Patulin
(Synonyms: 棒曲霉素; Terinin) 目录号 : GC40483Patulin是一种有毒的多酚类乳内酯。
Cas No.:149-29-1
Sample solution is provided at 25 µL, 10mM.
Patulin is a toxic polyphenolic lactolactone [1]. Patulin reacts with protein sulfhydryl groups, triggering cellular oxidative stress and p53-mediated DNA damage, which damages the intestinal epithelial barrier [2]. Patulin has teratogenic, genotoxic, and neurotoxic properties [3-4].
In HL-60 cells, after Patulin (0-10μM; 6h) significantly decreased cell viability [5]. In HepG2 cells, Patulin (0.5-2μM; 24h) induces autophagy in cells via the ROS-Akt1-MTOR pathway [6].
In CF-1 mice, Patulin (1.0-3.75mg/kg; ip; single injection) induced DNA strand breaks, decreased GSH levels, and increased lipid peroxidation in the brain, liver, and kidney [7]. In B16F10 cell xenograft mouse model, Patulin (5-50μg/kg; ip; 20d) significantly induced tumor regression [8].
References:
[1]. Puel O, Galtier P, Oswald I P. Biosynthesis and toxicological effects of patulin[J]. Toxins, 2010, 2(4): 613-631.
[2]. Saxena N, Ansari K M, Kumar R, et al. Patulin causes DNA damage leading to cell cycle arrest and apoptosis through modulation of Bax, p53 and p21/WAF1 proteins in skin of mice[J]. Toxicology and applied pharmacology, 2009, 234(2): 192-201.
[3]. Glaser N, Stopper H. Patulin: Mechanism of genotoxicity[J]. Food and Chemical Toxicology, 2012, 50(5): 1796-1801.
[4]. Lei W L, Li Y Y, Hou Y, et al. Toxic effects of patulin on mouse oocytes and its possible mechanisms[J]. Toxicology, 2021, 464: 153013.
[5]. Wu T S, Liao Y C, Yu F Y, et al. Mechanism of patulin-induced apoptosis in human leukemia cells (HL-60)[J]. Toxicology letters, 2008, 183(1-3): 105-111.
[6]. Yang G, Bai Y, Wu X, et al. Patulin induced ROS-dependent autophagic cell death in Human Hepatoma G2 cells[J]. Chemico-Biological Interactions, 2018, 288: 24-31.
[7]. de Melo F T, de Oliveira I M, Greggio S, et al. DNA damage in organs of mice treated acutely with patulin, a known mycotoxin[J]. Food and Chemical Toxicology, 2012, 50(10): 3548-3555.
[8]. Boussabbeh M, Ben Salem I, Rjiba-Touati K, et al. The potential effect of patulin on mice bearing melanoma cells: an anti-tumour or carcinogenic effect?[J]. Tumor Biology, 2016, 37(5): 6285-6295.
Patulin是一种有毒的多酚类乳内酯 [1]。Patulin可与蛋白质巯基发生反应，引发细胞氧化应激和p53介导的DNA损伤，从而破坏肠道上皮屏障 [2]。Patulin具有致畸、遗传毒性和神经毒性 [3-4]。
在HL-60细胞中，Patulin（0-10μM；6h）治疗后细胞活力显著下降 [5]。在HepG2细胞中，Patulin（0.5-2μM；24h）通过ROS-Akt1-MTOR通路诱导细胞自噬 [6]。
在CF-1小鼠中，Patulin（1.0-3.75mg/kg；ip；单次注射）诱导DNA链断裂，降低谷胱甘肽（GSH）水平，并增加脑、肝和肾中的脂质过氧化 [7]。在B16F10细胞异种移植小鼠模型中，Patulin（5-50μg/kg；ip；20d）显著诱导肿瘤消退 [8]。
| Cell experiment [1]: | |
Cell lines | HL-60 cells |
Preparation Method | HL-60 (1 × 105 cells) were seeded in 96-well plates, treated with vehicle (15% ethanol in 0.01M PBS) alone or various concentrations (final concentration 0-10μM) of Patulin at the designated time. |
Reaction Conditions | 0-10μM; 6h |
Applications | Patulin treatment significantly decreased cell viability. |
| Animal experiment [2]: | |
Animal models | CF-1 mice |
Preparation Method | The experimental design involved nine groups of 10 mice, housed individually in cages. Mice received a single intraperitoneal (i.p.) dose of 1.0, 2.5, or 3.75mg/kg body weight (BW) of Patulin. Patulin stock solution was dissolved in DMSO and then diluted with saline to a final DMSO concentration below 0.1%. Mice were divided into the following groups: Group 1: Control group, administered with 0.1% DMSO in saline (5mL/kg BW). Group 2: Positive control group, administered with MMS (40mg/kg BW). Groups 3-5: Patulin was administered intraperitoneally at the following doses: Group 3, 1.0mg/kg BW; Group 4, 2.5mg/kg BW; Group 5, 3.75mg/kg BW. Group 6: N-ac (1840μmol/kg BW) was administered 3 hours prior to administration of 0.1% DMSO (5mL/kg BW). Groups 7-9 were intraperitoneally injected with Patulin at the following doses: 1.0mg/kg body weight for Group 7; 2.5mg/kg body weight for Group 8; and 3.75mg/kg body weight for Group 9. All animals in Groups 7-9 were given 300mg/kg of N-ac 3 hours before Patulin. |
Dosage form | 1.0-3.75mg/kg; ip; single injection |
Applications | Patulin induced DNA strand breaks, decreased GSH levels, and increased lipid peroxidation in the brain, liver, and kidney. |
References: | |
| Cas No. | 149-29-1 | SDF | |
| 别名 | 棒曲霉素; Terinin | ||
| 化学名 | 4-hydroxy-4H-furo[3,2-c]pyran-2(6H)-one | ||
| Canonical SMILES | O=C1OC2=CCOC(O)C2=C1 | ||
| 分子式 | C7H6O4 | 分子量 | 154.1 |
| 溶解度 | 13mg/mL in ethanol, 10mg/mL in DMSO, 20mg/mL in DMF | 储存条件 | -20°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 6.4893 mL | 32.4465 mL | 64.8929 mL |
| 5 mM | 1.2979 mL | 6.4893 mL | 12.9786 mL |
| 10 mM | 0.6489 mL | 3.2446 mL | 6.4893 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
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