8-Bromo-cGMP, sodium salt
(Synonyms: 8-溴代鸟苷-3',5'-环状单磷酸酯钠盐,??8-bromo-cGMP, 8-bromo Guanosine 3’,5’-cyclic monophosphate) 目录号 : GC10119A cell-permeable cGMP analog
Cas No.:51116-01-9
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
8-bromo-Cyclic GMP (8-bromo-cGMP) is a cell-permeable analog of cGMP that exhibits greater resistance to hydrolysis by phosphodiesterases than its parent compound. It preferentially activates cGMP-dependent protein kinase.[1] At 1 µM-0.1 mM, 8-bromo-cGMP can inhibit acetylcholine-induced increases in intracellular calcium concentrations.[2]
Reference:
[1]. Rashatwar, S.S., Cornwell, T.L., and Lincoln, T.M. Effects of 8-bromo-cGMP on Ca2+ levels in vascular smooth muscle cells: Possible regulation of Ca2+-ATPase by cGMP-dependent protein kinase. Proceedings of the National Academy of Sciences of the United States of America 84(16), 5685-5689 (1987).
[2]. Choi, J., and Farley, J.M. Effects of 8-bromo-cyclic GMP on membrane potential of single swine tracheal smooth muscle cells. Journal of Pharmacology and Experimental Therapeutics 285(2), 588-594 (1998).
Cas No. | 51116-01-9 | SDF | |
别名 | 8-溴代鸟苷-3',5'-环状单磷酸酯钠盐,??8-bromo-cGMP, 8-bromo Guanosine 3’,5’-cyclic monophosphate | ||
化学名 | 8-bromo-guanosine cyclic 3',5'-(hydrogen phosphate), monosodium salt | ||
Canonical SMILES | BrC1=NC2=C(NC(N)=NC2=O)N1[C@@H]3O[C@@H]4[C@H]([C@H]3O)O[P@](OC4)([O-])=O.[Na+] | ||
分子式 | C10H10BrN5NaO7P | 分子量 | 446.09 |
溶解度 | 10mg in PBS(pH7.2) | 储存条件 | Desiccate at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 2.2417 mL | 11.2085 mL | 22.417 mL |
5 mM | 0.4483 mL | 2.2417 mL | 4.4834 mL |
10 mM | 0.2242 mL | 1.1209 mL | 2.2417 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。