Tis108
目录号 : GC19278
Tis108是一种三唑类三唑类独脚金内酯（SL）生物合成抑制剂。
Cas No.:1315459-30-3
Sample solution is provided at 25 µL, 10mM.
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Tis108 is a triazole-type strigolactone (SL)-biosynthesis inhibitor[1].
TIS108 (3μM) increased the number of branches in Arabidopsis and inhibited root hair elongation[1].Tis108 TIS108 (0, 0.2μM, 1μM and 5μM) has been confirmed to enhance the shoot regeneration capacity of woody plants in M26 material (Malus pumila Mill., a type of apple rootstock)[2]. Tis108 (10μM; 5, 10 and 30 days) promotes stem growth and development of cherry rootstocks and increases the size of cortical cells in cherry rootstocks[3].Treatment of rice seedlings with Tis108 (10-100nM) reduces the levels of strigolactones (SL) in both roots and root exudates in a concentration-dependent manner. The root exudates from Tis108 (1μM)-treated rice seedlings have a weaker stimulatory effect on the germination of Striga compared to those from control plants[4]. Tis108 (3μM; 3 weeks) decreased cotton resistance to Verticillium wilt[5].
References:
[1] Ito S, Umehara M, Hanada A, Yamaguchi S, Asami T. Effects of strigolactone-biosynthesis inhibitor TIS108 on Arabidopsis. Plant Signal Behav. 2013 May;8(5):e24193.
[2] Asghar S, Xiong Y, Che M, Fan X, Li H, Wang Y, Xu X, Li W, Han Z. Transcriptome analysis reveals the effects of strigolactone on shoot regeneration of apple. Plant Cell Rep. 2022 Jul;41(7):1613-1626.
[3] Liu X, Xu Y, Sun W, Wang J, Gao Y, Wang L, Xu W, Wang S, Jiu S, Zhang C. Strigolactones modulate stem length and diameter of cherry rootstocks through interaction with other hormone signaling pathways. Front Plant Sci. 2023 Feb 9;14:1092654.
[4] Ito S, Umehara M, Hanada A, Kitahata N, Hayase H, Yamaguchi S, Asami T. Effects of triazole derivatives on strigolactone levels and growth retardation in rice. PLoS One. 2011;6(7):e21723.
[5] Yi F, Song A, Cheng K, Liu J, Wang C, Shao L, Wu S, Wang P, Zhu J, Liang Z, Chang Y, Chu Z, Cai C, Zhang X, Wang P, Chen A, Xu J, Burritt DJ, Herrera-Estrella L, Tran LP, Li W, Cai Y. Strigolactones positively regulate Verticillium wilt resistance in cotton via crosstalk with other hormones. Plant Physiol. 2023 May 31;192(2):945-966.
Tis108是一种三唑类三唑类独脚金内酯（SL）生物合成抑制剂[1]。
Tis108（3μM）增加了拟南芥的分枝数量并抑制了根毛的伸长[1]。Tis108(0, 0.2 μM, 1μM和5μM)已被证实可以提高M26材料(苹果砧木, Malus pumila Mill.)中木本植物的茎段再生能力[2]。Tis108(10μM; 5, 10和30天)促进了樱桃砧木的茎生长和发育，并增加了樱桃砧木皮层细胞的大小[3]。用Tis108(10-100nM)处理水稻幼苗会以浓度依赖的方式降低根和根分泌物中的油菜素内酯（SL）水平。与对照植株相比，经1μM Tis108处理的水稻幼苗的根分泌物对独脚金萌发的刺激作用较弱[4]。Tis108(3μM; 3周)降低了棉花对黄萎病的抗性[5]。
| Plant experiment [1]: | |
Plant models | Hai7124 |
Preparation Method | Twenty-four hours prior to inoculation with V.dahliae spores, cotton seedlings (Hai7124) were subjected to soil drenching with 5mL of Tis108 solution (3μM) or an equal volume of water (containing an equivalent amount of ethanol). During the infection period, Tis108 or mock control treatment was applied twice a week to maintain the effect of Tis108. Three weeks after V.dahliae infection, Hai7124 cotton seedlings treated with Tis108 exhibited more severe disease symptoms compared with mock control plants. The disease index of Tis108-treated plants increased by approximately 20% compared with the control group. |
Dosage form | 3μM; 3 weeks |
Applications | Tis108 (3μM; 3 weeks) decreased cotton resistance to Verticillium wilt. |
References: | |
| Cas No. | 1315459-30-3 | SDF | |
| 化学名 | 6-phenoxy-1-phenyl-2-(1H-1,2,4-triazol-1-yl)hexan-1-one | ||
| 分子式 | C20H21N3O2 | 分子量 | 335.40 |
| 溶解度 | DMSO : ≥ 125 mg/mL (372.69 mM) | 储存条件 | Store at -20℃ |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.9815 mL | 14.9076 mL | 29.8151 mL |
| 5 mM | 0.5963 mL | 2.9815 mL | 5.963 mL |
| 10 mM | 0.2982 mL | 1.4908 mL | 2.9815 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet