Alternariol
(Synonyms: 交链孢酚) 目录号 : GC10779
A mycotoxin
Cas No.:641-38-3
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Alternariol (AOH) is a toxic metabolite produced by Alternaria alternata and A. tenuissima, which grow on corn, rice, fruits, vegetables and oilseed. Mycotoxins are biologically active secondary fungal metabolites found as contaminants of food- and feedstuff [1].
Alternariol (0.8 μM) inhibited P4 secretion. Alternariol (1.6 μM) significantly reduced cell viability [1]. Alternariol (0.8 μM) reduced the expression of P450scc protein. Alternariol (1.6 μM) significantly reduced the abundance of α-tubulin and also clearly affected actin protein concentrations. Alternariol specifically inhibited P4 secretion in cultured porcine granulosa cells [1]. Twelve hours of light exposure was sufficient to decrease significantly the production of alternariol [2]. Light inhibited the production of alternariol in Alternaria alternate [2]. Alternariol was preferentially metabolized by cytochrome P450 (CYP) 1A1 and 1A2. Alternariol enhanced the levels of CYP1A1 in murine hepatoma cells (Hepa-1c1c7) but not in cells with inactivated AhR (Hepa-1c1c12) or ARNT (Hepa-1c1c4). Alternariol showed no effects on the production of reactive oxygen species but reduced cell counts in Hepa-1c1c7 cells after 24 and 48 h. At 48 h, AOH increased apoptosis dependent on AhR and ARNT [3].
References:
[1] Tiemann U, Tomek W, Schneider F, et al. The mycotoxins alternariol and alternariol methyl ether negatively affect progesterone synthesis in porcine granulosa cells in vitro[J]. Toxicology Letters, 2009, 186(2): 139-145.
[2] Sderhll K, Svensson E, Unestam T. Light inhibits the production of alternariol and alternariol monomethyl ether in Alternaria alternata[J]. Applied and environmental microbiology, 1978, 36(5): 655-657.
[3] Schreck I, Deigendesch U, Burkhardt B, et al. The Alternaria mycotoxins alternariol and alternariol methyl ether induce cytochrome P450 1A1 and apoptosis in murine hepatoma cells dependent on the aryl hydrocarbon receptor[J]. Archives of toxicology, 2012, 86(4): 625-632.
| Cas No. | 641-38-3 | SDF | |
| 别名 | 交链孢酚 | ||
| 化学名 | 3,7,9-trihydroxy-1-methyl-6H-dibenzo[b,d]pyran-6-one | ||
| Canonical SMILES | CC1=C(C2=C(C(O3)=O)C(O)=CC(O)=C2)C3=CC(O)=C1 | ||
| 分子式 | C14H10O5 | 分子量 | 258.2 |
| 溶解度 | ≤0.5mg/ml in ethanol;30mg/ml in DMSO;30mg/ml in dimethyl formamide | 储存条件 | Store at -20°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.873 mL | 19.3648 mL | 38.7297 mL |
| 5 mM | 0.7746 mL | 3.873 mL | 7.7459 mL |
| 10 mM | 0.3873 mL | 1.9365 mL | 3.873 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。