11-deoxy Corticosterone
(Synonyms: 去氧皮质酮) 目录号 : GC40394An endogenous mineralocorticoid
Cas No.:64-85-7
Sample solution is provided at 25 µL, 10mM.
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11-deoxy Corticosterone (DOC) is an endogenous mineralocorticoid synthesized in the zona fasciculata and zona glomerulosa of the adrenal gland. It is a metabolite of progesterone and precursor to aldosterone and corticosterone . DOC is metabolized to the neuroactive compound (3α,5α)-2,21-dihydroxypregnan-20-one (THDOC), which positively modulates GABAA receptors and produces effects similar to barbiturates in rats. Injections of naloxone and corticotropin-releasing hormone (CRH) increase, while dexamethasone decreases, DOC in cynomolgus monkeys. DOC levels are increased 3.4-fold in obese and diabetic mice.
Cas No. | 64-85-7 | SDF | |
别名 | 去氧皮质酮 | ||
Canonical SMILES | O=C1CC[C@@]2(C)C(CC[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@@H]4C(CO)=O)=C1 | ||
分子式 | C21H30O3 | 分子量 | 330.5 |
溶解度 | DMF: 25 mg/ml,DMSO: 25 mg/ml,Ethanol: 25 mg/ml,Ethanol:PBS(pH 7.2) (1:1): 0.5 mg/ml | 储存条件 | Store at -20°C |
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1 mg | 5 mg | 10 mg | |
1 mM | 3.0257 mL | 15.1286 mL | 30.2572 mL |
5 mM | 0.6051 mL | 3.0257 mL | 6.0514 mL |
10 mM | 0.3026 mL | 1.5129 mL | 3.0257 mL |
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2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
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Taenia crassiceps WFU cysticerci synthesize corticosteroids in vitro: metyrapone regulates the production
Gen Comp Endocrinol 2012 May 1;176(3):409-14.PMID:22321721DOI:10.1016/j.ygcen.2012.01.015.
Taenia solium and Taenia crassiceps WFU cysticerci and tapeworms have the ability to synthesize sex steroid hormones and have a functional 3β-hydroxisteroid dehydrogenase. Corticosteroids (CS) like corticosterone and dexamethasone have been shown to stimulate in vitro estrogen production by Taenia crassiceps WFU cysticerci. The aim of this work was to study the ability of T. crassiceps WFU cysticerci to synthesize corticosteroids, and the effect of the inhibitor metyrapone on the CS synthesis. For this purpose T. crassiceps WFU cysticerci were obtained from the abdominal cavity of mice, thoroughly washed and pre-incubated in multiwells for 24 h in DMEM plus antibiotics/antimycotics. The tritiated CS precursor progesterone ((3)H-P4) was added to the culture media and parasites cultured for different periods. Blanks containing the culture media plus the (3)H-P4 were simultaneously incubated. Blanks and parasite culture media were ether extracted and analyzed by thin layer chromatography (TLC) in two different solvent systems. Corticosterone production was measured in the culture media by RIA. In some experiments metyrapone (0.1-0.5 mM) was added for 24, 48 or 72 h. Results showed that cysticerci mainly synthesized tritiated 11-deoxy Corticosterone (DOC) and small amounts of corticosterone that was also detected by RIA. Small amounts of (3)H-11-deoxy cortisol were also found. Corticosteroid synthesis was time dependent. The addition of metyrapone significantly inhibited tritiated DOC, deoxycortisol and corticosterone synthesis. These results show for the first time that parasites have the capacity to synthesize CS that is modulated by metyrapone. Data suggest that DOC is the main corticosteroid in the parasites.