|BMPO 目录号 GC41397|
Sample solution is provided at 25 µL, 10mM.
|Cas No.||387334-31-8||SDF||Download SDF|
|化学名||3,4-dihydro-2-methyl-1,1-dimethylethyl ester-2H-pyrrole-2-carboxylic acid-1-oxide|
|溶解度||33mg/mL in ethanol, 25mg/mL in DMSO, or in DMF||储存条件||Store at -20°C|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
|Shipping Condition||Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
BMPO is a cyclic nitrone spin trap that can be used for the detection and characterization of thiyl radicals, hydroxyl radicals, and superoxide anions in vitro or in vivo. The BMPO-superoxide adduct does not rapidly decompose to the hydroxyl adduct in cells (t½ = 23 min). Also, the ESR spectrum of the BMPO-glutathionyl adduct does not fully overlap with the spectrum of its hydroxyl adduct. Purified by crystallization and stored as a solid, BMPO has a longer shelf life than liquid spin traps.
. Zhao, H., Joseph, J., Zhang, H., et al. Synthesis and biochemical applications of a solid cyclic nitrone spin trap: A relatively superior trap for detecting superoxide anions and glutathiyl radicals. Free Radic. Biol. Med. 31(5), 599-606 (2001).
. Khan, N., Wilmont, C.M., Rosen, G.M., et al. Spin traps: In vitro toxicity and stability of radical adducts. Free Radical Biology & Medicine 34(11), 1473-1481 (2003).