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3-Methoxytyrosine Sale

(Synonyms: 2-氨基-3-(4-羟基-3-甲氧基苯基)丙酸) 目录号 : GC49869

An active metabolite of L-DOPA

3-Methoxytyrosine Chemical Structure

Cas No.:7636-26-2

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25 mg
¥936.00
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50 mg
¥1,233.00
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100 mg
¥1,817.00
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250 mg
¥3,432.00
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产品描述

3-Methoxytyrosine is an active metabolite of L-DOPA .1 It is formed from L-DOPA via O-methylation by catechol-O-methyltransferase (COMT). 3-Methoxytyrosine (10 and 100 µM) inhibits L-DOPA-induced neuroprotection in primary rat mesencephalic dopaminergic neurons.2

1.Miller, J.W., Shukitt-Hale, B., Villalobos-Molina, R., et al.Effect of ?-Dopa and the catechol-O-methyltransferase inhibitor Ro 41-0960 on sulfur amino acid metabolites in ratsClin. Neuropharmacol.20(1)55-66(1997) 2.Asanuma, M., and Miyazaki, I.3-O-Methyldopa inhibits astrocyte-mediated dopaminergic neuroprotective effects of ?-DOPABMC Neurosci.17(1)52(2016)

Chemical Properties

Cas No. 7636-26-2 SDF Download SDF
别名 2-氨基-3-(4-羟基-3-甲氧基苯基)丙酸
Canonical SMILES O=C(C(CC1=CC=C(C(OC)=C1)O)N)O
分子式 C10H13NO4 分子量 211.2
溶解度 Acetonitrile: Slightly soluble,Chloroform: Slightly soluble,DMF: Slightly soluble,DMSO: Slightly soluble,PBS (pH 7.2): Slightly soluble,Water: Slightly soluble 储存条件 -20°C
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1 mg 5 mg 10 mg
1 mM 4.7348 mL 23.6742 mL 47.3485 mL
5 mM 0.947 mL 4.7348 mL 9.4697 mL
10 mM 0.4735 mL 2.3674 mL 4.7348 mL
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Research Update

3-Methoxytyrosine as an indicator of dopaminergic manipulation in equine plasma

J Chromatogr B Analyt Technol Biomed Life Sci 2023 Apr 1;1220:123652.PMID:36933516DOI:10.1016/j.jchromb.2023.123652.

The use of catechol-O-methyltransferase inhibitors may mask doping agents, primarily levodopa, administered to racehorses and prolong the stimulating effects of dopaminergic compounds such as dopamine. It is known that 3-methoxytyramine is a metabolite of dopamine and 3-Methoxytyrosine is a metabolite of levodopa thus these compounds are proposed to be potential biomarkers of interest. Previous research established a urinary threshold of 4,000 ng/mL for 3-methoxytyramine to monitor misuse of dopaminergic agents. However, there is no equivalent biomarker in plasma. To address this deficiency a rapid protein precipitation method was developed and validated to isolate target compounds from 100 µL equine plasma. A liquid chromatography-high resolution accurate mass (LC-HRAM) method using an IMTAKT Intrada amino acid column provided quantitative analysis of 3-Methoxytyrosine (3-MTyr) with lower limit of quantification of 5 ng/mL. Reference population profiling (n = 1129) investigated the expected basal concentrations for raceday samples from equine athletes and showed a right-skewed distribution (skewness = 2.39, kurtosis = 10.65) which resulted from large variation (RSD = 71%) within the data. Logarithmic transformation of the data provided a normal distribution (skewness = 0.26, kurtosis = 3.23) resulting in the proposal of a conservative threshold for plasma 3-MTyr of 1,000 ng/mL at a 99.995% confidence level. A 12-horse administration study of Stalevo® (800 mg L-DOPA, 200 mg carbidopa, 1600 mg entacapone) revealed elevated 3-MTyr concentrations for 24-hours post-administration.

L-3,4-dihydroxyphenylalanine (levodopa) lowers central nervous system S-adenosylmethionine concentrations in humans

J Neurol Neurosurg Psychiatry 1990 Jul;53(7):569-72.PMID:2391519DOI:10.1136/jnnp.53.7.569.

To determine whether levodopa reduces the levels of S-adenosylmethionine in the human central nervous system, cerebrospinal fluid (CSF) concentrations of S-adenosylmethionine, methionine, 3-Methoxytyrosine, levodopa and 5-methyltetrahydrofolate were measured in six children with dopamine deficiency before and after treatment. In four, the lack of dopamine was secondary to a reduction in concentration of levodopa and these were treated with levodopa together with a peripheral dopa-decarboxylase inhibitor. In the other two, levodopa in the central nervous system naturally accumulated due to a congenital deficiency of aromatic-L-amino acid decarboxylase and these were treated with pyridoxine (which in this condition lowers central levodopa concentrations). Raising levodopa concentrations in the central nervous system caused a fall in CSF S-adenosyl-methionine concentration and a rise in CSF 3-Methoxytyrosine concentration. No change was observed in CSF methionine concentration and in all patients CSF 5-methyltetrahydrofolate concentration was normal. With one exclusion there was a linear relationship between CSF S-adenosylmethionine and 3-Methoxytyrosine concentrations. This is the first demonstration of such effects in humans and the implications upon levodopa therapy are discussed.

Explore the Therapeutic Composition and Mechanism of Schisandra chinensis-Acorus tatarinowii Schott on Alzheimer's Disease by Using an Integrated Approach on Chemical Profile, Network Pharmacology, and UPLC-QTOF/MS-Based Metabolomics Analysis

Oxid Med Cell Longev 2022 Jul 11;2022:6362617.PMID:35860432DOI:10.1155/2022/6362617.

Background: Alzheimer's disease places a heavy economic burden to healthcare systems around the world. However, the effective treatments are still lacking. Traditional Chinese medicines (TCM) of Schisandra chinensis and Acorus tatarinowii Schott have the pharmacological effects of sedation and neuroprotection and have been clinically proven to be effective in the treatment of AD. However, their main anti-Alzheimer's compounds and functional mechanisms remain unclear. Purpose: To elucidate the main therapeutic components and possible mechanisms of Sc-At in AD using a comprehensive strategy combining metabolomics and network pharmacology. Methods: First, the UPLC-QTOF/MS method was used to identify the main chemical constituents of Schisandra chinensis and Acorus tatarinowii Schott alcohol extracts in vitro and in vivo. Secondly, the theoretical active ingredients, targets, and pathways of Sc-At for AD treatment were predicted by network pharmacology methods. Finally, plasma metabolomics were detected by UPLC-QTOF/MS to analyze the differential metabolites and metabolic pathways related to Sc-At. Based on the analyses above, the anti-AD mechanism of Sc-At was explored. Results: A total of 95 chemical components were identified in Sc-At extracts in vitro, and 34 prototype drug components were detected in rat plasma; network pharmacology screening identified 14 drug components in line with the principle of Lipinski, of which 10 were present for in vitro drug composition analysis. For these 10 components, 58 AD disease targets were predicted, and 85 AD-related KEGG signaling pathways were enriched. Six core biomarkers of Sc-At (cis-8,11,14,17-eicosatetraenoic acid, prostaglandin H2, sphingosine 1-phosphate, enol-phenylpyruvate, 3-Methoxytyrosine, and pristanoyl-CoA) were regulated to a normal state during the treatment of AD. Conclusion: The mechanism of Sc-At for the treatment of AD can be achieved by the effect of the 10 compounds of Sc-At on TNF, MAPK8, MAPK14, PTGS1, and other targets, thereby affecting arachidonic acid metabolism, neurotransmitters, and sphingolipid metabolism.

Demonstration of 3-Methoxytyrosine in the urine of melanoma patients

Acta Derm Venereol 1976;56(1):27-31.PMID:56853doi

With the aid of gas chromatography and mass spectrometry a metabolite of dopa, 3-Methoxytyrosine, has been demonstrated in the urine of 2 patients with melanoma metastases, the amounts excreted being 4.0 mg and 8.5 mg/24 hours, respectively. 3-Methoxytyrosine could not with certainty be identified in the urines of two normal subjects.