TNC Protein, Human (His)
目录号 : GC27166TNC 蛋白引导神经元和轴突迁移，并有助于发育、突触可塑性和神经元再生。TNC 蛋白, Human (His) 是重组的 TNC 蛋白，由 E. coli 表达，带有 N-6*His 标签。
Sample solution is provided at 25 µL, 10mM.
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TNC 蛋白是一种细胞外基质蛋白，在发育过程中引导神经元和轴突迁移方面发挥着至关重要的作用，有助于突触可塑性和神经元再生等过程。它促进单层星形胶质细胞上生长的皮层神经元的神经突生长，表明它参与神经系统内复杂的细胞相互作用。作为整合素 α-8/β-1、α-9/β-1、α-V/β-3 和 α-V/β-6 的配体，TNC 建立了细胞通讯和信号传导所必需的分子连接。在肿瘤中，TNC 通过促进内皮细胞的伸长、迁移和萌芽来刺激血管生成。在结构上，TNC 以同源六聚体形式存在，在三卷曲螺旋区域可能形成同源三聚体，并通过两端的二硫环进一步稳定。这种多功能蛋白质还与 CSPG4 相互作用，表明它参与不同生物背景下的多种细胞和分子过程。
| Purity | Greater than 95% as determined by reducing SDS-PAGE. | Source | E. coli |
| Phycical Appearance | Lyophilized powder | Shipping Condition | |
| Synonyms | TNC 蛋白, Human (His); Cytotactin; Glioma associated extracellular matrix antigen; GMEM; TenascinC | ||
| Amino Acid Sequence | DSPRDLTATEVQSETALLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDTTSYSLADLSPSTHYTAKIQALNGPLRSNMIQTIFTTIGLLYPFPKDCSQAMLNGDTTSGLYTIYLNGDKAEALEVFCDMTSDGGGWIVFLRRKNGRENFYQNWKAYAAGFGDRREEFWLGLDNLNKITAQGQYELRVDLRDHGETAFAVYDKFSVGDAKTRYKLKVEGYSGTAGDSMAYHNGRSFSTFDKDTDSAITNCALSYKGAFWYRNCHRVNLMGRYGDNNHSQGVNWFHWKGHEHSIQFAEMKLRPSNFRNLEGRRKRA | ||
| Apparent Molecular Weight | Approximately 36-39.5 kDa | ||
| Stability | Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. | ||
| Biological Activity | Measured by the ability of the immobilized protein to block Fibronectin-mediated adhesion of NIH-3T3 mouse embryonic fibroblast cells. rhTenascin-C immobilized at 15 μg/mL, in the presence of 0.1 μg/mL human Fibronectin, will block approximately 54.61% NIH3/T3 cell adhesion (5x104 cells/well, 100 μL/well) | ||
| Endotoxin Level | <1 EU/μg,determined by LAL method. | ||
| Reconstitution | It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose). | ||
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4 or 10 mM Tris-HCI,1 mM EDTA, 6% Trehalose,pH 8.0 or 50 mM Tris-HCI.300 mM NaCl,pH 7.4. | ||
TNC proteins guide neuronal and axonal migration and contribute to development, synaptic plasticity, and neuronal regeneration. TNC Protein, Human (His) is the recombinant human-derived TNC protein, expressed by E. coli , with N-6*His labeled tag.
Measured by the ability of the immobilized protein to block Fibronectin-mediated adhesion of NIH-3T3 mouse embryonic fibroblast cells. rhTenascin-C immobilized at 15 μg/mL, in the presence of 0.1 μg/mL human Fibronectin, will block approximately 54.61% NIH3/T3 cell adhesion (5x104 cells/well, 100 μL/well)
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.