Rose Bengal
(Synonyms: 酸性红 94) 目录号 : GC15691
A xanthene dye, fluorescein derivative, and photosensitizer
Cas No.:632-69-9
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Ki: 19 and 64 nM for VGlut andVMAT, respectively
Rose Bengal is a potent VGlut and vesicular monoamine transporter (VMAT) inhibitor.
The common biochemical l-glutamate (Glu) is widely recognized as the major excitatory neurotransmitter in the vertebrate CNS. Proper Glu transmission plays a critical role in physiological brain functions, such as learning and memory formation as well as basic neural communication.
In vitro: Rose Bengal was a quite potent membrane-permeant inhibitor of glutamate uptake into isolated synaptic vesicles. Such vesicular Glu uptake inhibition was achieved without affecting H1-pump ATPase. It was found that various degrees of reduction elicited by Rose Bengal in [3H]Glu in synaptic vesicles inside the synaptosome [1].
In vivo: The distribution of i.v. administered rose bengal was found to depend on its dose. At a low dose, rose bengal could be found almost solely in the liver and plasma. However, at higher doses, the amount of rose bengal found in extra-hepatic tissues gradually increased. The hepatic transfer maximum of rose bengal amounted to 146 micrograms/kg/min. By increasing the dose from 10 to 200 mg/kg, the hepatic concentration of rose bengal also approached a maximum. The storage capacity of the liver, however, did not limit the transfer maximum of rose bengal [1].
Clinical trial: N/A
References:
[1] Ogita K,Hirata K,Bole DG,Yoshida S,Tamura Y,Leckenby AM,Ueda T. Inhibition of vesicular glutamate storage and exocytotic release by Rose Bengal. J Neurochem.2001 Apr;77(1):34-42.
[2] Fischer E,Varga F. Hepatic storage and biliary excretion of rose bengal in the rat. Acta Physiol Acad Sci Hung.1979;54(1):89-94.
| Cas No. | 632-69-9 | SDF | |
| 别名 | 酸性红 94 | ||
| 化学名 | sodium 2,3,4,5-tetrachloro-6-(2,4,5,7-tetraiodo-6-oxido-3-oxo-3H-xanthen-9-yl)benzoate | ||
| Canonical SMILES | IC1=C(C(C(C(C(C([O-])=O)=C2Cl)=C(C(Cl)=C2Cl)Cl)=C3C=C4I)=CC(I)=C1[O-])OC3=C(C4=O)I.[Na+].[Na+] | ||
| 分子式 | C20H2Cl4I4Na2O5 | 分子量 | 1017.64 |
| 溶解度 | <101.76mg/ml in Water; <50.88mg/ml in DMSO | 储存条件 | Store at RT, protect from light, stored under nitrogen |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.9827 mL | 4.9133 mL | 9.8267 mL |
| 5 mM | 0.1965 mL | 0.9827 mL | 1.9653 mL |
| 10 mM | 0.0983 mL | 0.4913 mL | 0.9827 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。