11-deoxy Corticosterone
(Synonyms: 去氧皮质酮) 目录号 : GC40394
11-deoxy Corticosterone是一种由肾上腺产生的类固醇激素，具有盐皮质激素活性，并作为醛固酮的前体。
Cas No.:64-85-7
Sample solution is provided at 25 µL, 10mM.
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11-deoxy Corticosterone is a steroid hormone produced by the adrenal gland that possesses mineralocorticoid activity and acts as an aldosterone precursor[1]. 11-deoxy Corticosterone is converted to aldosterone by 11β-hydroxylase, which adds a hydroxyl group at the 11th position[2]. Aldosterone is a hormone that regulates electrolyte balance and blood pressure by promoting sodium retention and potassium excretion[3]. 11-deoxy Corticosterone is usually used in the research of hypertension, inflammation, and metabolic disorders[4][5].
In vitro, 11-deoxy Corticosterone (10nM; 3h) significantly decreased glucose uptake in rainbow trout hepatocytes (RTH-149) and reduced apparent permeability in gill epithelial cells (RTgill-W1) after 3 hours of treatment[6].
In vivo, 11-deoxy Corticosterone (50mg/pellet; s.c.) every 21 days for 8 weeks increased cardiac fibrosis and inflammation in wild-type CBA/CaJ mice[7].
References:
[1] Silvestre JS, Robert V, Heymes C, et al. Myocardial production of aldosterone and corticosterone in the rat. Physiological regulation. J Biol Chem. 1998;273(9):4883-4891.
[2] Gao X, Yamazaki Y, Tezuka Y, et al. Pathology of Aldosterone Biosynthesis and its Action. Tohoku J Exp Med. 2021;254(1):1-15.
[3] Otsuka H, Abe M, Kobayashi H. The Effect of Aldosterone on Cardiorenal and Metabolic Systems. Int J Mol Sci. 2023;24(6):5370.
[4] Asla Q, Sardà H, Lerma E, et al. 11-Deoxycorticosterone Producing Adrenal Hyperplasia as a Very Unusual Cause of Endocrine Hypertension: Case Report and Systematic Review of the Literature. Front Endocrinol (Lausanne). 2022;13:846865.
[5] Sturm A, Bury N, Dengreville L, et al. 11-deoxycorticosterone is a potent agonist of the rainbow trout (Oncorhynchus mykiss) mineralocorticoid receptor. Endocrinology. 2005;146(1):47-55.
[6] Zuloaga R, Molina A, Valdés JA. In vitro effects of 11-deoxycorticosterone on hepatocytes and gill epithelial cells of rainbow trout (Oncorhynchus mykiss). Gen Comp Endocrinol. 2025;371:114776.
[7] Fletcher EK, Morgan J, Kennaway DR, et al. Deoxycorticosterone/Salt-Mediated Cardiac Inflammation and Fibrosis Are Dependent on Functional CLOCK Signaling in Male Mice. Endocrinology. 2017;158(9):2906-2917.
11-deoxy Corticosterone是一种由肾上腺产生的类固醇激素，具有盐皮质激素活性，并作为醛固酮的前体[1]。11-deoxy Corticosterone通过11β-羟化酶在第11位添加一个羟基转化为醛固酮[2]。醛固酮是一种通过促进钠潴留和钾排泄来调控电解质平衡和血压的激素[3]。11-deoxy Corticosterone通常用于研究高血压、炎症和代谢紊乱[4][5]。
在体外实验中，11-deoxy Corticosterone（10nM；3小时）显著降低了虹鳟鱼肝细胞（RTH-149）中的葡萄糖摄取，并减少了鳃上皮细胞（RTgill-W1）的表观通透性[6]。
在体内实验中，11-deoxy Corticosterone（50mg/pellet皮下注射；每21天一次）在8周内增加了野生型CBA/CaJ小鼠的心脏纤维化和炎症[7]。
| Cell experiment [1]: | |
Cell lines | rainbow trout hepatocytes (RTH-149) and gill epithelial cells (RTgill-W1) |
Preparation Method | The gill epithelium (RTgill-W1) cells were maintained at 19℃ without CO2 in the growth medium of Leibowitz L-15 with 2.05mM L-glutamine, supplemented with 10% of fetal bovine serum, and a 1% antibiotic/antimycotic solution. The hepatoma cells of the rainbow trout cell line (RTH-149) were grown at 20℃ without CO2 in minimum essential medium/Earles balanced salts (MEM/EBSS) containing 2mM L-glutamine supplemented with 10% of fetal bovine serum, 1% MEM nonessential amino acid (NEAA) solution, 10mM sodium pyruvate, 25mM HEPES and a 1% antibiotic/antimycotic solution. Both cell lines were harvested at 90% confluence using Trypsin EDTA solution, counted, and seeded to full confluence in 12-well plates. After 2 days, cells were washed twice with 1X PBS before treatments, according to experiments. For assays, the cells were treated with 11-deoxy Corticosterone (10nM) or vehicle solution (1X PBS-DMSO) for 3h together with the fluorescent D-glucose analog 2-NBDG (50µM), as a tracer to monitor glucose uptake in cells for fluorescence imaging microscopy. The apparent permeability values (Papp) were quantified in RTgill-W1 monolayers cultured on transwell inserts. |
Reaction Conditions | 10nM; 3h |
Applications | 11-deoxy Corticosterone (10nM; 3h） significantly decreased glucose uptake in rainbow trout hepatocytes (RTH-149) and reduced apparent permeability in gill epithelial cells (RTgill-W1). |
| Animal experiment [2]: | |
Animal models | CBA/CaJ mice |
Preparation Method | CBA/CaJ mice were maintained under 12h light dark cycles (lights on 8AM, lights off 8PM), uninephrectomized and given normal chow plus 1% saline to drink ad lib. For experiment, 8-wk-old male CBA/CaJ mice were randomly assigned to receive no further treatment (‘vehicle’, VEH) or a 21 day slow release 11-deoxy Corticosterone pellet (50mg, s.c) that was replaced after 21 and 42 days. Systolic blood pressure (SBP) was measured at 4 and 8wk in pre-trained mice by tail-cuff plethysmography At 8wk, arterial blood was collected by cardiac puncture, and the heart and kidney dissected and fixed (10% formalin in 0.1M phosphate buffer solution (PBS; pH 7.4) or rapidly snap frozen in liquid nitrogen. All mice were culled between 8AM and 9AM zietgeber time (ZG) 0-1. Net cardiac tissue fibrosis was determined by Sirius red staining. Cardiac inflammation was assessed by immunohistochemistry for related markers. |
Dosage form | 50mg/pellet; s.c. every 21 days for 8 weeks |
Applications | 11-deoxy Corticosterone (50mg/pellet; s.c.) every 21 days for 8 weeks increased cardiac fibrosis and inflammation in wild-type CBA/CaJ mice. |
References: | |
| Cas No. | 64-85-7 | SDF | |
| 别名 | 去氧皮质酮 | ||
| Canonical SMILES | O=C1CC[C@@]2(C)C(CC[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@@H]4C(CO)=O)=C1 | ||
| 分子式 | C21H30O3 | 分子量 | 330.5 |
| 溶解度 | DMF: 25 mg/ml,DMSO: 25 mg/ml,Ethanol: 25 mg/ml,Ethanol:PBS(pH 7.2) (1:1): 0.5 mg/ml | 储存条件 | Store at -20°C |
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.0257 mL | 15.1286 mL | 30.2572 mL |
| 5 mM | 605.1 μL | 3.0257 mL | 6.0514 mL |
| 10 mM | 302.6 μL | 1.5129 mL | 3.0257 mL |
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动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
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| % DMSO % % Tween 80 % saline | ||||||||||
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2.
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