C12E8
(Synonyms: 辛乙烯二醇单正十二烷基酯,C12E8;八甘醇单十二醚) 目录号 : GC43020
C12E8是一种非离子表面活性剂，全名为表面活性剂聚氧乙二醇，属于聚氧乙烯醚类表面活性剂。
Cas No.:3055-98-9
Sample solution is provided at 25 µL, 10mM.
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C12E8 is a non-ionic surfactant, its full name is surfactant polyoxyethylene glycol, it belongs to the polyoxyethylene ether surfactant [1]. C12E8 regulates the curvature and morphological transition of phospholipid vesicle membrane in a concentration-dependent manner, promoting natural morphological changes at low concentrations and inducing abnormal deformation and ultimately leading to vesicle disintegration at high concentrations [2-3].
In DC3F cells, C12E8 (0.05mg/mL; 45min) decreases the voltage required for irreversible electroporation in planar lipid bilayers [4]. In erythrocytes, large flat invaginations/endovesicles with a compressed central region and a globular periphery were found in sections of erythrocytes treated with C12E8 (44μM; 60 min) [5].
References:
[1]. Ruiz CC, Molina-Bolívar JA. Characterization of mixed non-ionic surfactants n-octyl-β-d-thioglucoside and octaethylene–glycol monododecyl ether: Micellization and microstructure. Journal of colloid and interface science. 2011 Sep 1; 361(1): 178-185.
[2]. Mavcic B, Babnik B, Iglic A, et al. Shape transformation of giant phospholipid vesicles at high concentrations of C12E8. Bioelectrochemistry. 2004 Jun 1; 63(1-2): 183-187.
[3]. Guo Y. Surface interactions between poly (ethylene glycol) and phospholipid bilayers: Effects on aggregation and fusion. State University of New York at Buffalo; 1993.
[4]. Kanduser M, Fosnaric M, Sentjurc M, et al. Effect of surfactant polyoxyethylene glycol (C12E8) on electroporation of cell line DC3F. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2003 Mar 12; 214(1-3): 205-217.
[5]. Bobrowska-Hägerstrand M, Kralj-Iglič V, Iglič A, et al. Torocyte membrane endovesicles induced by octaethyleneglycol dodecylether in human erythrocytes. Biophysical journal. 1999 Dec 1; 77(6): 3356-3362.
C12E8是一种非离子表面活性剂，全名为表面活性剂聚氧乙二醇，属于聚氧乙烯醚类表面活性剂 [1]。C12E8以浓度依赖性方式调节磷脂囊泡膜的曲率和形态转变，低浓度时促进囊泡膜的自然形态变化，高浓度时诱导囊泡膜异常变形，最终导致囊泡崩解 [2-3]。
在DC3F细胞中，C12E8（0.05 mg/mL；45分钟）降低了平面脂质双层膜中不可逆电穿孔所需的电压 [4]。在红细胞中，用C12E8（44μM；60分钟）处理的红细胞切片发现大的扁平内陷/内囊泡，具有压缩的中心区域和球状外围 [5]。
| Cell experiment [1]: | |
Cell lines | DC3F cells |
Preparation Method | The cells in suspension were incubated for 45min at 37℃; 0.05mg/mL C12E8 was added to treated cells, while the medium in which C12E8 was dissolved was added to untreated cells. The membrane fluidity was measured by electron paramagnetic resonance method (EPR), using the spin probe methylester of 5-doxyl palmitate (MeFASL), which is lipophilic and therefore incorporates primarily into the membrane lipid bilayer. Sixty milliliters of 0.1mM MeFASL in ethanol solution was placed into the glass tube, then ethanol was evaporated by rotavapor. 1mL of cell suspension that contained 20 × 106 cells was added to the MeFASLfilm formed on the wall of the glass tube, and incubated for 15min while shaking. After that, the cell suspension was centrifuged and the pellet was placed in glass capillary for EPR measurements. From the EPR spectra at 4℃, the maximal hyperfine splitting constant 2AII that reflects the order parameter was measured. |
Reaction Conditions | 0.05mg/mL; 45min |
Applications | C12E8 decreases the voltage required for irreversible electroporation in planar lipid bilayers. |
References: | |
| Cas No. | 3055-98-9 | SDF | |
| 别名 | 辛乙烯二醇单正十二烷基酯,C12E8;八甘醇单十二醚 | ||
| Canonical SMILES | CCCCCCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCO | ||
| 分子式 | C28H58O9 | 分子量 | 538.8 |
| 溶解度 | DMF: 30 mg/ml,DMSO: 15 mg/ml,Ethanol: 30 mg/ml,PBS (pH 7.2): 1 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.856 mL | 9.2799 mL | 18.5598 mL |
| 5 mM | 0.3712 mL | 1.856 mL | 3.712 mL |
| 10 mM | 0.1856 mL | 0.928 mL | 1.856 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet