2-Thiobarbituric Acid
(Synonyms: 4,6-二羟基-2-巯基嘧啶, TBA, 硫代巴比妥酸) 目录号 : GC42195
A colorimetric reagent for the detection of lipid peroxidation
Cas No.:504-17-6
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
2-Thiobarbituric acid is a colorimetric reagent commonly used in the detection of malondialdehyde (MDA), a marker of lipid peroxidation. 2-Thiobarbituric acid forms a complex with MDA that can be quantified by colorimetric detection at 532 nm as a measure of lipid peroxidation.
| Cas No. | 504-17-6 | SDF | |
| 别名 | 4,6-二羟基-2-巯基嘧啶, TBA, 硫代巴比妥酸 | ||
| Canonical SMILES | O=C(CC(N1)=O)NC1=S | ||
| 分子式 | C4H4N2O2S | 分子量 | 144.1 |
| 溶解度 | DMF: 12 mg/ml,DMSO: 12 mg/ml,Ethanol: 0.2 mg/ml,PBS (pH 7.2): 0.5 mg/ml | 储存条件 | Store at -20°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 6.9396 mL | 34.6981 mL | 69.3963 mL |
| 5 mM | 1.3879 mL | 6.9396 mL | 13.8793 mL |
| 10 mM | 0.694 mL | 3.4698 mL | 6.9396 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Photodynamic activity and photoantimicrobial chemotherapy studies of ferrocene-substituted 2-Thiobarbituric Acid
Bioorg Med Chem Lett 2021 May 15;40:127922.PMID:33705910DOI:10.1016/j.bmcl.2021.127922.
A ferrocene-substituted thiobarbituric acid (FT) has been synthesized to explore its photophysical properties and photodynamic and photoantimicrobial chemotherapy activities. FT has an intense metal-to-ligand charge transfer (MLCT) band at ca. 575 nm. The ferrocene moiety of FT undergoes photooxidation to form a ferrocenium species which in turn produces hydroxyl radical in an aqueous environment, which was confirmed via the bleaching reaction of p-nitrosodimethylaniline (RNO). FT exhibits efficient PDT activity against MCF-7 cancer cells with an IC50 value of 5.6 μM upon irradiation with 595 nm for 30 min with a Thorlabs M595L3 LED (240 mW cm-2). Photodynamic inactivation of Staphylococcus aureus and Escherichia coli by FT shows significant activity with log reduction values of 6.62 and 6.16 respectively, under illumination for 60 min at 595 nm. These results demonstrate that ferrocene-substituted thiobarbituric acids merit further study for developing novel bioorganometallic PDT agents.
In situ formation of 2-Thiobarbituric Acid incorporated g-C3N4 for enhanced visible-light-driven photocatalytic performance
RSC Adv 2021 Jun 14;11(34):21084-21096.PMID:35479385DOI:10.1039/d1ra02121d.
Embedding heterocycles into the skeleton of g-C3N4 has been proved to be a simple and efficient strategy for improving light response and the separation of photo-excited charges. Herein, 2-Thiobarbituric Acid incorporated g-C3N4 (TBA/CN) with good photocatalytic efficiency for Rh B degradation and H2 production was successfully achieved via a facile thermal copolymerization approach. The incorporation of aromatics and S atoms into the skeleton of g-C3N4 was identified via systematic characterizations. This unique structure contributed to the narrowed band-gap, extended delocalization of lone pair electrons and changed electron transition pathway, which led to the enhanced visible light utilization, accelerated charge migration and prolonged electron lifetime, subsequently resulting in the significant boost of photocatalytic activity. The optimal TBA/CN-3 sample yielded the largest Rh B degradation rate constant k value of 0.0273 min-1 and simultaneously highest rate of H2 evolution of 0.438 mmol g-1 h-1, which were almost 3.5 and 3.8 folds as fast as that of the pristine CN, respectively. Finally, the photocatalytic mechanism was proposed for the detailed elucidation of the process of Rh B degradation coupled with H2 production.
Publisher Correction: Eco-friendly 2-Thiobarbituric Acid as a corrosion inhibitor for API 5L X60 steel in simulated sweet oilfield environment: Electrochemical and surface analysis studies
Sci Rep 2020 Feb 6;10(1):2383.PMID:32024925DOI:10.1038/s41598-020-59140-x.
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Synthesis, structural characterization and biological studies of the triphenyltin(IV) complex with 2-Thiobarbituric Acid
Eur J Med Chem 2011 Jul;46(7):2835-44.PMID:21521629DOI:10.1016/j.ejmech.2011.04.005.
The reaction between 2-Thiobarbituric Acid (H(2)TBA), which was treated with an equimolar amount of potassium hydroxide, in a water with triphenytin chloride in methanol, results in the formation of the {[Ph(3)Sn(O-HTBA)]}(n) (1) complex. Crystals of the hydrated 1 with formula {[Ph(3)Sn(O-HTBA)]·0.7(H(2)O)}(n) were growth from methanol/acetonitrile solution, of the white precipitation, filtered off, from the reaction. The crystal structure of complex 1 has been determined by X-ray diffraction at 120 K. Complex 1 is polymeric. The geometry around the tin(IV) ions is trigonal bi-pyramidal with coordination to three C atoms from phenyl groups and one O atom from a de-protonated HTBA ligand. Complex 1 and the already known [(n-Bu)(3)Sn(O-HTBA)·H(2)O] (2) were evaluated for their in vitro cytotoxic activity (cell viability) against human cancer cell lines: HeLa (cervical), OAW-42 (ovarian), MCF-7 (breast, ER positive), MDA-MB-231 (breast, ER negative), A549 (lung), Caki-1 (renal) and additionally, the normal human lung cell line MRC-5 (normal human fetal lung fibroblast cells) and normal immortalized human mammary gland epithelial cell line MTSV17 with a Trypan Blue assay. Moreover complex 1 was evaluated for its in vitro cell growth proliferation activity against leiomyosarcoma cells (LMS), MCF-7 and MRC-5 cells with a Thiazolyl Blue Tetrazolium Bromide (MTT) assay. The type of cell death caused by complexes 1 and 2 was also evaluated by use of flow cytometry assay. The results showed that these compounds mediate a strong cytotoxic response to normal and cancer cell lines tested through apoptosis and induce cell cycle arrest in S phase of the cell cycle, suggesting DNA intercalation (direct or indirect) with the complexes. Finally, the influence of these complexes 1 and 2 upon the catalytic peroxidation of linoleic acid to hydroperoxylinoleic acid by the enzyme lipoxygenase (LOX) was kinetically and theoretically studied.
Colorimetric speciation analysis of chromium using 2-Thiobarbituric Acid capped silver nanoparticles
Anal Methods 2020 May 21;12(19):2484-2490.PMID:32930238DOI:10.1039/d0ay00160k.
In the present work, 2-Thiobarbituric Acid (TBA)-capped silver nanoparticles (AgNPs) have been introduced as a novel nanoprobe in the construction of a sensitive and selective colorimetric sensor for Cr(iii) and Cr(vi) ions. Cr(iii) can be linked to TBA on the AgNP surface via -N or -OH groups and form a bridge between two or more AgNPs causing their aggregation. This phenomenon leads to a dramatic color change in TBA-AgNPs from yellow to pinkish red. UV-Vis spectrophotometry, FT-IR and transmission electron microscopy (TEM) were utilized for quantitative analyses, characterization of modified AgNPs and determination of the interaction mechanism of TBA-AgNPs - Cr(iii). The color change depended on the concentration of Cr(iii) and the ratio of UV-Vis absorption intensity at 520 nm to that at 418 nm (A520/A418) was linearly proportional to the concentration of Cr(iii) from 0.012 to 3.25 mg L-1 (LOD = 8.4 µg L-1). To eliminate or reduce the interference of other cations in Cr(iii) determination, EDTA was used as a masking agent at pH 6.5. Moreover, the developed method was used for the determination of Cr(vi) in the presence of ascorbic acid (AA) (as a reducing agent) in the concentration range of 0.20 to 1.40 mg L-1. The proposed probe was successfully applied for the determination of Cr(iii) and Cr(vi) in cement factory wastewater.