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Bcl 2 Human (minus BH1 domain)

目录号 : GP22850

B-Cell Leukemia/Lymphoma 2 Human Recombinant (–BH1)

Bcl 2 Human (minus BH1 domain) Chemical Structure

规格 价格 库存 购买数量
2μg
¥840.00
5-10工作日
10μg
¥2,030.00
5-10工作日
1mg
¥67,200.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

产品文档

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产品描述

Bcl-2 Des BH1 domain (136-155 residues) Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containg 198 amino acids 1-135 and 156-218.The Bcl-2 is expressed as His-Tag fusion protein and purified by proprietary chromatographic techniques.

Product Data

Purity Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. Source Escherichia Coli.
Phycical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Shipping Condition Shipped at Room temp.
Synonyms Apoptosis regulator Bcl-2; BCL2; B-cell CLL/lymphoma 2; Bcl-2.
Solubility Suspend Bcl-2 in 100?l of 0.5M Acetic acid, over night at 4°C . Dilute 10 fold into selected buffer system.BCL-2 has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended.
Stability Lyophilized Bcl-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution Bcl-2 should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation The protein contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl.

Introduction

BCL2 gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.

Applications

Input marker or positive control (Western Blotting).Function study.

Stability

Lyophilized Bcl-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution Bcl-2 should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.