Spermidine (N-(4-Aminobutyl)-1,3-diaminopropane)
(Synonyms: 亚精胺) 目录号 : GC31337
Spermidine是一种天然多胺，可通过与DNA/RNA结合并稳定其结构、发挥抗氧化活性、调节酶功能以及参与翻译调控来维持细胞稳态。
Cas No.:124-20-9
Sample solution is provided at 25 µL, 10mM.
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Spermidine, a natural polyamine, binds and stabilizes DNA and RNA, has antioxidative activities, modulates enzyme functions, and is required for the regulation of translation[1-2]. Spermidine, also a natural autophagy inducer, has a variety of health effects, such as antitumor, antiaging, anti-inflammation, cardiovascular protection, and neuromodulation[3]. Spermidine contains three positively charged amino groups, and this structural characteristic is crucial for its functions[3].
In vitro, treatment of human colon carcinoma HCT 116 cells with 100µM Spermidine for 2h induced the dephosphorylation of protein tyrosine kinase 2β (PYK2) and cyclin-dependent kinase inhibitor 1B (p27Kip1)[4]. Exogenous supplementation with 4mM Spermidine on day 1 of aging markedly extended the lifespan of both wild-type BY4741 and DBY746 yeast cells[5].
In vivo, in mice subjected to optic nerve injury (ONI), oral administration of 30mM Spermidine via drinking water prevented the significant loss of retinal ganglion cells (RGCs) and the thinning of the inner retinal layer (IRL) observed in vehicle-treated controls[6]. C57BL/6 WT mice given lifelong access to drinking water containing 0.3mM Spermidine exhibited a significant extension in median lifespan[7]. Continuous administration of 3mM Spermidine in the drinking water of aged mice markedly reduced aortic collagen I expression and restored NO-mediated endothelium-dependent dilation to levels observed in young controls[8].
References:
[1] Hofer SJ, Simon AK, Bergmann M, Eisenberg T, Kroemer G, Madeo F. Mechanisms of spermidine-induced autophagy and geroprotection. Nat Aging. 2022;2(12):1112-1129.
[2] Madeo F, Eisenberg T, Pietrocola F, Kroemer G. Spermidine in health and disease. Science. 2018;359(6374):eaan2788.
[3] Zou D, Zhao Z, Li L, et al. A comprehensive review of spermidine: Safety, health effects, absorption and metabolism, food materials evaluation, physical and chemical processing, and bioprocessing. Compr Rev Food Sci Food Saf. 2022;21(3):2820-2842.
[4] Morselli E, Mariño G, Bennetzen MV, et al. Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome. J Cell Biol. 2011;192(4):615-629.
[5] Eisenberg T, Knauer H, Schauer A, et al. Induction of autophagy by spermidine promotes longevity. Nat Cell Biol. 2009;11(11):1305-1314.
[6] Noro T, Namekata K, Kimura A, et al. Spermidine promotes retinal ganglion cell survival and optic nerve regeneration in adult mice following optic nerve injury. Cell Death Dis. 2015;6(4):e1720.
[7] Eisenberg T, Abdellatif M, Schroeder S, et al. Cardioprotection and lifespan extension by the natural polyamine spermidine. Nat Med. 2016;22(12):1428-1438.
[8] LaRocca TJ, Gioscia-Ryan RA, Hearon CM Jr, Seals DR. The autophagy enhancer spermidine reverses arterial aging. Mech Ageing Dev. 2013;134(7-8):314-320.
Spermidine是一种天然多胺，可通过与DNA/RNA结合并稳定其结构、发挥抗氧化活性、调节酶功能以及参与翻译调控来维持细胞稳态[1-2]。作为一种天然自噬诱导剂，Spermidine具有抗肿瘤、抗衰老、抗炎、心血管保护及神经调节等多重健康效应[3]。Spermidine含三个带正电的氨基，这一结构特征对其功能至关重要[3]。
在体外，用100µM的Spermidine处理人结肠癌HCT 116细胞2小时，可诱导蛋白酪氨酸激酶2β（PYK2）和细胞周期蛋白依赖性激酶抑制剂1B（p27Kip1）去磷酸化[4]。在酵母衰老模型中，衰老第1天添加4mM外源性Spermidine，显著延长野生型BY4741和DBY746菌株的寿命[5]。
在体内，视神经损伤（ONI）小鼠经饮水给予30mM的Spermidine后，显著抑制了视网膜神经节细胞（RGCs）的丢失和内视网膜层（IRL）变薄[6]。C57BL/6野生型小鼠终生饮用含0.3mM的Spermidine的水，中位寿命显著延长[7]。持续给予老年小鼠3mM的Spermidine饮水，可显著降低主动脉Ⅰ型胶原表达，并将NO介导的内皮依赖性舒张恢复至年轻对照水平[8]。
| Cell experiment [1]: | |
Cell lines | Human colon carcinoma HCT 116 cells |
Preparation Method | Human colon carcinoma HCT 116 cells were cultured in McCoy’s 5A medium containing 10% fetal bovine serum, 100mg/liter sodium pyruvate, 10mM Hepes buffer, 100U/ml penicillin G sodium, and 100µg/ml streptomycin sulfate. Cells were seeded in 6- and 12-well plates or in 10 and 15cm dishes and grown for 24h before treatment with 100µM Spermidine for 2h. |
Reaction Conditions | 100μM; 2h |
Applications | Spermidine mediated the dephosphorylation of the protein tyrosine kinase 2β (also known as PYK2) and the cyclin-dependent kinase inhibitor 1B (better known as p27Kip1). |
| Animal experiment [2]: | |
Animal models | C57BL/6J mice |
Preparation Method | Mice were subjected to optic nerve injury (ONI). Spermidine was added to drinking water at 30mM for the treatment groups throughout the whole experimental period. The control group received normal drinking water. Drinking water was replaced every 2-3 days, and Spermidine was freshly added from a 1M aqueous stock (Spermidine/HCl, pH 7.4), which was kept at -20°C for no longer than 1 month. |
Dosage form | 30mM; orally |
Applications | By day 7 (d7) and d14 following optic nerve injury (ONI), the number of retinal ganglion cells (RGCs) was significantly reduced, but Spermidine treatment suppressed such RGC loss. In addition, the thickness of the inner retinal layer (IRL) was significantly decreased in vehicle-treated mice following ONI, whereas this effect was prevented in Spermidine-treated mice. These results suggest that Spermidine protects RGCs from ONI-induced cell death. |
References: | |
| Cas No. | 124-20-9 | SDF | |
| 别名 | 亚精胺 | ||
| Canonical SMILES | NCCCCNCCCN | ||
| 分子式 | C7H19N3 | 分子量 | 145.25 |
| 溶解度 | DMSO : ≥ 33.33 mg/mL (229.47 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 6.8847 mL | 34.4234 mL | 68.8468 mL |
| 5 mM | 1.3769 mL | 6.8847 mL | 13.7694 mL |
| 10 mM | 0.6885 mL | 3.4423 mL | 6.8847 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
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