Sucrose (D-(+)-Saccharose)
(Synonyms: 蔗糖; D-(+)-Saccharose) 目录号 : GC31352
Sucrose (D-(+)-Saccharose), a common sugar produced naturally in plants, is a disaccharide, a molecule composed of two monosaccharides:glucose and fructose.
Cas No.:57-50-1
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Animal experiment: | A total of 66 male 8-wk-old Sprague-Dawley obesity-prone (OP) and obesity-resistant (OR) rats (n = 38 per each phenotype), weighing 275 g and 210 g respectively, are used in this study. After recovery and attaining pre-surgical weights, rats undergo three training trials of 60-min sham feeding sessions with 0.03 M Sucrose solution. During testing, rats are briefly deprived of water (0900 to 1600 h) but not food, and are tested for 1 h (1400 to1500 h) sham intake of 0.03 M Sucrose solution until a stable baseline is achieved. Afterwards, rats are sham fed one of three Sucrose solutions (0.03, 0.3, and 1.0 M) in random order, every other day, with each concentration tested at least twice. Sucrose intake is individually recorded every 5 min for 60 min[1]. |
References: [1]. Duca FA, et al. Effect of diet on preference and intake of sucrose in obese prone and resistant rats. PLoS One. 2014 Oct 20;9(10):e111232. | |
Sucrose (D-(+)-Saccharose), a common sugar produced naturally in plants, is a disaccharide, a molecule composed of two monosaccharides:glucose and fructose.
[1] C Foyer, et al. Arch Biochem Biophys. 1983 Jan;220(1):232-8.
| Cas No. | 57-50-1 | SDF | |
| 别名 | 蔗糖; D-(+)-Saccharose | ||
| Canonical SMILES | O[C@H]1[C@H](O)[C@@H](CO)O[C@H](O[C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@@H]1O | ||
| 分子式 | C12H22O11 | 分子量 | 342.3 |
| 溶解度 | Water : 100 mg/mL (292.14 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.9214 mL | 14.6071 mL | 29.2141 mL |
| 5 mM | 0.5843 mL | 2.9214 mL | 5.8428 mL |
| 10 mM | 0.2921 mL | 1.4607 mL | 2.9214 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Sucrose signaling in higher plants
Plant Sci.2021 Jan;302:110703.PMID:33288016DOI: 10.1016/j.plantsci.2020.110703.
Sucrose controls various developmental and metabolic processes in plants. In this review, we evaluate whether sucrose could be a preferred signaling molecule that controls processes like carbohydrate metabolism, accumulation of storage proteins, sucrose transport, anthocyanin accumulation, and floral induction. We summarize putative sucrose-dependent signaling pathways. Sucrose, but not other sugars, stimulates the genes that encode ADP-glucose pyrophosphorylase (AGPase), granule-bound starch synthase I, and UDP-glucose pyrophosphorylase in several species. The class-1 patatin promoter is induced under high sucrose conditions in potato (Solanum tuberosum). Exogenous sucrose reduces the loading of sucrose to the phloem by inhibiting the expression of the sucrose transporter and its protein activity in sugar beet (Beta vulgaris). Sucrose also influences a wide range of growth processes, including cell division, ribosome synthesis, cotyledon development, far-red light signaling, and tuber development. Floral induction is promoted by sucrose in several species. The molecular mechanisms by which sucrose functions as a signal are largely unknown. Sucrose enhances the expression of transcription factors such as AtWRKY20 and MYB75, which function upstream of the sucrose-responsive genes. Sucrose controls the expression of AtbZIP11 at the post-transcriptional level by the peptide encoded by uORF2. Sucrose levels affect translation of a group of mRNAs in Arabidopsis. Sucrose increases the activity of AGPase by posttranslational redox-modification. Sucrose interrupts the interaction between sucrose transporter SUT4 and cytochrome b5. In addition, the SNF-related protein kinase-1 appears to be involved in sucrose-dependent pathways by controlling sucrose synthase (SUS4) expression.
Sucrose transporter in rice
Plant Signal Behav.2021 Nov 2;16(11):1952373.PMID:34269147DOI: 10.1080/15592324.2021.1952373.
