Gly-Pro-pNA (hydrochloride)
(Synonyms: N-甘氨酰脯氨酰-对硝基苯胺盐酸盐) 目录号 : GC18201
Gly-Pro-pNA（盐酸盐）是一种显色底物，可被循环酶二肽基肽酶 IV (DPP IV) 裂解。
Cas No.:103213-34-9
Sample solution is provided at 25 µL, 10mM.
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Gly-Pro-pNA (hydrochloride) is a chromogenic substrate that can be cleaved by the circulating enzyme, dipeptidyl peptidase IV (DPP IV). Enzyme activity can be quantified by colorimetric detection of free p-nitroanilide at 405 nm. This substrate can be used to screen for DPP IV inhibitors.[5].
Mare whey proteins show good DPP-IV inhibitory activity in vitro，have the able to inhibit DPP-IV from degrading Gly-Pro-pNA (hydrochloride) Gly-Pro-pNA (hydrochloride) [1]. The in vitro DPP-IV inhibitory activities of quinoa protein hydrolysates can determined using Gly-Pro-pNA (hydrochloride) as a substrate[2]. Gly-Pro-pNA (hydrochloride) can also be used to evaluate the activity of DPP-IV in human serum[3]. Demonstrating that DPP IV in diabetic patients is not very different from that in normal conditions[4]. Gly-pro p-nitroanilide hydrochloride was used as a substrate to detect DPPIV level in patients with Polycystic syndrome (PCOS) ，a deregulation of DPP4 serum levels could be an additional characteristic of the metabolic imbalances associated with PCOS[5]
Using Gly-Pro-pNA (hydrochloride) as substrate can assess the enzymatic activity and biochemical status of dipeptidyl peptidase IV (DPP IV)in patients with rheumatoid arthritis[6]
References:
[1]. Song JJ, Wang Q, et,al. Identification of dipeptidyl peptidase-IV inhibitory peptides from mare whey protein hydrolysates. J Dairy Sci. 2017 Sep;100(9):6885-6894. doi: 10.3168/jds.2016-11828. Epub 2017 Jul 12. PMID: 28711271.
[2]. You H, Wu T, et,al. Preparation and identification of dipeptidyl peptidase IV inhibitory peptides from quinoa protein. Food Res Int. 2022 Jun;156:111176. doi: 10.1016/j.foodres.2022.111176. Epub 2022 Mar 19. PMID: 35651037.
[3]. Yeganeh F, Mousavi SMJ, et,al. Association of CD26/dipeptidyl peptidase IV mRNA level in peripheral blood mononuclear cells with disease activity and bone erosion in rheumatoid arthritis. Clin Rheumatol. 2018 Dec;37(12):3183-3190. doi: 10.1007/s10067-018-4268-y. Epub 2018 Aug 22. PMID: 30136129.
[4]. Sun AL, Deng JT, et,al. Dipeptidyl peptidase-IV is a potential molecular biomarker in diabetic kidney disease. Diab Vasc Dis Res. 2012 Oct;9(4):301-8. doi: 10.1177/1479164111434318. Epub 2012 Mar 2. PMID: 22388283.
[5]. Blauschmidt S, Greither T, et,al. Dipeptidyl peptidase 4 serum activity and concentration are increased in women with polycystic ovary syndrome. Clin Endocrinol (Oxf). 2017 Dec;87(6):741-747. doi: 10.1111/cen.13444. Epub 2017 Sep 13. PMID: 28799235.
[6]. Mavropoulos JC, Cuchacovich M, et,al. Anti-tumor necrosis factor-alpha therapy augments dipeptidyl peptidase IV activity and decreases autoantibodies to GRP78/BIP and phosphoglucose isomerase in patients with rheumatoid arthritis. J Rheumatol. 2005 Nov;32(11):2116-24. PMID: 16265688.
Gly-Pro-pNA（盐酸盐）是一种显色底物,可被循环酶二肽基肽酶 IV (DPP IV) 裂解。酶活性可以通过在 405 nm 处游离对硝基苯胺的比色检测来量化。该底物可用于筛选 DPP IV 抑制剂。[5]。
马乳清蛋白在体外表现出良好的DPP-IV抑制活性,能够抑制DPP-IV降解Gly-Pro-pNA (hydrochloride) Gly-Pro-pNA (hydrochloride) [1]。以 Gly-Pro-pNA（盐酸盐）为底物,可以测定藜麦蛋白水解物的体外 DPP-IV 抑制活性[2]。 Gly-Pro-pNA（盐酸盐）也可用于评价 DPP-IV 在人血清中的活性[3]。证明糖尿病患者的 DPP IV 与正常情况下的差异不大[4]。以甘氨酰对硝基苯胺盐酸盐为底物检测多囊综合征（PCOS）患者的DPPIV水平,DPP4血清水平失调可能是PCOS相关代谢失衡的另一个特征[5]
以Gly-Pro-pNA（盐酸盐）为底物可评估类风湿性关节炎患者二肽基肽酶IV（DPP IV）的酶活性和生化状态[6]
| Cell experiment [1]: | |
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Cell lines |
Mare whey protein hydrolysates |
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Preparation Method |
20 uL of sample solution and 100 uL of substrate solution (H- Gly-Pro-pNA (hydrochloride), final concentration 0.5 mM) were added. Enzyme reaction was started by adding DPP-IV. The absorbance at 405 nm was monitored after the microplate was incubated at 37°C for 0.5 h. |
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Reaction Conditions |
Final concentration 0.5 mM ，37 °C for 0.5 h. |
|
Applications |
The degradation rate of the substrate Gly-Pro-pNA (hydrochloride) of DPP-IV was slowed by the addition of Mare Whey proteins. |
|
References: [1]. Song JJ, Wang Q, et,al. Identification of dipeptidyl peptidase-IV inhibitory peptides from mare whey protein hydrolysates. J Dairy Sci. 2017 Sep;100(9):6885-6894. doi: 10.3168/jds.2016-11828. Epub 2017 Jul 12. PMID: 28711271. |
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| Cas No. | 103213-34-9 | SDF | |
| 别名 | N-甘氨酰脯氨酰-对硝基苯胺盐酸盐 | ||
| 化学名 | glycyl-N-(4-nitrophenyl)-L-prolinamide, monohydrochloride | ||
| Canonical SMILES | O=C(CN)N1CCC[C@H]1C(NC2=CC=C([N+]([O-])=O)C=C2)=O.Cl | ||
| 分子式 | C13H16N4O4.HCl | 分子量 | 328.8 |
| 溶解度 | 30mg/mL in DMSO, 30mg/mL in DMF, 30mg/mL in Ethanol | 储存条件 | Store at -20°C,stored under nitrogen |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.0414 mL | 15.2068 mL | 30.4136 mL |
| 5 mM | 0.6083 mL | 3.0414 mL | 6.0827 mL |
| 10 mM | 0.3041 mL | 1.5207 mL | 3.0414 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet