IR 783
目录号 : GC48378
IR 783是一种近红外（NIR）七甲川菁荧光探针，其最大激发/发射波长分别为633/780nm。
Cas No.:115970-66-6
Sample solution is provided at 25 µL, 10mM.
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IR 783 is a near-infrared (NIR) heptamethine cyanine fluorescent probe with excitation/emission maxima of 633/780nm, respectively[1]. IR 783 can be used in combination with anticancer drugs to enhance their targeting and therapeutic efficacy[2]. IR 783 is usually used in research related to for cell imaging and drug delivery[3][4][5].
In vitro, treatment of MDA-MB-231 and MCF-7 breast cancer cells with IR 783 (0-160µM; 0-72h) inhibited the proliferation and migration, promoted mitochondrial fission, and decreased filopodia formation in a dose- and time-dependent manner[6].
In vivo, IR 783 (20mg/kg/day; intravenous injection; 4 weeks) significantly inhibited tumor growth, increased mitochondrial translocation of Drp1, and activated the mitochondrial apoptotic pathway in an MDA-MB-231 xenograft mouse model[7].
References:
[1] Yuan J, Yi X, Yan F, et al. Near infrared fluorescence imaging of prostate cancer using heptamethine carbocyanine dyes. Mol Med Rep. 2015;11(2):821-828.
[2] Huang QJ, Liao GC, Zhuang XR, et al. Ras inhibitor farnesylthiosalicylic acid conjugated with IR783 dye exhibits improved tumor-targeting and altered anti-breast cancer mechanisms in mice. Acta Pharmacol Sin. 2022;43(7):1843-1856.
[3] Yang X, Shao C, Wang R, et al. Optical imaging of kidney cancer with novel near infrared heptamethine carbocyanine fluorescent dyes. J Urol. 2013;189(2):702-710.
[4] De Los Reyes-Berbel E, Salto-Gonzalez R, Ortega-Muñoz M, et al. PEI-NIR Heptamethine Cyanine Nanotheranostics for Tumor Targeted Gene Delivery. Bioconjug Chem. 2018;29(8):2561-2575.
[5] Yang X, Shi C, Tong R, et al. Near IR heptamethine cyanine dye-mediated cancer imaging. Clin Cancer Res. 2010;16(10):2833-2844.
[6] Li P, Liu Y, Liu W, et al. IR-783 inhibits breast cancer cell proliferation and migration by inducing mitochondrial fission. Int J Oncol. 2019;55(2):415-424.
[7] Tang Q, Liu W, Zhang Q, et al. Dynamin-related protein 1-mediated mitochondrial fission contributes to IR-783-induced apoptosis in human breast cancer cells. J Cell Mol Med. 2018;22(9):4474-4485.
IR 783是一种近红外（NIR）七甲川菁荧光探针，其最大激发/发射波长分别为633/780nm[1]。IR 783可以与抗癌药物联合使用，以增强其靶向性和疗效[2]。IR 783通常用于细胞成像和药物递送相关研究[3][4][5]。
在体外实验中，IR 783（0-160µM；0-72小时）处理MDA-MB-231和MCF-7乳腺癌细胞，以剂量和时间依赖的方式抑制细胞增殖和迁移，促进线粒体分裂，并减少丝状伪足形成[6]。
在体内实验中，IR 783（20mg/kg/天；静脉注射；4周）显著抑制了MDA-MB-231异种移植小鼠模型中的肿瘤生长，增加了Drp1的线粒体转位，并激活了线粒体凋亡途径[7]。
| Cell experiment [1]: | |
Cell lines | MDA-MB-231 and MCF-7 breast cancer cells |
Preparation Method | The human breast cancer cell lines MDA-MB-231 and MCF-7 were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum (FBS). Cells were cultured at 37˚C with 5% CO2 and digested every 2 days by 0.25% trypsin. Cells (2×104 cells/well) were seeded onto 96-well plates and allowed to attach. After treatment with various concentrations of IR 783 (0, 20, 40, 60, 80, 100, 120, 140 and 160µM) for 24h or with 80µM IR 783 for different time intervals (0, 3, 6, 12, 24, 36, 48 and 72h) in a 5% CO2 incubator at 37˚C. The proliferation of cells was determined by MTT assay. Cell cycle analysis was performed by flow cytometry. The migration ability of MDA-MB-231 cells was assessed using a wound healing assay. The morphology of mitochondria was studied using a Tecnai 10 transmission electron microscope. |
Reaction Conditions | 0-160µM; 0-72h |
Applications | Treatment of MDA-MB-231 and MCF-7 breast cancer cells with IR 783 inhibited the proliferation and migration, promoted mitochondrial fission, and decreased filopodia formation in a dose- and time-dependent manner. |
| Animal experiment [2]: | |
Animal models | Female nude mice |
Preparation Method | Female nude mice (5 weeks old) were used in this experiment. MDA-MB-231 cells were mixed with Matrigel (1:1) and injected subcutaneously into the right hind legs of nude mice. Mice were randomized into 2 groups (n=5). IR 783 (20mg/kg) or an equal volume of vehicle was administered daily by intravenous injection starting 7 days after tumour inoculation. Tumour growth and mice body weights were measured every week, and tumour volume was calculated as (length×width2)/2. After 4 weeks of treatment, mice were killed by cervical dislocation, and tumour tissues were harvested and fixed in 4% paraformaldehyde or frozen at-80°C. Histological, immunohistochemical and immunofluorescence analyses were performed. TdT-mediated dUTP nick-end labelling (TUNEL) analysis was used to detect the dead cells in the tissue samples. Tumour tissues from each group were lysed and subjected to western blot analysis. |
Dosage form | 20mg/kg/day; intravenous injection; 4 weeks |
Applications | IR 783 significantly inhibited tumor growth, increased mitochondrial translocation of Drp1, and activated the mitochondrial apoptotic pathway in an MDA-MB-231 xenograft mouse model. |
References: | |
| Cas No. | 115970-66-6 | SDF | |
| Canonical SMILES | ClC1=C(CCC/C1=C\C=C2C(C)(C)C3=CC=CC=C3N\2CCCCS([O-])(=O)=O)/C=C/C4=[N+](CCCCS([O-])(=O)=O)C5=CC=CC=C5C4(C)C.[Na+] | ||
| 分子式 | C38H46ClN2O6S2•Na | 分子量 | 749.4 |
| 溶解度 | DMF: 1 mg/ml,DMSO: 1 mg/ml,Ethanol: 1 mg/ml,PBS (pH 7.2): 1 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.3344 mL | 6.672 mL | 13.344 mL |
| 5 mM | 266.9 μL | 1.3344 mL | 2.6688 mL |
| 10 mM | 133.4 μL | 667.2 μL | 1.3344 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
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