Blumeatin
(Synonyms: 艾纳香素) 目录号 : GC61743A flavonoid with antioxidant and anticancer activities
Cas No.:118024-26-3
Sample solution is provided at 25 µL, 10mM.
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Blumeatin is a flavonoid that has been found in Blumea and has antioxidant and anticancer activities.1,2,3 It scavenges DPPH radicals in a cell-free assay with a 50% scavenging capacity (SC50) value of 90.8 ?g/ml.1 Blumeatin (10 and 100 ?M) decreases carbon tetrachloride-induced lipid peroxidation in isolated rhesus monkey hepatocytes.2 It is also cytotoxic to KB oral carcinoma cells (IC50 = 47.72 ?g/ml).3
1.Nessa, F., Ismail, Z., Mohamed, N., et al.Free radical-scavenging activity of organic extracts and of pure flavonoids of Blumea balsamifera DC leavesFood Chem.88(2)243-252(2004) 2.Zhao, J.H., Xu, S.-B., Wang, Z.-L., et al.Protective actions of blumea flavanones on primary cultured hepatocytes and liver subcellular organelle against lipid peroxidationJ. Chin. Pharma. Sci.7(3)152-156(1998) 3.Saewan, N., Koysomboon, S., and Chantrapromma, K.Anti-tyrosinase and anti-cancer activities of flavonoids from Blumea balsamifera DCJ. Med. Plant Res.5(6)1018-1025(2011)
Cas No. | 118024-26-3 | SDF | |
别名 | 艾纳香素 | ||
Canonical SMILES | O=C1C[C@@H](C2=CC(O)=CC(O)=C2)OC3=CC(OC)=CC(O)=C13 | ||
分子式 | C16H14O6 | 分子量 | 302.28 |
溶解度 | DMSO: 250 mg/mL (827.05 mM) | 储存条件 | Store at -20°C |
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1 mg | 5 mg | 10 mg | |
1 mM | 3.3082 mL | 16.541 mL | 33.0819 mL |
5 mM | 0.6616 mL | 3.3082 mL | 6.6164 mL |
10 mM | 0.3308 mL | 1.6541 mL | 3.3082 mL |
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[Protective action of Blumeatin against experimental liver injuries]
Zhongguo Yao Li Xue Bao 1993 Jul;14(4):376-8.PMID:8249641doi
Blumeatin (Blu, 5,3',5'-trihydroxy-7-methoxy-dihydro-flavone) was first isolated from Blumea balsamifera DC by Department of Chemistry, Sunyatsen University of China. Blu ip inhibited the increase of serum alanine aminotransferase (AAT) and liver triglyceride and increased serum triglyceride, beta-lipoprotein, and liver glycogen content in CCl4-intoxicated rats. Histological lesions of liver were less severe than those of hepatic injury control. Blu ip 0.65 and 3.25 mg.kg-1 inhibited the increase of serum AAT and hepatic TG in thioacetamide (TAA)-intoxicated mice. Blu ip shortened the pentobarbital sleeping time in CCl4-intoxicated mice. It suggested that Blu could protect liver against injury induced by CCl4 and TAA.
Detailed studies on the anticancer action of rosmarinic acid in human Hep-G2 liver carcinoma cells: evaluating its effects on cellular apoptosis, caspase activation and suppression of cell migration and invasion
J BUON 2020 Jul-Aug;25(4):2011-2016.PMID:33099946doi
Purpose: Oral cancer is one among the devastating types of malignancies and imposes tremendous disease burden on humans. This study was undertaken to investigate the anticancer properties of a plant-derived flavanone, Blumeatin, against human oral cancer cells. Additionally, this study also attempted to unreveal the molecular mechanisms responsible for the anticancer properties of this molecule. Methods: MTT assay was used for the assessment of cell viability. Transwell and wound healing assays were used for the determination of cell invasion and migration, respectively. Comet assay was used for the determination of cell viability. Transmission electron microscopy (TEM) analysis was done to assess the induction of autophagy. The protein expression was determined by western blot analysis. Results: Blumeatin inhibited the growth of SCC-4 oral cancer cells with minimal cytotoxic effects against the normal hTRET-OME cells. The flow cytometric analysis showed that Blumeatin triggers DNA damage in the SCC-4 cells. Blumeatin also activated autophagy in SCC-4 cells which was accompanied with upregulation of LC3B and Beclin 1. This molecule also increased ROS and decreased the MMP levels in human SCC-4 cells. The effects of Blumeatin were also examined on the migration and invasion of the SCC-4 cells and it was revealed that the molecule suppresses both migration and invasion of the SCC-4 oral cancer cells. Conclusion: This study indicates that Blumeatin exhibits potent anticancer effects and points towards its use in the development of a new systemic therapy for oral cancer.
Isoprenylated flavonoid and adipogenesis-promoting constituents of Dodonaea viscosa
J Nat Prod 2012 Apr 27;75(4):699-706.PMID:22512738DOI:10.1021/np2009797.
Ten new isoprenylated flavonol derivatives, dodoviscins A-J (1-10), and seven known compounds (11-17) were isolated from the aerial parts of Dodonaea viscosa. Compounds 1, 2, 4, 5, 7-9, 5,7,4'-trihydroxy-3',5'-bis(3-methyl-2-buten-1-yl)-3-methoxyflavone (11), 5,7,4'-trihydroxy-3',5'-bis(3-methyl-2-buten-1-yl)-3,6-dimethoxyflavone (12), 5,7,4'-trihydroxy-3'-(4-hydroxy-3-methylbutyl)-5'-(3-methyl-2-buten-1-yl)-3,6-dimethyoxyflavone (13), sakuranetin (14), and Blumeatin (15) promoted adipocyte differentiation as characterized by increased triglyceride levels in 3T3L1 cells. Compounds 1, 13, and 15 also enhanced the accumulation of lipid droplets and induced upregulation of the expression of the adipocyte-specific genes aP2 and GLUT4.
RP-HPLC method for the quantitative analysis of naturally occurring flavonoids in leaves of Blumea balsamifera DC
J Chromatogr Sci 2005 Sep;43(8):416-20.PMID:16212782DOI:10.1093/chromsci/43.8.416.
A selective and sensitive reversed-phase (RP) high-performance liquid chromatographic method is developed for the quantitative analysis of five naturally occurring flavonoids of Blumea balsamifera DC, namely dihydroquercetin-7,4'-dimethyl ether (DQDE), Blumeatin (BL), quercetin (QN), 5,7,3',5'-tetrahydroxyflavanone (THFE), and dihydroquercetin-4'-methyl ether (DQME). These compounds have been isolated using various chromatographic methods. The five compounds are completely separated within 35 min using an RP C18, Nucleosil column and with an isocratic methanol-0.5% phosphoric acid (50:50, v/v) mobile phase at the flow rate of 0.9 mL/min. The separation of the compounds is monitored at 285 nm using UV detection. Identifications of specific flavonoids are made by comparing their retention times with those of the standards. Reproducibility of the method is good, with coefficients of variation of 1.48% for DQME, 2.25% for THFE, 2.31% for QN, 2.23% for DQDE, and 1.51% for BL. The average recoveries of pure flavonoids upon addition to lyophilized powder and subsequent extraction are 99.8% for DQME, 99.9% for THFE, 100.0% for BL, 100.6% for DQDE, and 97.4% for QN.
Dual-screening of anti-inflammatory and antioxidant active ingredients of shenxiang suhe pill and its potential multi-target therapy for coronary heart disease
Biomed Pharmacother 2020 Sep;129:110283.PMID:32531677DOI:10.1016/j.biopha.2020.110283.
Background: Shenxiang Suhe Pill (SXSHP), a Chinese medicine formula, is widely used in clinic to treat coronary heart disease (CHD). However, due to the complex composition of SXSHP, its underlying mechanisms and pharmacodynamic properties are still unknown. In this paper, we try to define the compounds of SXSHP by dual-screening the active ingredients with anti-inflammation and antioxidant effects and predict its multi-target-pathway in CHD therapy using network pharmacology. Methods: The chemical constituents in SXSHP were analyzed by UPLC/Q-TOF. Then, the active ingredients with the anti-inflammation and antioxidant effects were dual-screened by in vitro experiments. Ingenuity pathway analysis (IPA) was used to analyze and predict the potential targets and pathways of the anti-inflammatory and antioxidant effects of SXSHP. Results: A total of 38 chemical constituents were identified in SXSHP, among which we screened six anti-inflammatory compounds: luteolin, isorhamnetin-3-O-beta-d-glucoside, 4-hydroxy-3-methoxycinnamaldehyde, benzoic acid, kaempferol-3-O-glucuronide acid, and Blumeatin; and five antioxidant compounds: vanillin, eugenol, muscone, luteolin, and asiatic acid. IPA showed that eugenol, muscone, and 4-hydroxy-3-methoxycinnamaldehyde were closely related to the HIF-1 and IL-15 signaling pathways, which protect against oxidative stress and inflammation, respectively. Conclusions: Among the 38 ingredients in SXSHP, the anti-inflammatory pharmacological effects of isorhamnetin-3-O-beta-d-glucoside, Blumeatin and 4-hydroxy-3-methoxycinnamaldehyde were reported for the first time. According to the network pharmacology analysis, eugenol, 4-hydroxy-3-methoxycinnamaldehyde and muscone are involved in the antioxidant HIF-1 pathway and the anti-inflammatory IL-15 pathway, and that may be the mechanism of SXSHP in the treatment of CHD.