Soyasaponin Bb
(Synonyms: 大豆皂苷Bb) 目录号 : GN10234
Soyasaponin BB是一种从豆科植物大豆中提取的三萜类齐墩果酸型皂，是一种口服有效的活性分子，兼具诱导血红素加氧酶-1(HO-1)表达与抑制醛糖还原酶AKR1B1的双重作用。
Cas No.:51330-27-9
Sample solution is provided at 25 µL, 10mM.
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Soyasaponin BB is an oleanane-type triterpenoid saponin derived from soybeans (a leguminous plant). As an orally active molecule, Soyasaponin BB exhibits a dual mechanism of action by inducing the expression of heme oxygenase-1 (HO-1) while simultaneously inhibiting the aldose reductase AKR1B1[1-2]. Soyasaponin BB helps reduce inflammatory responses and promotes cartilage tissue regeneration[3]. Soyasaponin BB can also alleviate LPS-induced cognitive dysfunction and intestinal inflammation[4].
In vitro, when C2C12 cells were co-cultured with Soyasaponin BB (10–30μg/mL) for 3 days under the induction of bone morphogenetic protein-2 (BMP-2; 100ng/mL), Soyasaponin BB promoted their differentiation into osteoblasts and significantly enhanced the activity and expression of alkaline phosphatase (ALP). Soyasaponin BB activates the AKT/MAPK signaling pathway, thereby promoting the transcription of downstream osteogenic markers such as osteocalcin (OCL) and type I collagen (Col1a)[5]. Pretreatment of BRL 3A rat hepatocytes with Soyasaponin BB (25–100μg/mL) for 24 hours, followed by stimulation with ethanol (250mmol/L) for another 24 hours, significantly alleviated ethanol-induced oxidative stress and hepatocyte damage[6].
In vivo, intragastric administration of Soyasaponin BB (10mg/kg; for 4 consecutive days) to male C57BL mice undergoing spontaneous withdrawal after chronic intermittent escalating-dose morphine administration significantly reduced withdrawal-induced anxiety-like behaviors[7]. Dietary supplementation with Soyasaponin BB (0.2g/100g feed) for 90 days in 60-day-old male pcy mice markedly delayed the progression of polycystic kidney disease[8].
References:
[1] Zhang Y, Chen F, Chen J, et al. Soyasaponin Bb inhibits the recruitment of toll-like receptor 4 (TLR4) into lipid rafts and its signaling pathway by suppressing the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-dependent generation of reactive oxygen species. Mol Nutr Food Res. 2016 Jul;60(7):1532-43.
[2] Lin X, Zhang C, Huang B. Hepatoprotective action mechanism and quantification of soyasaponin Bb in Abri Herba by HPLC and network pharmacology. J Ethnopharmacol. 2025 Jan 30;337(Pt 2):118850.
[3] Jiang Y, Li T, Liu B, et al. Soyasaponin Bb/Gelatin-Methacryloyl Hydrogel for Cartilage Inflammation Inhibition. ACS Omega. 2024 Dec 4;9(50):49597-49608.
[4] Yun SW, Lee DY, Park HS, et al. Heat-Processed Soybean Germ Extract and Lactobacillus gasseri NK109 Supplementation Reduce LPS-Induced Cognitive Impairment and Colitis in Mice. Nutrients. 2024 Aug 16;16(16):2736. doi: 10.3390/nu16162736.
[5] Kim SH, Yuk HJ, Ryu HW, et al. Biofunctional soyasaponin Bb in peanut (Arachis hypogaea L.) sprouts enhances bone morphogenetic protein-2-dependent osteogenic differentiation via activation of runt-related transcription factor 2 in C2C12 cells. Phytother Res. 2019 May;33(5):1490-1500.
[6] Lijie Z, Ranran F, Xiuying L, et al. Soyasaponin Bb Protects Rat Hepatocytes from Alcohol-Induced Oxidative Stress by Inducing Heme Oxygenase-1. Pharmacogn Mag. 2016 Oct-Dec;12(48):302-306.
[7] Wu J, Zhu C, Shu Y, et al. Soyasaponin Bb Attenuates Morphine Withdrawal-Induced Anxiety-Like Behaviors in Male Mice by Rescuing GSK-3β/Nrf2 Signaling. Psychiatry Investig. 2025 Jul;22(7):821-831.
[8] Philbrick DJ, Bureau DP, Collins FW, et al. Evidence that soyasaponin Bb retards disease progression in a murine model of polycystic kidney disease. Kidney Int. 2003 Apr;63(4):1230-9.
