RNase Inhibitor, Murine
目录号 : GK12001鼠源Rnase抑制剂（RNase Inhibitor, Murine）, 是一个50 kD的重组蛋白，从大肠杆菌中分离出来，含有来自小鼠的核糖核酸酶抑制剂基因。
Sample solution is provided at 25 µL, 10mM.
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1. Used in IVT(In vitro translation), RT-PCR, cDNA synthesis and other application Add RNase Inhibitor, Murine to achieve a final concentration of 1U/µl in the reaction. Be careful that RNase Inhibitor should be added before other components that may possibly containing RNase (i.e. enzymes, plasmid) Murine RNase Inhibitor is a 50 kDa protein that will be inactivated in denaturing conditions such as high temperature and denaturing agents, it must be used below 50°C.
2. Used in adding poly(A) tail to RNA In the experiment of adding poly(A) tail to RNA, RNase Inhibitor can be added to enhance the stability of the RNA. 0.5 µl of RNase inhibitor to a 20 µl reaction is appropriate when the reaction is set-up. The additional volume can be subtracted from the amount of H2O used in the reaction.
RNase Inhibitor, Murine is a 50 kD recombinant protein isolated from E. coli containing the ribonuclease inhibitor gene originally from mouse. Our product RNase inhibitor has specificity for RNases A, B and C, but couldn’t inhibit RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. It can bind several kinds of RNase non-covalently in a 1:1 ratio with high affinity. Especially, according to previous researches, no inhibition for enzyme activity is observed when RNase Inhibitor is used within AMV or M-MuLV reverse transcriptases, taq DNA polymerase or SP6/ T7/ T3 RNA polymerases, means it can be taken in various experiments to prevent RNA degradation.
Compared to RNase from human, RNase inhibitor from mouse lacks the pair of cysteines which is very sensitive to oxidation and lead to inactivation of the inhibitor. As a consequence, RNase Inhibitor, Murine significantly improves its resistance to oxidation compared to RNase inhibitors from human/porcine. It is stable at low DTT concentrations (less than 1 mM), and this character makes it preferred for reactions where low concentration DTT is necessary to the reaction (eg. Real-time RT-PCR).
Our product can be used in many kinds of reactions to prevent RNA degradation, for example, RT-PCR, cDNA synthesis, In vitro transcription, enzymatic RNA labeling reaction or any other applications.
RNase抑制剂（Murine）是一种从大肠杆菌中分离出来的50 kD重组蛋白，含有最初来自小鼠的核糖核酸酶抑制剂基因。我们的RNase抑制剂对RNase A、B和C具有特异性，但无法抑制RNase 1、RNase T1、S1核酸酶、RNase H或来自曲霉属的RNase。它可以非共价地与几种RNase结合成1:1的比例，并具有高亲和力。特别地，在以前的研究中，发现RNase抑制剂在AMV或M-MuLV逆转录酶、Taq DNA聚合酶或SP6 / T7 / T3 RNA聚合酶中使用时不会抑制酶活性，这意味着它可以在各种实验中使用以防止RNA降解[1,2]。
与来自人体的RNase相比，来自小鼠的RNase抑制剂缺少一对对氧化非常敏感的半胱氨酸，这会导致抑制剂失活。因此，与来自人类/猪的RNase抑制剂相比，RNase抑制剂（Murine）显着提高了对氧化的抵抗力。它在低DTT浓度（小于1 mM）下稳定，这使它成为需要低浓度DTT参与反应的实验的首选（例如实时RT-PCR）[2]。
我们的产品可用于许多种防止RNA降解的反应，例如RT-PCR、cDNA合成、体外转录、酶标记RNA反应或任何其他应用 [2]。
| Components | Size |
| RNase Inhibitor, Murine(40 U/ul) | 3000 U (75 ul) |
| RNase Inhibitor, Murine(40 U/ul) | 15000 U (375 ul) |
| Store the components at -20°C. | |
| 应用&特点 | The product inhibits RNases A, B and C specifically.
Improved resistance to oxidation compared to human/porcine RNase inhibitor. Ideal for reactions when low DTT concentration is necessary (e.g., Real-time PCR). |
| 运输方式 | Ship with blue ice. |
| 储存条件 | Store the components at -20°C. |
| 用途 | 仅供研究使用!不能用于人体。 |