln-Glu-Leu-His-Lys-Leu-Gln-Thr-Tyr-Pro-Arg-Thr-Asn-Thr-Gly-Ser-Gly-Thr-Pro-NH2 (Disulfide bridge: Cys1-Cys7) | ||
| 分子式 | C145H240N44O48S2 | 分子量 | 3431.85 |
| 溶解度 | Water : ≥ 50 mg/mL (14.57 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.2914 mL | 1.4569 mL | 2.9139 mL |
| 5 mM | 0.0583 mL | 0.2914 mL | 0.5828 mL |
| 10 mM | 0.0291 mL | 0.1457 mL | 0.2914 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.50%
- COA (Certificate Of Analysis)
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