Soyasaponin BB是一种从豆科植物大豆中提取的三萜类齐墩果酸型皂，是一种口服有效的活性分子，兼具诱导血红素加氧酶-1(HO-1)表达与抑制醛糖还原酶AKR1B1的双重作用[1-2]。Soyasaponin BB有利于降低炎症反应，并促进软骨组织再生[3]。Soyasaponin BB可减轻LPS诱导的认知功能障碍和肠道炎症[4]。
在体外，Soyasaponin BB（10–30μg/mL）与C2C12细胞共培养3天，同时在骨形态发生蛋白-2（BMP-2；100ng/mL）诱导下促进其向成骨细胞分化，显著增强碱性磷酸酶（ALP）的活性和表达。Soyasaponin BB可激活AKT/MAPK信号通路，进而促进下游成骨标志分子如骨钙素（OCL）和Ⅰ型胶原（Col1a）的转录水平[5]。Soyasaponin BB（25–100μg/mL）预处理BRL 3A大鼠肝细胞24小时，随后以乙醇（250mmol/L）刺激24小时，Soyasaponin BB显著减轻了乙醇诱导的氧化应激和肝细胞损伤 [6]。
在体内，Soyasaponin BB（10mg/kg；连续4天）通过胃内给药处理经历慢性间歇性递增剂量吗啡给药后自发戒断的雄性C57BL小鼠，Soyasaponin BB显著减轻了戒断诱导的焦虑样行为[7]。Soyasaponin BB（0.2g/100g饲料）通过膳食补充方式处理60日龄雄性pcy小鼠90天，显著延缓了多囊肾病的疾病进展[8]。
| Cell experiment [1]: | |
Cell lines | C2C12 cells (mouse mesenchymal precursor cell line) |
Preparation Method | C2C12 cells were maintained in alpha minimum essential medium (α-MEM) supplemented with 10% fetal bovine serum (FBS), 100U/mL penicillin, and 100μg/mL streptomycin at 37°C, 5% CO₂. For osteogenic differentiation, cells were treated with bone morphogenetic protein-2 (BMP-2; 100ng/mL) and Soyasaponin BB at concentrations of 10–30μg/mL for 3 days. |
Reaction Conditions | 10–30μg/mL; 3 days |
Applications | Soyasaponin BB significantly enhanced BMP-2-induced alkaline phosphatase (ALP) activity and promoted osteoblast differentiation in a dose-dependent manner. Soyasaponin BB upregulated the mRNA and protein expression of osteogenic markers, including runt-related transcription factor 2 (Runx2), osteocalcin (OCL), and collagen type I alpha (Col1a). Soyasaponin Bb also activated AKT and MAPK signaling pathways but did not affect Smad phosphorylation. |
| Animal experiment [2]: | |
Animal models | C57BL mice |
Preparation Method | Mice were subjected to a chronic intermittent escalating-dose morphine administration paradigm (20-100mg/kg; i.p.; twice daily for 7 days) followed by spontaneous withdrawal. Soyasaponin Bb (10mg/kg; for 4 days) was administered intragastrically during the withdrawal period. |
Dosage form | 10mg/kg; intragastric administration; for 4 days |
Applications | Soyasaponin BB significantly attenuated morphine withdrawal-induced anxiety-like behaviors. Soyasaponin BB rescued the GSK-3β/Nrf2 signaling pathway in the basolateral amygdala (BLA) by reducing GSK-3β activity and enhancing Nrf2 and SOD expression. |
References: | |
| Cas No. | 51330-27-9 | SDF | |
| 别名 | 大豆皂苷Bb | ||
| 化学名 | Soyasaponin BB | ||
| Canonical SMILES | CC1C(C(C(C(O1)OC2C(C(C(OC2OC3C(C(C(OC3OC4CCC5(C(C4(C)CO)CCC6(C5CC=C7C6(CCC8(C7CC(CC8O)(C)C)C)C)C)C)C(=O)O)O)O)CO)O)O)O)O)O | ||
| 分子式 | C48H78O18 | 分子量 | 943.12 |
| 溶解度 | ≥ 81.8mg/mL in DMSO | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.0603 mL | 5.3016 mL | 10.6031 mL |
| 5 mM | 212.1 μL | 1.0603 mL | 2.1206 mL |
| 10 mM | 106 μL | 530.2 μL | 1.0603 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >98.00%
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