Plant photosynthesis processes play vital roles in crop plant development. Understanding carbohydrate partitioning via sugar transport is one of the potential ways to modify crop biomass, which is tightly linked to plant architecture, such as plant height and panicle size. Based on the literature, we highlight recent findings to summarize phloem loading by sucrose transport in rice. In rice, sucrose transporters, OsSUTs (sucrose transporters) and OsSWEETs (sugars are eventually exported transporters) import sucrose and export cells between phloem parenchyma cells and companion cells. Before sucrose transporters perform their functions, several transcription factors can induce sucrose transporter gene transcription levels, such as Oryza sativa DNA binding with one finger 11 (OsDOF11) and Oryza sativa Nuclear Factor Y B1 (OsNF-YB1). In addition to native regulator genes, environmental factors, such as CO2 concentration, drought stress and increased temperature, also affect sucrose transporter gene transcription levels. However, more research work is needed on formation regulation webs. Elucidation of the phloem loading mechanism could improve our understanding of rice development under multiple conditions and facilitate its manipulation to increase crop productivity.
Phloem Loading and Unloading of Sucrose: What a Long, Strange Trip from Source to Sink
Annu Rev Plant Biol.2022 May 20;73:553-584.PMID:35171647DOI: 10.1146/annurev-arplant-070721-083240.
Sucrose is transported from sources (mature leaves) to sinks (importing tissues such as roots, stems, fruits, and seeds) through the phloem tissues in veins. In many herbaceous crop species, sucrose must first be effluxed to the cell wall by a sugar transporter of the SWEET family prior to being taken up into phloem companion cells or sieve elements by a different sugar transporter, called SUT or SUC. The import of sucrose into these cells is termed apoplasmic phloem loading. In sinks, sucrose can similarly exit the phloem apoplasmically or, alternatively, symplasmically through plasmodesmata into connecting parenchyma storage cells. Recent advances describing the regulation and manipulation of sugar transporter expression and activities provide stimulating new insights into sucrose phloem loading in sources and unloading processes in sink tissues. Additionally, new breakthroughs have revealed distinct subpopulations of cells in leaves with different functions pertaining to phloem loading. These and other discoveries in sucrose transport are discussed.
Comprehensive utilization of sucrose resources via chemical and biotechnological processes: A review
Biotechnol Adv.2022 Nov;60:107990.PMID:35640819DOI: 10.1016/j.biotechadv.2022.107990.
Sucrose, one of the most widespread disaccharides in nature, has been available in daily human life for many centuries. As an abundant and cheap sweetener, sucrose plays an essential role in our diet and the food industry. However, it has been determined that many diseases, such as obesity, diabetes, hyperlipidemia, etc., directly relate to the overconsumption of sucrose. It arouses many explorations for the conversion of sucrose to high-value chemicals. Production of valuable substances from sucrose by chemical methods has been studied since a half-century ago. Compared to chemical processes, biotechnological conversion approaches of sucrose are more environmentally friendly. Many enzymes can use sucrose as the substrate to generate functional sugars, especially those from GH68, GH70, GH13, and GH32 families. In this review, enzymatic catalysis and whole-cell fermentation of sucrose for the production of valuable chemicals were reviewed. The multienzyme cascade catalysis and metabolic engineering strategies were addressed.
Sucrose signaling in plants: a world yet to be explored
Plant Signal Behav.2013 Mar;8(3):e23316.PMID:23333971DOI: 10.4161/psb.23316.
The role of sucrose as a signaling molecule in plants was originally proposed several decades ago. However, recognition of sucrose as a true signal has been largely debated and only recently this role has been fully accepted. The best-studied cases of sucrose signaling involve metabolic processes, such as the induction of fructan or anthocyanin synthesis, but a large volume of scattered information suggests that sucrose signals may control a vast array of developmental processes along the whole life cycle of the plant. Also, wide gaps exist in our current understanding of the intracellular steps that mediate sucrose action. Sucrose concentration in plant tissues tends to be directly related to light intensity, and inversely related to temperature, and accordingly, exogenous sucrose supply often mimics the effect of high light and cold. However, many exceptions to this rule seem to occur due to interactions with other signaling pathways. In conclusion, the sucrose role as a signal molecule in plants is starting to be unveiled and much research is still needed to have a complete map of its significance in plant function.