Ercalcidiol
(Synonyms: 25-羟基维生素; 25-hydroxy Vitamin D2) 目录号 : GC13804
Ercalcidiol是一种可反映体内维生素D营养水平的维生素D2代谢物。
Cas No.:21343-40-8
Sample solution is provided at 25 µL, 10mM.
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Ercalcidiol is a metabolite of vitamin D2 that reflects the nutritional status of vitamin D in the body[1]. Compared to vitamin D3 metabolites, Ercalcidiol has a shorter half-life and exhibits lower binding affinity to vitamin D-binding protein. Due to its relatively weaker potency, higher doses are required when used for therapeutic purposes[2]. Ercalcidiol is commonly employed for monitoring the efficacy of vitamin D2 treatment and managing disorders of calcium and phosphate homeostasis[3,4].
In vitro, Ercalcidiol (292.5nM) was incubated as a substrate in a renal homogenate system from rachitic chickens for 2h, where it was successfully metabolized by 1α-hydroxylase to produce 1α,25-(OH)2D2[5].
References:
[1] TUGCU G, CHAREHSAZ M, AYDIN A. Toxicological evaluation of ergocalciferol, cholecalciferol, and their metabolites by a category approach[J]. Drug and Chemical Toxicology, 2021, 44(6): 661-667.
[2] NEWMAN M S, BRANDON T R, GROVES M N, et al. A liquid chromatography/tandem mass spectrometry method for determination of 25-hydroxy vitamin D2 and 25-hydroxy vitamin D3 in dried blood spots: a potential adjunct to diabetes and cardiometabolic risk screening[J]. Journal of Diabetes Science and Technology, 2009, 3(1): 156-162.
[3] ADAMS J S, SHIEH A, BISHOP C W. Calcifediol as a therapeutic[M]//Feldman and Pike's Vitamin D. 5th ed. Cambridge: Academic Press, 2024: 457-474.
[4] JIN Z, BERTHOLF R L, YI X. Advances and challenges in the measurement of 1, 25-dihydroxyvitamin D: A comprehensive review[J]. Critical Reviews in Clinical Laboratory Sciences, 2023, 60(7): 535-548.
[5] HUGHES M R, BAYLINK D J, JONES P G, et al. Radioligand receptor assay for 25-hydroxyvitamin D2/D3 and 1 alpha, 25-dihydroxyvitamin D2/D3[J]. The Journal of Clinical Investigation, 1976, 58(1): 61-70.
Ercalcidiol是一种可反映体内维生素D营养水平的维生素D2代谢物[1]。Ercalcidiol较维生素D3代谢物半衰期更短，且与维生素D结合蛋白的结合力低于维生素D3，Ercalcidiol较弱的效力在用于治疗时需要更高的剂量[2]。Ercalcidiol通常用于维生素D2治疗效果的监测以及钙磷稳态障碍的治疗[3,4]。
在体外，Ercalcidiol（292.5nM）作为底物在佝偻病鸡肾脏匀浆系统中孵育2h，成功被1α-羟化酶代谢，生成了1α,25-(OH)2D2[5]。
| Cas No. | 21343-40-8 | SDF | |
| 别名 | 25-羟基维生素; 25-hydroxy Vitamin D2 | ||
| 化学名 | (1S,3Z)-3-[(2E)-2-[(1R,3aS,7aR)-1-[(E,2R,5S)-6-hydroxy-5,6-dimethylhept-3-en-2-yl]-7a-methyl-2,3,3a,5,6,7-hexahydro-1H-inden-4-ylidene]ethylidene]-4-methylidenecyclohexan-1-ol | ||
| Canonical SMILES | CC(C=CC(C)C(C)(C)O)C1CCC2C1(CCCC2=CC=C3CC(CCC3=C)O)C | ||
| 分子式 | C28H44O2 | 分子量 | 412.66 |
| 溶解度 | DMF: 20 mg/ml,DMSO: 20 mg/ml,Ethanol: 20 mg/ml,Ethanol:PBS (pH 7.2)(1:2): 0.3 mg/ml | 储存条件 | Store at -20°C,unstable in solution, ready to use. |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.4233 mL | 12.1165 mL | 24.233 mL |
| 5 mM | 484.7 μL | 2.4233 mL | 4.8466 mL |
| 10 mM | 242.3 μL | 1.2117 mL | 2.4233 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet