TRITC-dextran, MW 70000

TRITC-dextran, MW 70000 is a fluorescently labeled dextran used for tracing and studying cellular processes such as endocytosis, exocytosis, and cell labeling. The maximum absorbed/emitted wavelength of TRITC-dextran is 550/571nm, which has high stability and resistance to photobleaching^[1]. TRITC-dextran is widely used in biological research to label cells or intracellular regions and monitor various biological activities^[2]. TRITC-dextran can also be used to label neurons and neural networks to further investigate the mechanisms of neurotransmitter release and transmission^[3]. In addition, TRITC-dextran can serve as a packaging material to modify the surface properties of nanoparticles^[4].

In vitro, TRITC-dextran (10μg/ml) is added to the upper chamber of Transwells seeded with HT29 cell monolayers in the lower chamber. After incubation at 37°C for 1–4 hours, the fluorescence intensity of TRITC-dextran in the lower chamber is measured to reflect the permeability of the tight junctions in the cell monolayer^[5]. TRITC-dextran (2mg/ml) is added to the upper chamber of Transwells seeded with brain microvascular endothelial cells (BMECs) in the lower chamber. The fluorescence intensity of TRITC-dextran in the BMECs is detected to evaluate the integrity of the blood-brain barrier (BBB)^[6].

In vivo, TRITC-dextran can be used to assess the hepatic microcirculation in rats. TRITC-dextran (500mg/kg) is administered to rats via intravenous injection. One minute after administration, the liver is excised to obtain tissue samples. The liver tissue is fixed in 20% formaldehyde solution, embedded in paraffin, and sectioned. After deparaffinization, hydration, and washing with phosphate-buffered saline (PBS), the sections are immediately mounted with a Fluor Mount/plus mounting medium containing water to observe the distribution of TRITC-dextran under a fluorescence microscope^[7]. TRITC-dextran can also be used to evaluate the vascular perfusion in regenerated tissue after spinal cord injury. Mice are intravenously injected with 250 μL of a saline solution containing 10mg/mL TRITC-dextran. The amount of TRITC-dextran was quantitatively assessed by measuring the surface area of fluorescence in the field of view of the longitudinal image to detect vascular and systemic circulation^[8].

References:
[1] Kirby J, Standfest M, Binkley J, et al. The dynamin inhibitor, dynasore, prevents zoledronate-induced viability loss in human gingival fibroblasts by partially blocking zoledronate uptake and inhibiting endosomal acidification. J Appl Oral Sci. 2024 Sep 30;32:e20240224.
[2] Emura N, Sakurai N, Takahashi K, et al. OCT-4 expression is essential for the segregation of trophectoderm lineages in porcine preimplantation embryos. J Reprod Dev. 2016 Aug 25;62(4):401-8.
[3] Chang P, Xu M, Zhu J, et al, Pharmacological Inhibition of Mitochondrial Division Attenuates Simulated High-Altitude Exposure-Induced Memory Impairment in Mice: Involvement of Inhibition of Microglia-Mediated Synapse Elimination. CNS Neurosci Ther. 2025 Jun;31(6):e70473.
[4] Baek H, Yang SW, Kim MK, et al. Activation of Immune Responses Through the RIG-I Pathway Using TRITC-Dextran Encapsulated Nanoparticles. Immune Netw. 2024 Dec 24;24(6):e44.
[5] Xia ZY, Luo C, Liu BW, et al. Shengui Sansheng Pulvis maintains blood-brain barrier integrity by vasoactive intestinal peptide after ischemic stroke. Phytomedicine. 2020 Feb;67:153158.
[6] Choi AR, Jung MJ, Kim JH, et al. Co-treatment of Salinomycin Sensitizes AZD5363-treated Cancer Cells Through Increased Apoptosis. Anticancer Res. 2015 Sep;35(9):4741-7.
[7] Tanaka M, Tanaka K, Masaki Y, et al. Intrahepatic microcirculatory disorder, parenchymal hypoxia and NOX4 upregulation result in zonal differences in hepatocyte apoptosis following lipopolysaccharide- and D-galactosamine-induced acute liver failure in rats. Int J Mol Med. 2014 Feb;33(2):254-62.
[8] Hakim JS, Rodysill BR, Chen BK, et al. Combinatorial tissue engineering partially restores function after spinal cord injury. J Tissue Eng Regen Med. 2019 May;13(5):857-873.

TRITC-dextran, MW 70000是一种用于追踪和研究细胞过程（如内吞、外排和细胞标记）的荧光标记右旋糖酐，最大吸收光/发射光波长为550/571nm，具有高稳定性和抗光漂白性^[1]。TRITC-dextran在生物研究中被广泛用于标记细胞或细胞内区域并监测各种生物活动^[2]，TRITC-dextran还能用于标记神经元和神经网络，深入研究神经递质的释放与传递机制^[3]。此外，TRITC-dextran可以作为改变纳米颗粒表面特性的封装材料^[4]。

在体外，在下室接种单层细胞的HT29的Transwells上室中添加TRITC-dextran（10μg/ml），37°C下孵育1-4小时，随后测量下室的TRITC-dextran的荧光强度来评估细胞单层的紧密连接的通透性^[5]。将 TRITC-dextran（2mg/ml）加入到在下室接种BMECs的Transwells上室中，通过检测BMECs中TRITC-dextran的荧光强度来评估血脑屏障的完整性^[6]。

在体内，TRITC-dextran可被用于评估大鼠肝脏微循环，使用TRITC-dextran（500mg/kg）通过静脉注射的方式处理大鼠，在处理后1分钟，切除肝脏以获取组织样本，将肝脏组织固定在 20% 甲醛溶液中，进行石蜡包埋和切片切片经过脱蜡、水化、磷酸盐缓冲液（PBS）洗涤后，立即用含水的Fluor Mount/plus封片剂封片，以便在荧光显微镜下观察TRITC-dextran的分布^[7]。TRITC-dextran可被用于评估评估脊髓损伤后再生组织的血管灌注情况。通过小鼠尾静脉注射250μl的生理盐水溶液，其中含有10mg/ml的TRITC-dextran，通过测量纵向图像视野内荧光的表面积来定量评估TRITC-dextran的含量，以检测血管与系统循环的情况^[8]。