TOTO 1 Green Fluorescent Dye (5 mM in DMSO)

TOTO 1 is an excellent nuclear and chromosomal counterstain that cannot penetrate live cells, but can penetrate dead cells with damaged membrane features, making it a useful dead cell indicator within cell populations. TOTO 1 has almost no fluorescence in an environment without nucleic acid. Once it binds to nucleic acid, it produces bright green fluorescence with maximum excitation and emission wavelengths of~514/531nm, respectively

Important reminder (before purchase or initial use): Please make sure to refer to:
1、 Precautions for preparing storage solutions of fluorescent dyes (in powder form, especially oxygen sensitive probes)
1) Fluorescent probes are very stable in solid (powder) state and can be stored at the required temperature according to the instructions. They can be used within the expiration date.
2) Fluorescent probes are dissolved in organic solvents such as DMSO and prepared into storage solutions. Generally, they are stored at -20 ° C (for use within 2-3 months, or even shorter, please consult for details); Store at -80 ° C (for use within 6 months, please consult). The premise is that the organic solvents used must be of high quality and anhydrous, especially DMSO, which must be freshly opened.
3) The prepared storage solution must be stored in a low capacity cryovial with good sealing and a screw cap (EP tubing is not allowed), and at least 5-10 U I/tube should be divided and stored away from light.
4) For certain special compounds (sensitive to air or with unstable structures), the storage period may be particularly short, and they can only be used on the same day. These compounds will be explained in the instruction manual.
For more information, please contact our staff to verify.
2、 Precautions for fluorescent dyes (provided in the form of storage solutions dissolved in organic solvents)
1) Fluorescent probes in the form of storage solutions dissolved in organic solvents are relatively stable compounds; But when receiving such products, users also need to pack them according to the single dosage (5-10ul/tube) and store them in a sealed and dark place below -20 ℃ to reduce the number of repeated freeze-thaw cycles.
2) Please make sure to store in a low capacity cryovial with good sealing and screw cap (EP tubing is not acceptable), and avoid light exposure.
For more information, please contact our staff to verify.

TOTO 1是一种优秀的细胞核和染色体复染剂，不能穿透活细胞，但能渗透进入具受损膜特征的死细胞，使其在细胞群内是一种有用的死细胞指
示剂。TOTO 1在不含核酸的环境下基本无荧光，一旦与核酸结合产生明亮的绿色荧光，最大激发和发射波长分别为~514/531nm.

重要提醒(购买或初次使用前)请务必查阅:
一、荧光染料(粉末形式，特别是对氧敏感探针)配制储存液的注意事项
1)荧光探针在固体(粉末)状态很稳定，按照说明书要求温度来保存，效期内使用即可。
2)荧光探针用有机溶剂比如:DMSO，溶解配制成储存液之后，一般来说，放到-20℃保存(2-3个月内使用，甚至更短，具体可咨询);放到-80°C保存(6个月内使用，具体可咨询)。前提是，使用的有机溶剂必须是高质量且无水的，特别是DMSO，必须是新鲜开封。
3)配制的储存液请务必用密封性好且螺旋盖的低容量冻存管保存(不可用EP管)，至少按照5-10u I/管来分装，避光冻存。
4)对于某些特殊化合物(对空气敏感或存在不稳定结构)，可能保存周期特别短，甚至只能当天使用。这些化合物说明书上会有说明。
有更多信息，请联系我司工作人员来核实。
二、荧光染料 (以溶于有机溶剂的储存液形式提供)的注意事项
1) 以溶于有机溶剂的储存液形式的荧光探针相对来说是比较稳定的化合物;但收到这类产品，也需用户根据单次用量(5-10ul/管来分装)，-20℃以下密封避光保存，减少反复冻融次数。
2) 请务必用密封性好且螺旋盖的低容量冻存管保存(不可用EP管)。务必避光。
有更多信息，请联系我司工作人员来核实。