N1-Acetylspermidine (hydrochloride)
(Synonyms: N-乙酰精脒盐酸盐) 目录号 : GC45518
N1-Acetylspermidine (hydrochloride)是亚精胺的一种乙酰化衍生物，可作为多胺氧化酶（PAO）的底物。
Cas No.:34450-16-3
Sample solution is provided at 25 µL, 10mM.
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N1-Acetylspermidine (hydrochloride) is an acetyl derivative of spermidine that acts as a substrate for polyamine oxidase (PAO)[1]. The level of N1-Acetylspermidine in tumor tissues has significantly increased, which acts as a novel biomarker of T-lymphoblastic lymphoma/acute leukemia (T-LBL/ALL) [2]. N1-Acetylspermidine has been used to examine the interaction between DNA and polyamines during the cleavage of phosphodiester bonds at apurinic/apyrimidinic sites in DNA[3].
In vitro, N1-Acetylspermidine treatment (100μM; 8h) increased the cell migration ability of HL-60 cells[4]. Treatment with 500µM N1-Acetylspermidine for 24 hours significantly induced apoptosis in SW620 cells in the presence of apple procyanidin[5].
In vivo, N1-Acetylspermidine was injected intraperitoneally once at a dose of 30mg/kg every three days for a total of 9 days, which significantly promoted the growth of liver cancer in mice with xenografted Hepa1-6 tumors[6].
References:
[1] Takao K, Sugita Y, Shirahata A. Assay of N1-acetylpolyamine oxidase activity with N1, N11-didansylnorspermine as the substrate by ion-pair reversed phase high performance liquid chromatography[J]. Biological and Pharmaceutical Bulletin, 2010, 33(7): 1089-1094.
[2] Liu Q, Zheng W, Yang Y, et al. Metabolomic Profiling Identifies Serum N1-Acetylspermidine As a Tumor-Specific Prognostic Biomarker for T-Lymphoblastic Lymphoma/Acute Leukemia[J]. Blood, 2024, 144: 5925.
[3] Haukanes B I, Szajko K, Helland D E. Action of spermidine, N1‐acetylspermidine, and N8acetylspermidine at apurinic sites in DNA[J]. FEBS letters, 1990, 269(2): 389-393.
[4] Kato M, Maeda K, Nakahara R, et al. Acidic extracellular pH drives accumulation of N1-acetylspermidine and recruitment of protumor neutrophils[J]. PNAS nexus, 2023, 2(10): pgad306.
[5] Gossé F, Roussi S, Guyot S, et al. Potentiation of apple procyanidin-triggered apoptosis by the polyamine oxidase inactivator MDL 72527 in human colon cancer-derived metastatic cells[J]. International journal of oncology, 2006, 29(2): 423-428.
[6] Liu Z Y, Wu C Y, Wu R Q, et al. Efflux of N1-acetylspermidine from hepatoma fosters macrophage-mediated immune suppression to dampen immunotherapeutic efficacy[J]. Immunity, 2025, 58(6): 1572-1585. e10.
N1-Acetylspermidine (hydrochloride)是亚精胺的一种乙酰化衍生物，可作为多胺氧化酶（PAO）的底物[1]。N1-Acetylspermidine在肿瘤组织中的水平显著升高，可作为T淋巴母细胞淋巴瘤/急性白血病（T-LBL/ALL）的新型生物标志物[2]。N1-Acetylspermidine已被用于研究DNA中脱嘌呤/脱嘧啶位点磷酸二酯键断裂过程中DNA与多胺之间的相互作用[3]。
在体外，使用100µM的N1-Acetylspermidine处理8小时，增强了HL-60细胞的迁移能力[4]。在苹果原花青素存在下，使用500µM的N1-Acetylspermidine处理24小时，显著诱导了SW620细胞凋亡[5]。
在体内，每隔三天腹腔注射一次N1-Acetylspermidine，剂量为30mg/kg，共计9天，显著促进了携带Hepa1-6肿瘤异种移植小鼠的肝癌生长[6]。
| Cell experiment [1]: | |
Cell lines | HL-60 cells |
Preparation Method | 1×106 HL-60 cells were inoculated into the upper chamber of the 8µm pore size transwell well. 200µl of RPMI 1640 medium containing 1% FBS was added to the upper chamber. 500µl of RPMI 1640 medium containing 10% FBS was added to the lower chamber. After culturing for 8 hours under the condition of 100µM N1-Acetylspermidine, the number of migrated cells in the lower chamber was counted. |
Reaction Conditions | 100µM; 8h |
Applications | N1-Acetylspermidine treatment increased the cell migration ability of HL-60 cells. |
| Animal experiment [2]: | |
Animal models | Wild-type C57BL/6 mice |
Preparation Method | Wild-type C57BL/6 mice (6-8 weeks old) were raised in a specific pathogen-free (SPF) environment. 1×106 Hepa1-6 cells were inoculated into the mice. 15 days later, PBS or N1-Acetylspermidine was injected intraperitoneally at a dose of 30mg/kg every 3 days for a total of 9 days. Then, the tumor volume was analyzed. |
Dosage form | 30mg/kg; every 3 days for a total of 9 days; i.p. |
Applications | N1-Acetylspermidine treatment promoted the growth of liver cancer in mice with xenografted Hepa1-6 tumors. |
References: | |
| Cas No. | 34450-16-3 | SDF | |
| 别名 | N-乙酰精脒盐酸盐 | ||
| Canonical SMILES | CC(NCCCNCCCCN)=O.Cl.Cl | ||
| 分子式 | C9H21N3O.2HCl | 分子量 | 260.2 |
| 溶解度 | DMSO: 0.1 mg/ml,PBS (pH 7.2): 10 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.8432 mL | 19.216 mL | 38.432 mL |
| 5 mM | 768.6 μL | 3.8432 mL | 7.6864 mL |
| 10 mM | 384.3 μL | 1.9216 mL | 3.8432 mL |
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| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
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| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >98.00%
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