Phytic acid dodecasodium salt hydrate
(Synonyms: 植酸十二钠盐; Inositol hexaphosphate dodecasodium hydrate) 目录号 : GC36903
Phytic acid是一种来源于豆科植物种子的口服活性化合物。
Cas No.:123408-98-0
Sample solution is provided at 25 µL, 10mM.
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Phytic acid is an orally active compound derived from legume seeds [1]. Phytic acid and its degradation products can scavenge free radicals and reduce oxidative stress [2]. Phytic acid chelation of metal ions (such as iron) can reduce metal-catalyzed oxidative reactions [3]. Phytic acid exhibits anticancer and antioxidant properties [4].
In MC65 cells, Cell survival rate was significantly improved after Phytic acid (100μM; 4d) treatment [5]. In HepG2 cells, Phytic acid (0.1-4mM; 24-48h) treatment inhibited cell growth [6].
In dextran sulfate sodium (DSS)-induced UC mice model, Phytic acid (0.25g/kg; ig; 14d) treatment improved weight loss, shortened colon length, improved clinical scores, and reduced the release of proinflammatory factors (Il-1β, Il-6, and TNF-α) [7]. In a high-fat diet mice model, Phytic acid (0.25-2mg/mL; po; 13 weeks) significantly inhibited obesity and alleviated hepatic steatosis [8].
References:
[1]. Oatway L, Vasanthan T, Helm J H. Phytic acid[J]. Food Reviews International, 2001, 17(4): 419-431.
[2]. Graf E, Empson K L, Eaton J W. Phytic acid. A natural antioxidant[J]. Journal of Biological Chemistry, 1987, 262(24): 11647-11650.
[3]. Lee B J, Hendricks D G. Metal‐catalyzed oxidation of ascorbate, deoxyribose and linoleic acid as affected by phytic acid in a model system[J]. Journal of Food Science, 1997, 62(5): 935-984.
[4]. Bloot A P M, Kalschne D L, Amaral J A S, et al. A review of phytic acid sources, obtention, and applications[J]. Food Reviews International, 2023, 39(1): 73-92.
[5]. Anekonda T S, Wadsworth T L, Sabin R, et al. Phytic acid as a potential treatment for Alzheimer's pathology: evidence from animal and in vitro models[J]. Journal of Alzheimer’s disease, 2011, 23(1): 21-35.
[6]. Al-Fatlawi A A, Al-Fatlawi A A, Irshad M, et al. Rice bran phytic acid induced apoptosis through regulation of Bcl-2/Bax and p53 genes in HepG2 human hepatocellular carcinoma cells[J]. Asian Pacific Journal of Cancer Prevention, 2014, 15(8): 3731-3736.
[7]. Hou X, Sang Y, Dong L. The improved effect and its mechanism of phytic acid on DSS-induced UC mice[J]. Life sciences, 2022, 311: 121139.
[8]. Ran X, Hu G, He F, et al. Phytic acid improves hepatic steatosis, inflammation, and oxidative stress in high-fat diet (HFD)-fed mice by modulating the gut–liver axis[J]. Journal of Agricultural and Food Chemistry, 2022, 70(36): 11401-11411.
Phytic acid是一种来源于豆科植物种子的口服活性化合物 [1]。Phytic acid及其降解产物可以清除自由基，降低氧化应激 [2]。Phytic acid与金属离子（如铁）的螯合可以降低金属催化的氧化反应 [3]。Phytic acid具有抗癌和抗氧化作用 [4]。
在MC65细胞中，Phytic acid（100μM；4d）处理后细胞存活率显著提高 [5]。在HepG2细胞中，Phytic acid（0.1-4mM；24-48h）处理可抑制细胞生长 [6]。
在葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎（UC）小鼠模型中，Phytic acid（0.25g/kg；ig；14d）治疗可改善体重减轻、缩短结肠长度、改善临床评分，并减少促炎因子（IL-1β、IL-6和TNF-α）的释放 [7]。在高脂饮食小鼠模型中，Phytic acid（0.25-2mg/mL；po；13周）显著抑制肥胖并减轻肝脏脂肪变性 [8]。
| Cell experiment [1]: | |
Cell lines | MC65 cells |
Preparation Method | Cell viability was determined using an MTS assay and a calcium ion assay. At 90% confluence, cells were harvested from TC flasks using 0.05% trypsin/EDTA, washed twice with PBS, and seeded at 15 × 103 cells/well in 96-well plates in OPTIMem medium containing (Tet+) or (Tet-) tetracycline (1μg/mL) and excipients, or phytic acid (100μM). Cells grown under Tet- conditions typically died after approximately 3–4 days of culture, accompanied by expression of endogenous A oligomers, whereas cells grown under Tet+ conditions remained viable during this period. Wells containing growth medium without cells served as background controls, and wells containing vehicle served as positive controls. Each treatment was replicated in 3–6 wells, and the treatment duration for each experiment was 72 hours. Absorbance was measured at a wavelength of 490nm using a Spectra Max PLUS microplate reader. The cell viability under different treatments was determined relative to the cell viability under Tet + treatment. The experiments were repeated at least three times. |
Reaction Conditions | 100μM; 4d |
Applications | Cell survival rate was significantly improved after Phytic acid treatment. |
| Animal experiment [2]: | |
Animal models | Dextran sulfate sodium (DSS)-induced UC mice model |
Preparation Method | The C57BL/6 mice were randomly divided into four groups of five mice each. The specific groups were as follows: no treatment group (NT group), Phytic acid feeding group (Phytic acid group), DSS modeling group (DSS group), and Phytic acid treatment group (Phytic acid + DSS group). The mice in the Phytic acid and Phytic acid + DSS groups were gavaged with Phytic acid (0.25g/kg/day) for 21days. After receiving Phytic acid for 14days, mice in the DSS and Phytic acid + DSS groups were given 2.5% DSS aqueous solution for 7days to develop the mouse model of UC. The body weights of all mice were recorded from day 1 of the DSS feeding. Following the completion of the DSS induction period, all mice were euthanized, and colon samples were collected for subsequent studies. |
Dosage form | 0.25g/kg; ig; 14d |
Applications | Phytic acid treatment improved weight loss, shortened colon length, improved clinical scores, and reduced the release of proinflammatory factors (Il-1β, Il-6, and TNF-α). |
References: | |
| Cas No. | 123408-98-0 | SDF | |
| 别名 | 植酸十二钠盐; Inositol hexaphosphate dodecasodium hydrate | ||
| Canonical SMILES | O=P(O[Na])(O[Na])O[C@H]1[C@H]([C@H]([C@H](OP(O[Na])(O[Na])=O)[C@@H](OP(O[Na])(O[Na])=O)[C@@H]1OP(O[Na])(O[Na])=O)OP(O[Na])(O[Na])=O)OP(O[Na])(O[Na])=O.[x].O.[12Na] | ||
| 分子式 | C6H8Na12O25P6 | 分子量 | 942.833 |
| 溶解度 | Water: ≥ 250 mg/mL | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 1.0606 mL | 5.3032 mL | 10.6063 mL |
| 5 mM | 212.1 μL | 1.0606 mL | 2.1213 mL |
| 10 mM | 106.1 μL | 530.3 μL | 1.0606 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet