N-3-hydroxybutyryl-L-Homoserine lactone
(Synonyms: 3-OH-C4-HSL, N-3-hydroxybutanoyl-L-Homoserine lactone, N-3-hydroxybutyryl-L-HSL, N-3-hydroxybutanoyl-L-HSL) 目录号 : GC46181N-3-hydroxybutyryl-L-Homoserine lactone是由Vibrio splendidus细菌产生的N-酰基高丝氨酸内酯，已在用于废水处理的序批式生物膜反应器中被检测到。
Cas No.:1325550-06-8
Sample solution is provided at 25 µL, 10mM.
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N-3-hydroxybutyryl-L-Homoserine lactone is an N-acyl-homoserine lactone produced by the bacterium V. splendidus and has been found in sequencing batch biofilm reactors used in wastewater treatment[1][2]. N-3-hydroxybutyryl-L-Homoserine lactone is a signaling molecule that is involved in bacterial quorum sensing (QS)[3]. N-3-hydroxybutyryl-L-Homoserine lactone senses the density of bacterial population, regulates the cellular process, such as bioluminescence, biofilm formation, and exo-enzyme production[4][5]. N-3-hydroxybutyryl-L-homoserine lactone can be used for screening antimicrobial drugs that interfere with quorum sensing and for studying bacterial infection mechanisms[6][7].
References:
[1] Purohit AA, Johansen JA, Hansen H, et al. Presence of acyl-homoserine lactones in 57 members of the Vibrionaceae family. J Appl Microbiol. 2013;115(3):835-847.
[2] Feng Z, Sun Y, Li T, Meng F, Wu G. Operational pattern affects nitritation, microbial community and quorum sensing in nitrifying wastewater treatment systems. Sci Total Environ. 2019;677:456-465.
[3] Ke X, Miller LC, Bassler BL. Determinants governing ligand specificity of the Vibrio harveyi LuxN quorum-sensing receptor. Mol Microbiol. 2015;95(1):127-142.
[4] Rajamani S, Sayre RT. A sensitive fluorescence reporter for monitoring quorum sensing regulated protease production in Vibrio harveyi. J Microbiol Methods. 2011;84(2):189-193.
[5] Yan G, Fu L, Ming H, Chen C, Zhou D. Exploring an Efficient and Eco-Friendly Signaling Molecule and Its Quorum Quenching Ability for Controlling Microcystis Blooms. Environ Sci Technol. 2023;57(44):16929-16939.
[6] Hanzelka BL, Parsek MR, Val DL, Dunlap PV, Cronan JE Jr, Greenberg EP. Acylhomoserine lactone synthase activity of the Vibrio fischeri AinS protein. J Bacteriol. 1999;181(18):5766-5770.
[7] Sharma N, Pandey S, Yadav M, et al. Biomolecular map of albumin identifies signatures of severity and early mortality in acute liver failure. J Hepatol. 2023;79(3):677-691.
N-3-hydroxybutyryl-L-Homoserine lactone是由Vibrio splendidus细菌产生的N-酰基高丝氨酸内酯，已在用于废水处理的序批式生物膜反应器中被检测到[1][2]。N-3-hydroxybutyryl-L-Homoserine lactone是一种参与细菌群体感应（Quorum Sensing，QS）的信号分子[3]。N-3-hydroxybutyryl-L-Homoserine lactone通过感知细菌群体密度，调控细胞过程，比如生物发光、生物膜形成和胞外酶的产生[4][5]。N-3-hydroxybutyryl-L-Homoserine lactone可用于筛选能够干扰群体感应的抗菌药物，还可用于研究细菌感染机制[6][7]。
| Cas No. | 1325550-06-8 | SDF | |
| 别名 | 3-OH-C4-HSL, N-3-hydroxybutanoyl-L-Homoserine lactone, N-3-hydroxybutyryl-L-HSL, N-3-hydroxybutanoyl-L-HSL | ||
| Canonical SMILES | OC(C)CC(N[C@@H]1C(OCC1)=O)=O | ||
| 分子式 | C8H13NO4 | 分子量 | 187.2 |
| 溶解度 | DMF: soluble; DMSO: soluble | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 5.3419 mL | 26.7094 mL | 53.4188 mL |
| 5 mM | 1.0684 mL | 5.3419 mL | 10.6838 mL |
| 10 mM | 534.2 μL | 2.6709 mL | 5.3419 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet