Streptavidin Agarose 6FF

GLPBIO Streptavidin Agarose 6FF, a 6% highly cross-linked agarose reagent coupled with recombinant streptavidin, is an affinity chromatography medium for separation and purification of biotinylated peptides, antibodies, lectins, etc. The total binding capacity of Streptavidin Agarose 6FF is more than 200 nmol of D-Biotin/mL settled resin.

Streptavidin, isolated from Streptomyces avidinii, binds 4 moles of biotin per mole of protein with an extremely high affinity. With no carbohydrate group and an isoelectric point of 6.5 (different from avidin), streptavidin has less nonspecific binding. Streptavidin has a strong affinity with biotin and needs to be eluted under denaturing conditions when purified. However, streptavidin has a relatively weak affinity for iminobiotin, which binds at pH 9.5-11.0 and elutes at pH 4.0 without the use of denaturing agents.

Characteristics

The specifications of the product correspond to the actual resin volume, with the resin content of 50%.

GLPBIO 链霉亲和素琼脂糖 6FF 以高度交联的 6% 琼脂糖凝胶为基质，通过化学方法共价偶联了重组链霉亲和素，结合能力更强，每毫升介质可结合 200 nmol Biotin。

链霉亲和素 (Streptavidin) 最早发现于阿维丁链霉菌 (Streptomyces avidinii) 的培养液中，是一种四聚体蛋白质。含有四个生物素 (D-Biotin) 结合位点，但其不含碳水化合 物侧链，具有近中性的等电点 6.5 (亲和素为 10)，更有利于生物反应进行，因此链霉亲和素特异性结合能力更强。

特性

本产品的规格与实际凝胶体积一致，凝胶含量为 50%。