5-Hydroxydopamine hydrochloride
(Synonyms: 5-羟基多巴胺盐酸盐) 目录号 : GC30613
5-Hydroxydopamine是人尿液中天然存在的胺。
Cas No.:5720-26-3
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
5-Hydroxydopamine is a naturally occurring amine in human urine.
[1]. Abe K, et al. The effects of 5-hydroxydopamine on salivary flow rates and protein secretion by the submandibular and parotid glands of rats. Exp Physiol. 1996 Jul;81(4):645-53.
| Cas No. | 5720-26-3 | SDF | |
| 别名 | 5-羟基多巴胺盐酸盐 | ||
| Canonical SMILES | OC1=CC(CCN)=CC(O)=C1O.Cl | ||
| 分子式 | C8H12ClNO3 | 分子量 | 205.64 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.8629 mL | 24.3143 mL | 48.6287 mL |
| 5 mM | 0.9726 mL | 4.8629 mL | 9.7257 mL |
| 10 mM | 0.4863 mL | 2.4314 mL | 4.8629 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
5-hydroxydopamine-labeled dopaminergic axons: three-dimensional reconstructions of axons, synapses and postsynaptic targets in rat neostriatum
Neuroscience. 1994 Feb;58(3):593-604.PMID: 8170539DOI:10.1016/0306-4522(94)90084-1
Previous studies employing 5-hydroxydopamine to identify nigrostriatal dopaminergic axons and their synapses found that labeled axons made few synapses or that asymmetric contacts predominated. In contrast, recent studies using tyrosine hydroxylase or dopamine antibody techniques indicate that presumed dopaminergic axons form small symmetric contacts. We re-examined 5-hydroxydopamine-labeled material from the rat neostriatum using serial three-dimensional reconstruction techniques to characterize the morphology of labeled axons, synapses and postsynaptic targets. This ultrastructural analysis revealed a class of heavily labeled axons that are small (0.06-1.5 microns in diameter) and lack large varicosities. These axons form small (0.011-0.09 microns 2), en passant, symmetric synapses, mainly onto dendritic spines and spiny dendritic shafts and, in some cases, onto aspiny dendritic segments near branch points. The sites of these synapses along the axon appeared unrelated to the locations of axonal enlargements, suggesting that counting varicosities may not be an accurate indication of the extent of dopaminergic innervation in the neostriatum. The characteristics of these 5-hydroxydopamine-labeled elements correspond in all respects to axons and synapses identified as dopaminergic by immunohistochemistry in previous studies. In tissue in which all labeled and unlabeled synapses were classified, approximately 9% of all synapses were identified as dopaminergic by this type of label. Three-dimensional reconstructions provided additional insight concerning the interaction of dopaminergic afferents with postsynaptic striatal targets and their relation to other afferents to these neurons. They reveal that a short, unbranched dopaminergic axonal segment can make multiple synapses onto dendritic spines, shafts and branch points of one or more dendrites. In addition, one dendrite can receive contacts from several labeled axons. Dopamine synapses onto spines are always associated with unlabeled, asymmetric synapses onto the same spine. Synapses of various morphologies with a distinctly different, lighter form of labeling were much rarer, and may represent other aminergic afferents to the neostriatum. The presence of this second form of label in earlier 5-hydroxydopamine studies may have contributed to the long-standing controversy over the appearance of dopaminergic synapses examined by different techniques. Our results help to resolve this controversy and confirm that the nigrostriatal projection makes small symmetric synapses with a variety of striatal targets.
Analysis of inhibitory and excitatory actions of dopamine on chemoreceptor discharges of carotid body of cat in vivo
Jpn J Physiol. 1981;31(5):695-704.PMID: 7328918DOI: 10.2170/jjphysiol.31.695
Chemoreceptor discharges were recorded from the carotid sinus nerve of the cat in vivo, and their frequency was used as an index of receptor activity. The effects of dopamine on chemoreceptor activity were analyzed in normal and ischemic carotid bodies. Intra-arterial injections of dopamine (DA) induced various patterns of chemoreceptor responses; simple inhibition, inhibition followed by excitation, and simple excitation, depending upon doses and the time interval between two injections. After a large dose of DA, a previous inhibitory response to DA was converted into an excitatory one. Pretreatment of the animal with reserpine (0.1 mg/kg, i.v.) or 5-hydroxydopamine (5 mg/kg, i.v.) also converted the inhibitory response to DA into the excitatory one. After haloperidol (0.1 mg/kg, i.v.), the inhibitory response to DA was completely blocked, and DA induced a dose-dependent increase in chemoreceptor discharges. After 1-hr ischemia of the carotid body, DA induced only the inhibitory response, which was blocked by haloperidol, but did not produce any excitatory responses. Results indicate that DA exerts a self-blocking action on inhibitory dopaminergic receptors which are possibly located on the nerve ending, and that DA, also acting directly on the glomus cell, would produce the chemoreceptor excitation.
The development of innervation in the rat atrioventricular node
Cell Tissue Res.1977 Mar 1;178(1):73-82.PMID: 837426DOI: 10.1007/BF00232825
The problem of development of the innervation of the rat atrioventricular node has been investigated by electron microscopy. Nerve bundles appear in relation to the node as early as the second postnatal day and vesiculated axons are seen throughout the entire node by the fourth day. Intimate contacts between nodal cells, axons and terminal varicosities are frequently observed. Use of the 5-hydroxydopamine tracer technique has enabled the identification of both cholinergic and adrenergic axons. It is concluded that the node has a dual innervation although cholinergic endings far outnumber those classified as adrenergic on the sixth postnatal day. These results are quite different to earlier findings made at the light microscope level and the discrepancies are discussed with respect to the histochemical techniques used. The suggestion that nodal differentiation is induced by nerves is considered in relation to the differences in cholinesterase activity exhibited by nodal cells during normal development and following neonatal sympathectomy.
Synaptosomes containing large agranular vesicles isolated from developing rat brain
Acta Physiol Scand.1975 Jul;94(3):393-7.PMID: 1101647DOI:10.1111/j.1748-1716.1975.tb05899.x
Using the subcellular fractionation technique the fine structure of the isolated nerve endings (synaptosomes) from the hemispheres and brain stem of the 1-day old and adult rat was examined. In the synaptosomal fractions of the brain of 1-day old rats we observed a new type of nerve endings containing predominantly large agranular vesicles about 1,000 A in diameter. After incubation with alpha-methylnoradrenaline or 5-hydroxydopamine these vesicles remained agranular. It is assumed that the new type of large vesicles represent developing synaptic vesicles.
Synaptic endings and their postsynaptic targets in neostriatum: synaptic specializations revealed from analysis of serial sections
Proc Natl Acad Sci U S A.1980 Nov;77(11):6926-9.PMID: 6935693DOI: 10.1073/pnas.77.11.6926
Neostriatal tissue from rat brain was examined in ribbons of serial sections, using tissue from subjects in which the synaptic marker 5-hydroxydopamine had been injected into the lateral ventricle. A total of 440 synaptic terminals, identified by the presence of a population of vesicles, was studied, including those labeled by 5-hydroxydopamine and all those unlabeled in the surrounding neuropil. Three categories of synaptic profiles innervating neostriatal dendrites could be discerned. One category contained small rounded or slightly pleomorphic vesicles and consisted of a sample of 375 endings, 53 of which exhibited light to heavy label inside the synaptic vesicles. All of these, labeled and unlabeled, showed evidence of membrane specializations and other conventional criteria of synaptic contact, mostly in the form of asymmetric synaptic contacts with the heads of dendritic spines. A second category of 40 terminals, 3 of which were lightly labeled, contained large rounded or slightly pleomorphic vesicles and made predominantly symmetric contacts with the shafts of dendritic spines or spine-free portions of dendritic membrane. None of these synaptic endings appeared to lack synaptic contact with postsynaptic targets in the neostriatum. A third category of 25 terminals, 3 of which were labeled, contained small flattened vesicles, and these endings also invariably made synaptic contact, mostly onto spine-free dendritic membrane, and were characterized by symmetric membrane thickenings at the point of apposition. Our evidence supports the view that dopaminergic and other synaptic terminals in the rat caudate-putamen make synaptic contact with postsynaptic targets in the neostriatum and, at least in the adult, do not exist in appreciable numbers in the form of terminals that are not apposed to membranes of postsynaptic targets in the neostriatum.