Guanylic acid (5'-GMP)
(Synonyms: 鸟苷酸; 5'-GMP; 5'-guanosine monophosphate) 目录号 : GC31562
Guanylic acid (5'-GMP)是一种重要的核苷酸类化合物，是RNA的组成成分。
Cas No.:85-32-5
Sample solution is provided at 25 µL, 10mM.
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Guanylic acid (5'-GMP) is an important nucleotide compound and a component of RNA[1-2]. Guanylic acid is involved in various metabolic pathways and exhibits certain biological activities, such as potential roles in regulating nutrient metabolism and enhancing immunity[3-4].
In vitro, treating L5178Y cells with Guanylic acid (40-200μM) and Bredinin (50-100μM) for 16–40 hours reversed the inhibitory effects of Bredinin on nucleic acid synthesis and chromosomal aberrations in L5178Y cells. Guanylic acid also caused an increase in cell volume and inhibited cell proliferation in L5178Y cells[5].
In vivo, treating adult male Wistar rats with Guanylic acid (10–150mg/kg; intraperitoneal injection) 1 hour prior to testing significantly increased anxiolytic-like behavior. Guanylic acid did not alter cerebrospinal fluid purine concentration or locomotor activity in the rat[6].
References:
[1] Tansutaphanit S, Haga Y, Kabeya N, et al. Impact of purine nucleotide on fatty acid metabolism and expression of lipid metabolism-related gene in the liver cell of rainbow trout Oncorhynchus mykiss. Comp Biochem Physiol B Biochem Mol Biol. 2023 Jun-Jul;266:110845.
[2] FAIRLEY JL Jr, LORING HS. Growth-promoting activities of guanine, guanosine, guanylic acid, and xanthine for a purine-deficient strain of Neurospora. J Biol Chem. 1949 Jan;177(1):451-9.
[3] Ni X, Han Y, Yu J, et al. Structural basis of the C-terminal domain of SARS-CoV-2 N protein in complex with GMP reveals critical residues for RNA interaction. Bioorg Med Chem Lett. 2024 Dec 1;114:130014.
[4] Molz S, Dal-Cim T, Tasca CI. Guanosine-5'-monophosphate induces cell death in rat hippocampal slices via ionotropic glutamate receptors activation and glutamate uptake inhibition. Neurochem Int. 2009 Dec;55(7):703-9.
[5] Sakaguchi K, Tsujino M, Hayashi M, et al. Mode of action of bredinin with guanylic acid on L5178Y mouse leukemia cells. J Antibiot (Tokyo). 1976 Dec;29(12):1320-7.
[6] Almeida RF, Cereser VH Jr, Faraco RB, et al. Systemic administration of GMP induces anxiolytic-like behavior in rats. Pharmacol Biochem Behav. 2010 Sep;96(3):306-11.
Guanylic acid (5'-GMP)是一种重要的核苷酸类化合物，是RNA的组成成分[1-2]。Guanylic acid与多种代谢途径有关，并具有一定的生物活性，如研究表明其可能具有调节机体营养代谢、提高免疫力等潜力[3-4]。
在体外，Guanylic acid（40-200μM）与Bredinin（50-100μM）共同处理L5178Y细胞16–40小时，可逆转Bredinin对L5178Y细胞核酸合成的抑制及染色体畸变，Guanylic acid还引起L5178Y细胞体积增大并抑制细胞增殖[5]。
在体内，Guanylic acid（10–150mg/kg；腹腔注射）处理成年雄性Wistar大鼠1小时，Guanylic acid显著增加小鼠的的抗焦虑样行为，且Guanylic acid未改变小鼠脑脊液嘌呤浓度或运动活性[6]。
| Cell experiment [1]: | |
Cell lines | L5178Y mouse leukemia cells |
Preparation Method | L5178Y cells were maintained in Fischer's medium supplemented with 10% dialyzed bovine serum, penicillin, and streptomycin at 37°C. Cells were treated with Guanylic acid at concentrations of 40-200μM, alone or in combination with Bredinin (50μM to 100μM), for 16–40 hours. |
Reaction Conditions | 40-200μM; 16–40h |
Applications | Guanylic acid reversed Bredinin-induced inhibition of nucleic acid synthesis and chromosomal aberrations in L5178Y cells. In combination with high-dose Bredinin (50μM), Guanylic acid induced cytostatic effects, leading to a 70% increase in cell volume and elevated protein content. |
| Animal experiment [2]: | |
Animal models | Wistar rats |
Preparation Method | Rats were intraperitoneally administered Guanylic acid at doses of 10, 25, 50, 100, or 150mg/kg 1 hour before behavioral testing. Diazepam (2mg/kg) served as a positive control. Anxiety-like behaviors were evaluated using the light/dark task, elevated plus-maze, and open field tasks. Cerebrospinal fluid (CSF) was collected 1 hour post-injection for purine analysis via HPLC. |
Dosage form | 10–150mg/kg; i.p.; Single injection. |
Applications | Guanylic acid (25 and 50mg/kg) induced anxiolytic-like effects comparable to Diazepam, Guanylic acid significantly increasing time spent in the light compartment of the light/dark apparatus and transitions between light/dark chambers. In the elevated plus-maze, Guanylic acid (25 and 50mg/kg) increased time spent in open arms, without altering locomotor activity or entries into closed/total arms. |
References: | |
| Cas No. | 85-32-5 | SDF | |
| 别名 | 鸟苷酸; 5'-GMP; 5'-guanosine monophosphate | ||
| Canonical SMILES | O=P(O)(OC[C@@H]1[C@H]([C@H]([C@H](N2C=NC3=C2N=C(N)NC3=O)O1)O)O)O | ||
| 分子式 | C10H14N5O8P | 分子量 | 363.22 |
| 溶解度 | 50 mg/mlWater | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.7532 mL | 13.7658 mL | 27.5315 mL |
| 5 mM | 550.6 μL | 2.7532 mL | 5.5063 mL |
| 10 mM | 275.3 μL | 1.3766 mL | 2.7532 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
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