Home>>Signaling Pathways>> Membrane Transporter/Ion Channel>> Sodium Channel>>Benzamil hydrochloride

Benzamil hydrochloride Sale

(Synonyms: 3,5-二氨基-N-[(E)-氨基(苄基氨基)亚甲基]-6-氯-2-吡嗪甲酰胺盐酸盐(1:1),Benzylamiloride hydrochloride) 目录号 : GC11693

An ENaC and NCX inhibitor

Benzamil hydrochloride Chemical Structure

Cas No.:161804-20-2

规格 价格 库存 购买数量
10mg
¥546.00
现货
50mg
¥2,247.00
现货

电话:400-920-5774 Email: sales@glpbio.cn

Customer Reviews

Based on customer reviews.

Sample solution is provided at 25 µL, 10mM.

产品文档

Quality Control & SDS

View current batch:

实验参考方法

Cell experiment:

Individual CCD segments are dissected from rabbit and mouse kidney.The measurements of [Na+]i are used as a reflection of Na+ transport across the apical membrane. EnaC-specific activity is assessed by applying 10−6 M of the ENaC blocker Benzamil hydrochloride, a concentration that does not affect Na+:H+ exchange. Experiments are performed in the presence/absence of luminal Benzamil hydrochloride or the Na:H exchanger blocker HOE694 administers with/without the AT1 receptor blocker candesartan. Final DMSO concentrations are below 0.1% [2].

Animal experiment:

For the acute intracerebroventricular injection in anesthetized rats. A guide cannula is inserted into the right lateral cerebral ventricle, and Benzamil hydrochloride (0.1 nmol/kg, n=6; 1 nmol/kg, n=6; 10 nmol/kg,n=7) or vehicle (n=6) is injected into the right lateral ventricle through a cannula connected to a microsyringe 15 min before the start of intracerebroventricular infusion of hypertonic NaCl. Each injection consists of a volume of 10 μL deliver manually over a period of 30 s. To investigate the effects of Benzamil hydrochloride on the pressor response induced by intracerebroventricular injection of the pressor agent other than hypertonic NaCl, endothelin-1, which has a potent pressor action in the brain of rats, is intracerebroventricularly injected (1 nmol/10 μL) 15 min after the intracerebroventricular preinjection of Benzamil hydrochloride (10 nmol/kg, n=6) or the vehicle (n=6), and mean arterial pressure, heart rate, and abdominal sympathetic nerve firings are recorded for 20 min. Catheters are implanted into both the femoral artery and vein of 12-wk-old male rats anesthetized with urethan. Both catheters are filled with heparinized saline (50 U/mL). Fifteen minutes before the start of intracerebroventricular infusion of hypertonic NaCl, Benzamil hydrochloride (10 nmol/kg, n=6) or vehicle (10 μL, n=6) is injected into a tube connects to the femoral venous catheter by directly inserting the microsyringe, and 0.1 ml of isotonic saline solution is injected through the catheter to deliver the contents into the venous circulation[1].

References:

[1]. Nishimura M, et al. Benzamil blockade of brain Na+ channels averts Na(+)-induced hypertension in rats. Am J Physiol. 1998 Mar;274(3 Pt 2):R635-44.
[2]. Peti-Peterdi J, et al. Angiotensin II directly stimulates ENaC activity in the cortical collecting duct via AT(1) receptors. J Am Soc Nephrol. 2002 May;13(5):1131-5.

产品描述

Benzamil hydrochloride is a specific blocker of sodium channel (ENaC).
The specific ENaC blocker Benzamil hydrochloride adds to the luminal perfusate (10−6 M) significantly decreases baseline [Na+]i by 19.2 mM (n=5) and almost completely inhibits the [NaCl]L-dependent increasing in [Na+]i when coadministers with luminal AngII[2].
Intracerebroventricular injection of endothelin-1 (1 nmol) after intracerebroventricular preinjection of the vehicle of Benzamil hydrochloride markedly increases both mean arterial pressure and abdominal sympathetic nervous activity for 10 min. In SHRSP, intracerebroventricular infusion either of 1 or 10 nmol/kg/day Benzamil hydrochloride decreases systolic blood pressure (4th day: F=6.131, 1 nmol/kg/day, P<0.05, 10 nmol/kg/day, P<0.01; 5th day: F=6.842, 1 nmol/kg/day, P<0.05, 10 nmol/kg/day, P<0.01; 6th day: F=5.988, 1 nmol/kg/day, P<0.05, 10 nmol/kg/day, P<0.01; 7th day: F=7.935, 1 nmol/kg/day, P<0.01, 10 nmol/kg/day, P<0.01), urinary excretion of vasopressin (5th day: F=9.385, 1 nmol/kg/day, P<0.05, 10 nmol/kg/day, P<0.05; 6th day: F=8.783, 1 nmol/kg/day, P<0.01, 10 nmol/kg/day, P<0.01; 7th day: F=8.996, 1 nmol/kg/day, P<0.01, 10 nmol/kg/day, P< 0.01), and norepinephrine (3rd day: F=4.341, 1 nmol/kg/day, P<0.05, 10 nmol/kg/day, P<0.01; 4th day: F=9.865, 1 nmol/kg/day, P<0.01, 10 nmol/kg/day, P<0.01; 5th day: F=14.652, 1 nmol/kg/day, P<0.01, 10 nmol/kg/day, P<0.01; 6th day: F=13.376, 1 nmol/kg/day, P<0.01, 10 nmol/kg/day, P<0.01; 7th day: F=8.594, 1 nmol/kg/day, P<0.01, 10 nmol/kg/day, P<0.01) compare with vehicle[1].
Reference:
[1]. Nishimura M, et al. Benzamil blockade of brain Na+ channels averts Na(+)-induced hypertension in rats. Am J Physiol. 1998 Mar;274(3 Pt 2):R635-44.
[2]. Peti-Peterdi J, et al. Angiotensin II directly stimulates ENaC activity in the cortical collecting duct via AT(1) receptors. J Am Soc Nephrol. 2002 May;13(5):1131-5.

Chemical Properties

Cas No. 161804-20-2 SDF
别名 3,5-二氨基-N-[(E)-氨基(苄基氨基)亚甲基]-6-氯-2-吡嗪甲酰胺盐酸盐(1:1),Benzylamiloride hydrochloride
化学名 (E)-3,5-diamino-N-(N'-benzylcarbamimidoyl)-6-chloropyrazine-2-carboxamide hydrochloride
Canonical SMILES ClC(N=C1C(N/C(N)=N/CC2=CC=CC=C2)=O)=C(N)N=C1N.Cl
分子式 C13H14ClN7O.HCl 分子量 356.21
溶解度 DMSO: 100 mM,Water: 10 mM 储存条件 Store at -20°C
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

制备储备液
1 mg 5 mg 10 mg
1 mM 2.8073 mL 14.0367 mL 28.0733 mL
5 mM 0.5615 mL 2.8073 mL 5.6147 mL
10 mM 0.2807 mL 1.4037 mL 2.8073 mL
  • 摩尔浓度计算器

  • 稀释计算器

  • 分子量计算器

质量
=
浓度
x
体积
x
分子量
 
 
 
*在配置溶液时,请务必参考产品标签上、MSDS / COA(可在Glpbio的产品页面获得)批次特异的分子量使用本工具。

计算

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % saline
计算重置