Oleic Acid methyl ester
(Synonyms: 油酸甲酯) 目录号 : GC40346
An esterified version of oleic acid
Cas No.:112-62-9
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Oleic acid methyl ester is an esterified version of the free acid which is less water soluble but more amenable for the formulation of oleate-containing diets and dietary supplements. Oleic acid is a monounsaturated fatty acid and is one of the major components of membrane phospholipids. Oleic acid contributes about 17% of the total fatty acids esterified to phosphatidylcholine, the major phospholipid class in porcine platelets.
| Cas No. | 112-62-9 | SDF | |
| 别名 | 油酸甲酯 | ||
| Canonical SMILES | CCCCCCCC/C=C\CCCCCCCC(OC)=O | ||
| 分子式 | C19H36O2 | 分子量 | 296.5 |
| 溶解度 | 0.15 M Tris-HCl pH 8.5: >1 mg/ml,DMF: >100 mg/ml,DMSO: >100 mg/ml,Ethanol: >100 mg/ml,PBS (pH 7.2): <100 µ g/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.3727 mL | 16.8634 mL | 33.7268 mL |
| 5 mM | 0.6745 mL | 3.3727 mL | 6.7454 mL |
| 10 mM | 0.3373 mL | 1.6863 mL | 3.3727 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Lipid deposition on silicone hydrogel lenses, part I: quantification of oleic Acid, Oleic Acid methyl ester, and cholesterol
Eye Contact Lens 2006 Dec;32(6):300-7.PMID:17099392DOI:10.1097/01.icl.0000224365.51872.6c.
Purpose: To investigate the sorption of oleic acid, Oleic Acid methyl ester, and cholesterol on currently marketed silicone hydrogel contact lenses. Methods: Two liquid chromatography methods were developed and used to analyze lens extracts from continuous-wear and daily-wear modalities from asymptomatic silicone hydrogel contact lens wearers. Results: Of the three probed compounds, cholesterol is the most prevalently sorbed, at levels ranging from below the limit of quantitation (<1.50 microg per lens) to approximately 37.0 microg per lens. Oleic acid and Oleic Acid methyl ester were found to exist at levels below the limit of quantitation (<1.50 microg per lens). In general, there appears to be no significant difference between the amounts of cholesterol sorbed on the continuous-wear PureVision and daily-wear PureVision lenses evaluated. Conclusions: The quantities of lipid sorbed to continuous-wear PureVision lenses are significantly different from those previously reported by other authors in a similarly conducted experiment. This difference suggests that any hypothesis of silicone hydrogel lenses based on these previous lipid data should be reconsidered.
Gas chromatography-vacuum ultraviolet spectroscopy for analysis of fatty acid methyl esters
Food Chem 2016 Mar 1;194:265-71.PMID:26471553DOI:10.1016/j.foodchem.2015.08.004.
A new vacuum ultraviolet (VUV) detector for gas chromatography was recently developed and applied to fatty acid methyl ester (FAME) analysis. VUV detection features full spectral acquisition in a wavelength range of 115-240nm, where virtually all chemical species absorb. VUV absorption spectra of 37 FAMEs, including saturated, monounsaturated, and polyunsaturated types were recorded. Unsaturated FAMEs show significantly different gas phase absorption profiles than saturated ones, and these classes can be easily distinguished with the VUV detector. Another advantage includes differentiating cis/trans-isomeric FAMEs (e.g. Oleic Acid methyl ester and linoleic acid methyl ester isomers) and the ability to use VUV data analysis software for deconvolution of co-eluting signals. As a universal detector, VUV also provides high specificity, sensitivity, and a fast data acquisition rate, making it a powerful tool for fatty acid screening when combined with gas chromatography. The fatty acid profile of several food oil samples (olive, canola, vegetable, corn, sunflower and peanut oils) were analyzed in this study to demonstrate applicability to real world samples.
Oleic acid causes apoptosis and dephosphorylates Bad
Neurochem Int 2005 Jan;46(2):127-35.PMID:15627513DOI:10.1016/j.neuint.2004.08.003.
There is increasing evidence showing the involvement of unsaturated free fatty acids in cell death pathways, particularly in the context of apoptotic signalling. Our previous in vitro study has demonstrated that oleic acid, a monounsaturated fatty acid, reduces phosphorylation of proapoptotic Bad through activation of protein phosphatase type 2Cbeta. In the present study, we attempted to investigate the role of oleic acid in neuronal apoptosis using different types of cell cultures, and, furthermore, to explore the underlying mechanism with regard to its effect on Bad expression. As revealed by nuclear staining, oleic acid caused a concentration- and time-dependent damage with typical apoptotic features in cortical and hippocampal cultures from embryonic and neonatal rats, respectively, as well as in human neuroblastoma SH-SY5Y cells. In mixed hippocampal cultures, nearly all neurons were damaged at 24 h after the treatment, while damage of astrocytes was detected 48 h after adding this fatty acid, suggesting that neurons were more vulnerable than astrocytes. Nile blue staining showed that oleic acid and Oleic Acid methyl ester were both taken up by the neurons within 30 min. In contrast to oleic acid, Oleic Acid methyl ester did not change cell viability demonstrating that oleic acid-induced cell death was not due to an overload of the cells with lipids. Caspase-3 activity was not increased by oleic acid in cultured hippocampal cells. Western blot analysis of phospho-Ser112 Bad and the total Bad in cultured hippocampal cells revealed a significant decrease in the ratio of phospho-Ser112 Bad to total Bad in a time- and concentration-dependent manner after the exposure with oleic acid. We conclude that oleic acid induces neuronal apoptosis through a caspase-3-independent mechanism involving dephosphorylation of Bad.
Pilot scale wastewater treatment, CO2 sequestration and lipid production using microalga, Neochloris aquatica RDS02
Int J Phytoremediation 2020;22(14):1462-1479.PMID:32615792DOI:10.1080/15226514.2020.1782828.
In present investigation carried out large-scale treatment of tannery effluent by the cultivation of microalgae, Neochloris aquatica RDS02. The tannery effluent treatment revealed that significant reduction heavy metals were chromium-3.59, lead-2.85, nickel-1.9, cadmium-10.68, zinc-4.49, copper-0.95 and cobalt-1.86 mg/L on 15th day of treatment using N. aquatica RDS02. The microalgal biosorption capacity q max rate was Cr-88.66, Pb-75.87, Ni-87.61, Cd-60.44, Co-52.86, Zn-84.90 and Cu-54.39, and isotherm model emphasized that the higher R 2 value 0.99 by Langmuir and Freundlich kinetics model. The microalga utilized highest CO2 (90%) analyzed by CO2 biofixation and utilization kinetics, biomass (3.9 mg/mL), lipid (210 mg mL-1), carbohydrate (102.75 mg mL-1), biodiesel (4.9 mL g-1) and bioethanol (4.1 mL g-1). The microalgal-lipid content was analyzed through Nile red staining. Gas chromatography mass spectrometric (GCMS) analysis confirmed that the presence of a biodiesel and major fatty acid methyl ester (FAME) profiling viz., tridecanoic acid methyl ester, pentadecanoic acid methyl ester, octadecanoic acid methyl ester, myristic acid methyl ester, palmitic acid methyl ester and Oleic Acid methyl ester. Fourier transform infrared (FTIR) analysis confirmed that the presence of a functional groups viz., phenols, alcohols, alkynes, carboxylic acids, ketones, carbonyl and ester groups. The bioethanol production was confirmed by high-performance liquid chromatography (HPLC) analyze.