N6-methyladenosine (m6A)
(Synonyms: N6-甲基腺苷; 6-Methyladenosine; N-Methyladenosine) 目录号 : GC14698
N6-methyladenosine (m6A)是腺苷的潜在激动剂，ED50值为17250nM。
Cas No.:1867-73-8
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
N6-methyladenosine (m6A) is a potential agonist of adenosine with an ED50 value of 17250nM[1]. N6-methyladenosine is the methylation occurring at the N6 position of adenine, and is the most common internal modification on eukaryotic mRNA[2]. N6-methyladenosine regulates gene expression, thereby controlling a series of processes such as cell self-renewal, differentiation, invasion and apoptosis[3]. A global decrease in N6-methyladenosine (m6A) results in double-stranded RNA (dsRNA) formation and a profound cell-intrinsic interferon response[4]. The changes in N6-methyladenosine levels are closely related to the clinical and pathological features of gastrointestinal tumors and liver cancer. N6-methyladenosine has become a potential diagnostic and prognostic marker or a promising therapeutic target[5].
References:
[1] Ribeiro J A, Sebastião A M. On the type of receptor involved in the inhibitory action of adenosine at the neuromuscular junction[J]. British journal of pharmacology, 1985, 84(4): 911.
[2] Chen M, Wong C M. The emerging roles of N6-methyladenosine (m6A) deregulation in liver carcinogenesis[J]. Molecular cancer, 2020, 19(1): 44.
[3] He L, Li H, Wu A, et al. Functions of N6-methyladenosine and its role in cancer[J]. Molecular cancer, 2019, 18(1): 176.
[4] Guirguis A A, Ofir-Rosenfeld Y, Knezevic K, et al. Inhibition of METTL3 results in a cell-intrinsic interferon response that enhances antitumor immunity[J]. Cancer Discovery, 2023, 13(10): 2228-2247.
[5] Zhu D H, Su K K, Ou-Yang X X, et al. Mechanisms and clinical landscape of N6-methyladenosine (m6A) RNA modification in gastrointestinal tract cancers[J]. Molecular and Cellular Biochemistry, 2024, 479(7): 1553-1570.
N6-methyladenosine (m6A)是腺苷的潜在激动剂，ED50值为17250nM[1]。作为腺嘌呤N6位点的甲基化修饰，N6-methyladenosine是真核生物mRNA中最常见的内源性化学修饰[2]。N6-methyladenosine通过调控基因表达，参与细胞自我更新、分化、侵袭和凋亡等一系列生物学过程[3]。整体性N6-methyladenosine水平降低会导致双链RNA（dsRNA）形成并引发强烈的细胞内在干扰素反应[4]。N6-methyladenosine水平变化与胃肠道肿瘤和肝癌的临床病理特征密切相关，已成为潜在的诊断预后标志物和治疗靶点[5]。
| Cas No. | 1867-73-8 | SDF | |
| 别名 | N6-甲基腺苷; 6-Methyladenosine; N-Methyladenosine | ||
| 化学名 | (2R,3S,4R,5R)-2-(hydroxymethyl)-5-(6-(methylamino)-9H-purin-9-yl)tetrahydrofuran-3,4-diol | ||
| Canonical SMILES | CNC1=C(N=CN2[C@@]3([H])[C@@](O)([H])[C@@](O)([H])[C@@](O3)([H])CO)C2=NC=N1 | ||
| 分子式 | C11H15N5O4 | 分子量 | 281.27 |
| 溶解度 | ≥ 12.2mg/mL in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.5553 mL | 17.7765 mL | 35.553 mL |
| 5 mM | 711.1 μL | 3.5553 mL | 7.1106 mL |
| 10 mM | 355.5 μL | 1.7777 mL | 3.5553 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet