BRAK Human
目录号 : GP21070BRAK (CXCL14) Human Recombinant
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | C-X-C motif chemokine 14; Small-inducible cytokine B14; Chemokine BRAK; Bolekine; NJAC; KS1; Kec; BMAC; MIP-2g; SCYB14; CXCL14; BRAK; MGC10687. | ||
| Amino Acid Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Lys-Cys-Lys-Cys. | ||
| Solubility | It is recommended to reconstitute the lyophilized CXCL14 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized CXCL14 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution CXCL14 should be stored at 4°C between 2-7 days and for future use below -18°C .Please prevent freeze-thaw cycles. | ||
| Biological Activity | The ED50 of CXCL14 as determined by its ability to induce calcium flux of prostaglandin E2 treated THP1 human acute monocytic leukemia cells was 1.0-10.0 ng/ml. | ||
| Formulation | CXCL14 was lyophilized after extensive dialysis against 20mM Tris-HCl, pH 8.5 and 1M NaCl. | ||
CXCL14 is involved in immunoregulatory and inflammatory processes. BRAK protein is structurally related to the CXC (Cys-X-Cys) subfamily of cytokines. CXCL14 displays chemotactic activity for monocytes but not for lymphocytes, dendritic cells, neutrophils or macrophages. CXCL14 is involved in the homeostasis of monocyte-derived macrophages.
The ED50 of CXCL14 as determined by its ability to induce calcium flux of prostaglandin E2 treated THP1 human acute monocytic leukemia cells was 1.0-10.0 ng/ml.
Lyophilized CXCL14 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution CXCL14 should be stored at 4°C between 2-7 days and for future use below -18°C .Please prevent freeze-thaw cycles.