USP5 protein, Human (sf9, His)
目录号 : GC27238USP5 蛋白选择性裂解支链多泛素聚合物，有利于“Lys-48”连接的多泛素分解。它对线性和“Lys-63”连接的多聚泛蛋白表现出较低的亲和力。USP5 敲低导致 p53/TP53 积累，增强 p53/TP53 转录活性，因为与 MDM2 不同，未锚定的多聚泛素与泛素化的 p53/TP53 竞争蛋白酶体识别。USP5 蛋白, Human (sf9, His) 是重组的 USP5 蛋白，由 Sf9 insect cells 表达，带有 C-His 标签。
Sample solution is provided at 25 µL, 10mM.
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USP5 protein selectively cleaves branched multiubiquitin polymers, favoring 'Lys-48'-linked polyubiquitin disassembly. It exhibits lower affinity for linear and 'Lys-63'-linked polyubiquitin. USP5 knockdown leads to p53/TP53 accumulation, enhancing p53/TP53 transcriptional activity, as unanchored polyubiquitin competes with ubiquitinated p53/TP53 for proteasomal recognition, unlike MDM2. USP5 Protein, Human (sf9, His) is the recombinant human-derived USP5 protein, expressed by Sf9 insect cells , with C-His labeled tag.
USP5 是一种去泛素化酶，表现出对切割线性和支链多泛素聚合物的明显偏好，对支化结构具有特殊的亲和力。它积极参与未锚定“Lys-48”连接的多聚泛素链的分解。虽然 USP5 以相对较低的亲和力结合线性和“Lys-63”连接的多聚泛素，但其敲低会导致 p53/TP53 的积累和 p53/TP53 转录活性的升高。这种效应归因于在没有 USP5 的情况下，未锚定的多聚泛素能够与泛素化的 p53/TP53 竞争，但不与 MDM2 竞争蛋白酶体的识别。
| Purity | Greater than 90% as determined by reducing SDS-PAGE. | Source | Sf9 insect cells |
| Phycical Appearance | Lyophilized powder. | Shipping Condition | Room temperature in continental US; may vary elsewhere. |
| Synonyms | Ubiquitin carboxyl-terminal hydrolase 5; Isopeptidase T; USP5; ISOT | ||
| Stability | Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. | ||
| Endotoxin Level | <1 EU/μg, determined by LAL method. | ||
| Reconstitution | It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. | ||
| Formulation | Lyophilized from a 0.22 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80. | ||
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.