Pinostrobin
(Synonyms: 球松素) 目录号 : GC40998
Pinostrobin是一种天然类黄酮，具有抗氧化、抗炎、抗病毒和抗癌等多种生物活性。
Cas No.:480-37-5
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
Pinostrobin is a natural flavonoid with various biological activities, including antioxidant, anti-inflammatory, antiviral, and anticancer effects[1, 2]. Pinostrobin can inhibit nuclear factor-kappa B signaling and the production of inflammatory cytokines and chemokines in human macrophages[3].
In vitro, treatment of human cervical cancer HeLa cells with Pinostrobin (10-200µM) reduced cell viability and GSH and NO2- levels in a dose-dependent manner, induced apoptosis, significantly decreased mitochondrial membrane potential, and increased intracellular ROS production[4]. Treatment of 3T3-L1 and MCF-7 cells with Pinostrobin (25µM) significantly reduced adipogenesis in 3T3-L1 cells and inhibited migration and colony formation in MCF-7 cells[5]. Treatment of human acute myeloid leukemia (AML) cells with Pinostrobin (0-80µM) significantly reduced cell survival, with significant cytotoxic effects observed in MV4-11>MOLM-13>HL-60>U-937>THP-1 cells, affecting cell cycle progression and promoting apoptosis[6].
In vivo, treatment of zebrafish larvae with Pinostrobin (0-25µM) significantly reduced α-MSH-induced melanogenesis in a concentration-dependent manner without causing cardiotoxicity[7].
References:
[1] Gu C, Fu L, Yuan X, et al. Promoting effect of pinostrobin on the proliferation, differentiation, and mineralization of murine pre-osteoblastic MC3T3-E1 cells[J]. Molecules, 2017, 22(10): 1735.
[2] Chutiwitoonchai N, Akkarawongsapat R, Chantawarin S, et al. Antiviral effect of pinostrobin, a bioactive constituent of Boesenbergia rotunda, against porcine epidemic diarrhea virus[J]. Antiviral Research, 2025, 234: 106073.
[3] Budluang P, Potikanond S, Wikan N, et al. Pinostrobin Inhibits Nuclear Factor-Kappa B Signaling and Production of Inflammatory Cytokines and Chemokines in Human Macrophages[J]. Nutrients, 2025, 17(22): 3589.
[4] Jaudan A, Sharma S, Malek S N A, et al. Induction of apoptosis by pinostrobin in human cervical cancer cells: Possible mechanism of action[J]. PLoS One, 2018, 13(2): e0191523.
[5] Benchamana A, Tohkayomatee R, Soodvilai S, et al. Pinostrobin exerts inhibitory effects on adipogenesis and adipocyte-induced MCF-7 breast cancer cell proliferation and migration[J]. Trends in Sciences, 2024, 21(9): 8125-8125.
[6] Chen P Y, Lin C Y, Wu C L, et al. Pinostrobin modulates FOXO3 expression, nuclear localization, and exerts antileukemic effects in AML cells and zebrafish xenografts[J]. Chemico-Biological Interactions, 2023, 385: 110729.
[7] Athapaththu A M G K, Sanjaya S S, Lee K T, et al. Pinostrobin suppresses the α-melanocyte-stimulating hormone-induced melanogenic signaling pathway[J]. International Journal of Molecular Sciences, 2023, 24(1): 821.
Pinostrobin是一种天然类黄酮，具有抗氧化、抗炎、抗病毒和抗癌等多种生物活性[1, 2]。Pinostrobin能够抑制核因子-Kappa B信号传导及人类巨噬细胞中炎症细胞因子和化学因子的产生[3]。
在体外，Pinostrobin（10-200µM）处理人宫颈癌细胞HeLa细胞，以剂量依赖性方式降低了细胞活力以及GSH和NO2-水平，诱导了细胞凋亡，显著降低了线粒体膜电位，增加了细胞内ROS的生成[4]。Pinostrobin（25µM）处理3T3-L1细胞和MCF-7细胞，显著降低了3T3-L1细胞的脂肪生成，抑制了MCF-7细胞的迁移和集落形成[5]。Pinostrobin（0-80µM）处理人类急性骨髓性白血病（AML）细胞，显著降低了细胞的存活率，在 MV4-11>MOLM-13>HL-60>U-937>THP-1细胞中观察到明显的细胞毒性效应，影响了细胞周期进程，促进了细胞凋亡[6]。
在体内，Pinostrobin（0-25µM）处理斑马鱼幼体，以浓度依赖性方式显著降低了α-MSH诱导的斑马鱼幼体黑色素沉着，且不引起心脏毒性[7]。
| Cell experiment [1]: | |
Cell lines | HeLa、Ca Ski、SiHa cells |
Preparation Method | Cells were seeded at a density of 5x103 cells/well in 96 well plates and incubated for 24h in CO2 incubator. The cells were then treated with various concentrations of Pinostrobin (10, 30, 50, 100, 200μM, in triplicate). Cells treated with triple solvent (vehicle) and Doxorubicin (DX, 10μM) was included as negative and positive controls, respectively. Cell viability was monitored at 24, 48, 72 and 96h post-treatment using MTT assay essentially. |
Reaction Conditions | 10, 30, 50, 100, 200μM; 24, 48, 72, 96h |
Applications | Of the three different cervical cancer cell lines, Pinostrobin showed highest toxicity for HeLa cells followed by Ca Ski and SiHa. |
| Animal experiment [2]: | |
Animal models | Zebrafish |
Preparation Method | One day post-fertilization (dpf) zebrafish larvae were pretreated with phenylthiourea (PTU) (200µM) for 48h and then incubated with α-MSH (1µg/mL) for an additional 24h. At 4 dpf, the medium was replaced with PTU and α-MSH, and the embryos were treated with Pinostrobin (0-25μM) for 48h (at 6 dpf). After anesthetizing zebrafish larvae with 0.02% tricane methanesulfonate, they were mounted in 2% methyl cellulose on a depression slide, and images were collected using stereomicroscopye. Densitometric analysis was performed using the ImageJ software. The quantification of pigmentation data was calculated as a percentage of untreated zebrafish larvae. |
Dosage form | 0-25μM |
Applications | Pinostrobin markedly decreased α-MSH-induced melanin pigmentation in zebrafish larvae in a concentration-dependent manner. |
References: | |
| Cas No. | 480-37-5 | SDF | |
| 别名 | 球松素 | ||
| Canonical SMILES | COC1=CC(O)=C(C(C[C@@H](C2=CC=CC=C2)O3)=O)C3=C1 | ||
| 分子式 | C16H14O4 | 分子量 | 270.3 |
| 溶解度 | DMF: 20 mg/ml,DMF:PBS (pH 7.2)(1:3): 0.25 mg/ml,DMSO: 12 mg/ml | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.6996 mL | 18.498 mL | 36.9959 mL |
| 5 mM | 739.9 μL | 3.6996 mL | 7.3992 mL |
| 10 mM | 370 μL | 1.8498 mL | 3.6996 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet