α-Factor Mating Pheromone, yeast (Mating Factor α)
(Synonyms: А1-粘合因子乙酸盐,Mating Factor α) 目录号 : GC30587
α-Factor Mating Pheromone, yeast (Mating Factor α)是一种由13个氨基酸组成的肽类激素，由酿酒酵母的MATα型细胞持续合成和分泌，并能特异性地作用于MATa型细胞。
Cas No.:59401-28-4
Sample solution is provided at 25 µL, 10mM.
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α-Factor Mating Pheromone, yeast (Mating Factor α) is a 13-amino acid peptide hormone that is continuously synthesized and secreted by MATα-type cells of Saccharomyces cerevisiae and can specifically act on MATa-type cells [1]. α-Factor Mating Pheromone, yeast (Mating Factor α) binds to the G protein-coupled receptor Ste2p on the surface of MATa cells, triggering cell cycle arrest, morphological changes, and the expression of mating-related genes. This factor can compete with radiolabeled ³⁵S-α-factor for binding (Kd = 0.3μM). The binding of α-Factor Mating Pheromone, yeast (Mating Factor α) to the thermosensitive ste2 mutant is thermally unstable, while the binding to other mutants (ste4, ste5, ste7, ste11 and ste12) is not thermally unstable [2]. α-Factor Mating Pheromone, yeast (Mating Factor α) is often used to induce yeast to enter a mating state and is widely used in the secretion and expression of foreign proteins [3].
References:
[1]. Naider F, Becker JM. The α-factor mating pheromone of Saccharomyces cerevisiae: a model for studying the interaction of peptide hormones and G protein-coupled receptors. Peptides. 2004 Sep 1; 25(9): 1441-1463.
[2]. Jenness DD, Burkholder AC, Hartwell LH. Binding of α-factor pheromone to yeast a cells: chemical and genetic evidence for an α-factor receptor. Cell. 1983 Dec 1; 35(2): 521-529.
[3]. Sieber B, Coronas-Serna JM, Martin SG. A focus on yeast mating: From pheromone signaling to cell-cell fusion. InSeminars in Cell & Developmental Biology 2023 Jan 15; 133: 83-95.
α-Factor Mating Pheromone, yeast (Mating Factor α)是一种由13个氨基酸组成的肽类激素，由酿酒酵母的MATα型细胞持续合成和分泌，并能特异性地作用于MATa型细胞 [1]。α-Factor Mating Pheromone, yeast (Mating Factor α)可与MATa细胞表面的G蛋白偶联受体Ste2p结合，从而引发细胞周期停滞、形态学变化以及交配相关基因的表达。该因子可与放射性标记的³⁵S-α-factor竞争结合（Kd = 0.3μM）。α-Factor Mating Pheromone, yeast (Mating Factor α)与温敏性ste2突变体的结合具有热稳定性，而与其他突变体（ste4、ste5、ste7、ste11和ste12）的结合则不具有热稳定性 [2]。α-Factor Mating Pheromone, yeast (Mating Factor α)常用于诱导酵母进入交配状态，并广泛应用于外源蛋白的分泌和表达 [3]。
| Cas No. | 59401-28-4 | SDF | |
| 别名 | А1-粘合因子乙酸盐,Mating Factor α | ||
| Canonical SMILES | Trp-His-Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro-Met-Tyr | ||
| 分子式 | C82H114N20O17S | 分子量 | 1684 |
| 溶解度 | Soluble in Water | 储存条件 | -20°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 0.5938 mL | 2.9691 mL | 5.9382 mL |
| 5 mM | 0.1188 mL | 0.5938 mL | 1.1876 mL |
| 10 mM | 0.0594 mL | 0.2969 mL | 0.5938 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet