AC-green
(Synonyms: VDP-green) 目录号 : GC61592
AC-green(VDP-green)是一种β-烯丙基氨基甲酸酯荧光探针，可选择性地对活体系统中的邻位二硫醇蛋白质(VDPs)进行成像(Λex/Λem=400/475nm)。AC-green可以高灵敏度检测降低牛血清白蛋白(rBSA)。AC-green毒性低，灵敏度高，适用于检测活细胞和斑马鱼中的VDP。
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
AC-green (VDP-green) is a β-allyl carbamate fluorescent probe for specifically imaging vicinal dithiol proteins (VDPs) in living systems (Λex/Λem=400/475 nm). AC-green can detect the reduced bovine serum albumin (rBSA) with high sensitivity. AC-green displays low toxicity and features high sensitivity, and is suitable for sensing VDPs in living cells and zebrafishes[1].
AC-green (VDP-green) can respond to VDPs with more than 60-fold increase of emission in aqueous solution, while there is no significant interference from biological thiols, amino acids or inorganic salts[1]. AC-green (2 μM; 90 min) has no apparent fluorescence signal within the pH range of 5.0-9.0. Addition of rBSA turns on the fluorescence[1]. AC-gree (10 μM) has low cytotoxicity in HepG2 cells and Hela cells[1]. AC-gree (10 μM; for 15 min) images VDPs in living HepG2 cells and bright green fluorescence appeared. This fluorescence is inhibited when the cells are pretreated with PAO, a popular specificligand for protein vicinal dithiols[1]. AC-gree (10 μM; for 20 min) incubates with zebrafishes has a strong fluorescence signal appeared in the green channel[1].
[1]. Lanning Zhao, et al. A β-allyl carbamate fluorescent probe for vicinal dithiol proteins. Chem Commun (Camb). 2020 Mar 5;56(19):2857-2860.
| Cas No. | SDF | ||
| 别名 | VDP-green | ||
| Canonical SMILES | O=C(NC1=CC2=CC=C(C=C2OC1=O)N(CC)CC)C(COC(NC3=CC=CC=C3)=O)=C | ||
| 分子式 | C24H25N3O5 | 分子量 | 435.47 |
| 溶解度 | 储存条件 | Store at -20°C | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.2964 mL | 11.4818 mL | 22.9637 mL |
| 5 mM | 0.4593 mL | 2.2964 mL | 4.5927 mL |
| 10 mM | 0.2296 mL | 1.1482 mL | 2.2964 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
A β-allyl carbamate fluorescent probe for vicinal dithiol proteins
Chem Commun (Camb) 2020 Mar 5;56(19):2857-2860.PMID:32031544DOI:10.1039/c9cc09841k
The first β-allyl carbamate fluorescent probe, AC-green, was reported for specifically imaging vicinal dithiol proteins (VDPs) in living systems. The probe displays low toxicity and features high sensitivity and fast response towards VDPs. A drastic loss of VDPs in a Parkinson's model was disclosed by AC-green, linking the fluctuation of VDP levels to Parkinson's disease for the first time. The structural novelty and favorable properties of AC-green will advance the development of novel VDP probes.