RNase Inhibitor, Murine
目录号 : GK12001鼠源Rnase抑制剂（RNase Inhibitor, Murine）, 是一个50 kD的重组蛋白，从大肠杆菌中分离出来，含有来自小鼠的核糖核酸酶抑制剂基因。
Sample solution is provided at 25 µL, 10mM.
RNase Inhibitor, Murine is a 50 kD recombinant protein isolated from E. coli containing the ribonuclease inhibitor gene originally from mouse. Our product RNase inhibitor has specificity for RNases A, B and C, but couldn’t inhibit RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. It can bind several kinds of RNase non-covalently in a 1:1 ratio with high affinity. Especially, according to previous researches, no inhibition for enzyme activity is observed when RNase Inhibitor is used within AMV or M-MuLV reverse transcriptases, taq DNA polymerase or SP6/ T7/ T3 RNA polymerases, means it can be taken in various experiments to prevent RNA degradation.
Compared to RNase from human, RNase inhibitor from mouse lacks the pair of cysteines which is very sensitive to oxidation and lead to inactivation of the inhibitor. As a consequence, RNase Inhibitor, Murine significantly improves its resistance to oxidation compared to RNase inhibitors from human/porcine. It is stable at low DTT concentrations (less than 1 mM), and this character makes it preferred for reactions where low concentration DTT is necessary to the reaction (eg. Real-time RT-PCR).
Our product can be used in many kinds of reactions to prevent RNA degradation, for example, RT-PCR, cDNA synthesis, In vitro transcription, enzymatic RNA labeling reaction or any other applications.
RNase Inhibitor, Murine是一种50 kD的重组蛋白，最初来自小鼠的核糖核酸酶抑制剂基因，在大肠杆菌中分离得到。我们的产品RNase抑制剂对RNases A、B和C具有特异性，但不能抑制RNase 1、RNase T1、S1核酸酶、RNase H或Aspergillus来源的RNase。它可以与几种不同类型的RNA非共价结合，并且以高亲和力呈现1:1比例。尤其是根据先前的研究，当在AMV或M-MuLV逆转录酶、taq DNA聚合酶或SP6/T7/T3 RNA聚合酶中使用时，没有观察到对于这些酵素活性的抑制作用，这意味着它可以在各种实验中被使用以防止RNA降解。
与人类的核酸酶相比，来自老鼠的核酸酶抑制剂缺少一对半胱氨酸，这对氧化非常敏感并导致抑制剂失活。因此，老鼠RNase Inhibitor在耐氧化性方面显著优于人/猪源RNase抑制剂。它在低DTT浓度（小于1 mM）下稳定，并且这种特性使其成为需要低浓度DTT反应的首选（例如实时RT-PCR）。
我们的产品可以在许多反应中使用，以防止RNA降解，例如RT-PCR、cDNA合成、体外转录、酶标记RNA反应或其他任何应用。
1. Used in IVT(In vitro translation), RT-PCR, cDNA synthesis and other application Add RNase Inhibitor, Murine to achieve a final concentration of 1U/µl in the reaction. Be careful that RNase Inhibitor should be added before other components that may possibly containing RNase (i.e. enzymes, plasmid) Murine RNase Inhibitor is a 50 kDa protein that will be inactivated in denaturing conditions such as high temperature and denaturing agents, it must be used below 50°C.
2. Used in adding poly(A) tail to RNA In the experiment of adding poly(A) tail to RNA, RNase Inhibitor can be added to enhance the stability of the RNA. 0.5 µl of RNase inhibitor to a 20 µl reaction is appropriate when the reaction is set-up. The additional volume can be subtracted from the amount of H2O used in the reaction.
| Components | Size |
| RNase Inhibitor, Murine(40 U/ul) | 3000 U (75 ul) |
| RNase Inhibitor, Murine(40 U/ul) | 15000 U (375 ul) |
| Store the components at -20°C. | |
| 应用&特点 | The product inhibits RNases A, B and C specifically.
Improved resistance to oxidation compared to human/porcine RNase inhibitor. Ideal for reactions when low DTT concentration is necessary (e.g., Real-time PCR). |
| 运输方式 | Ship with blue ice. |
| 储存条件 | Store the components at -20°C. |
| 用途 | 仅供研究使用!不能用于人体。 |
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